Effects of drought on electrical impedance spectroscopy parameters in stems of <italic>Pinus bungeana</italic> Zucc. seedlings

doi:10.1007/s11703-010-1045-3

Front Agric Chin

2010, Vol. 4

Issue (4) : 468-474 https://doi.org/10.1007/s11703-010-1045-3

RESEARCH ARTICLE

Effects of drought on electrical impedance spectroscopy parameters in stems of Pinus bungeana Zucc. seedlings

Aifang WANG¹, Gang ZHANG²(

)

1. College of Forestry, Agricultural University of Hebei, Baoding 071001, China; 2. College of Horticulture, Agricultural University of Hebei, Baoding 071001, China

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Abstract

The effects of drought during preplanting (three treatments: soil relative water content (RWC) 75%–80%, 55%–60%, 35%–40%; B1, 2, and 3, respectively) and postplanting (four treatments: RWC 75%–80%, 55%–60%, 35%–40%, 15%–20%; A1, 2, 3, and 4, respectively) on electrical impedance spectroscopy (EIS) parameters in the stems of Pinus bungeana Zucc. seedlings were investigated by using 4-year-old container seedlings. Stem impedance spectra were modeled by a distributed circuit element model (2-DCE), which showed the extracellular and intracellular resistance (r_e and r_i), relaxation time (τ₁ and τ₂), and distribution coefficient (ψ₁ and ψ₂) of relaxation time. After preplanting B3 drought treatment, r_e and r_i increased significantly with the increase of soluble sugar of the stem, measured by enthronlsulphuric acid method. After four weeks postplanting A4 drought treatment, relative conductivity, and soluble sugar of stem increased significantly, and r_e of stem decreased significantly and continually, indicating that the cell membrane of stem cells was disrupted by severe drought. After five weeks drought treatment, τ₁ of stem under A4 treatment decreased significantly, and ψ₂ of stem under A2, A3, and A4 treatments was higher than that of A1 treatment. Briefly, drought made r_e, r_i, τ₁, and ψ₂ of stem change regularly, but r_e was found to be the most informative and useful parameter measured if used as a single index to assess the drought resistance of P. bungeana Zucc. seedlings.

Keywords drought Pinus bungeana Zucc. stem extracellular resistance intracellular resistance relaxation time

Corresponding Author(s): ZHANG Gang,Email:zhanggang1210@126.com

Issue Date: 05 December 2010

Cite this article:

Aifang WANG,Gang ZHANG. Effects of drought on electrical impedance spectroscopy parameters in stems of Pinus bungeana Zucc. seedlings[J]. Front Agric Chin, 2010, 4(4): 468-474.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1045-3
https://academic.hep.com.cn/fag/EN/Y2010/V4/I4/468

Fig.1 Effects of preplanting drought treatments on electrical impedance spectroscopy (EIS) parameters of stems in Zucc. seedlings
Note: B1: 75%-80% soil RWC, B2: 55%-60%, and B3: 35%-40%.

Fig.2 Effects of preplanting drought treatments on water content of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.

Fig.3 Effects of preplanting drought treatments on soluble sugar concentration of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.

Fig.4 Effects of preplanting drought treatments on relative conductivity of stems in Zucc. seedlings
Note: B1: 75%-80%, B2: 55%-60%, and B3: 35%-40%.

Fig.5 Effects of postplanting drought treatments on extracellular resistance of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80% soil RWC, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.6 Effects of postplanting drought treatments on intracellular resistance of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.7 Effects of postplanting drought treatments on relaxation time ( and ) of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.8 Effects of postplanting drought treatments on the distribution coefficient ( and ) of relaxation time ( and ) of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.9 Effects of postplanting drought treatments on the water content of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.10 Effects of postplanting drought treatments on soluble sugar concentration of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

Fig.11 Effects of postplanting drought treatments on the relative conductivity of stems in Zucc. seedlings with the phase of drought treatments
Note: A1-A4 are postplanting drought treatments, A1: 75%-80%, A2: 55%-60%, A3: 35%-40%, and A4: 15%-20%.

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