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Expression analysis of RUS1 and construction of RUS1 plant expressing vector |
| Qiaoyun WENG1,Jihong XING1,Jingao DONG1,Zhiyong LI2,Zhiping DONG2, |
| 1.Molecular Plant Pathology
Lab., College of Life Science, Agricultural University of Hebei, Baoding
071001, China; 2.National Millet Improvement
Center, Institute of Millet Crops, Hebei Academy of Agriculture and
Forestry Sciences, Shijiazhuang 050000, China; |
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Abstract RUS1 was one of the disease resistance gene analogs obtained from Setaria italica Beauv. Semi-quantitative RT-PCR analysis result showed that RUS1 gene could be induced by Uromyces setariae-italicae and had relation to the resistance response of Setaria italica Beauv. against Uromyces setariae-italicae infection. Promoter sequence of RUS1 was obtained by the method of Genome Walking, and its length was 675 bp. RUS1 promoter and pCAMBIA1300 vector were fused to construct RUS1∷GUS vector. GUS histochemical staining result showed that promoter could activate gene expression. RUS1 gene (including the promoter sequence) was obtained through PCR amplification and then fused with pCAMBIA1300 vector to construct pCAMBIA1300∶RUS1 plant expressing vector. The research laid a foundation for gene functional identification of RUS1.
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| Keywords
Setaria italica
RUS1
semi-quantitative RT-PCR
GUS histochemical staining
plant expressing vector
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Issue Date: 05 March 2010
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