Effects of folic acid supplementation on growth performance and hepatic folate metabolism-related gene expressions in weaned piglets

doi:10.1007/s11703-010-1047-1

Front Agric Chin

2010, Vol. 4

Issue (4) : 494-500 https://doi.org/10.1007/s11703-010-1047-1

RESEARCH ARTICLE

Effects of folic acid supplementation on growth performance and hepatic folate metabolism-related gene expressions in weaned piglets

Bing YU^1,2, Guangbo YANG^1,2, Jingbo LIU^1,2, Daiwen CHEN^1,2(

)

1. Key Laboratory for Animal Nutrition of Disease Resistance, Ya’an 625014, China; 2. Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China

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Abstract

The present study was conducted to evaluate the effects of different folic acid supplemental levels on growth performance, serum biochemical indicators, and hepatic folate metabolism-related gene expressions in weaned piglets. There were 160 piglets with initially average bodyweight of 7.33 kg randomly assigned to diets containing five levels of folic acid: basal diets (C), 0.5 mg·kg^-1 folic acid (FS 0.5), 2.5 mg·kg^-1 folic acid (FS 2.5), 5.0 mg·kg^-1 folic acid (FS 5.0), or 10.0 mg·kg^-1 folic acid (FS 10.0). Blood samples were collected from a subset (n = 20; 4 pigs per treatment) of the piglets on day 0, 14, and 28. Liver samples were collected from the blood-taken piglets on day 28 of the experiment. Pigs fed basal diet supplemented with 2.5 mg·kg^-1 folic acid grew faster (P<0.05) and consumed more feed (P<0.01) than groups of C, FS 5.0, and FS 10.0 during the last two weeks. Dietary treatment had no effect on F/G throughout the experiment. Pigs in the FS 2.5 group showed greater concentrations of Growth Hormone (GH) (P<0.05) and Insulin-like Growth Factors (IGF-1) (P<0.01) in serum than C and FS 10.0 on day 28. RT-PCR analysis revealed that FS 0.5, FS 2.5, and FS 5.0 had a greater abundance of the mRNA encoding 5,10-methylenetetrahydrofolate reductase than C and FS 10.0 (P<0.01). The mRNA expressions of folate binding protein in FS 0.5 and FS 2.5 were upregulated compared with pigs fed with basal diet (P<0.05). These results demonstrated that folate supplemental level of 2.5 mg·kg^-1 significantly enhanced the growth performance of piglets. Folic acid had an impact on folate metabolism and the homocysteine concentrations. No folate supplementation or folate supplemental level of 10 mg·kg^-1 could not increase the growth performance to the greatest degree.

Keywords folic acid growth performance folate binding protein (FOLBP) 5,10-methylenetetrahydrofolate reductase (MTHFR) piglets

Corresponding Author(s): CHEN Daiwen,Email:daiwenc@yahoo.com

Issue Date: 05 December 2010

Cite this article:

Guangbo YANG,Jingbo LIU,Daiwen CHEN, et al. Effects of folic acid supplementation on growth performance and hepatic folate metabolism-related gene expressions in weaned piglets[J]. Front Agric Chin, 2010, 4(4): 494-500.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1047-1
https://academic.hep.com.cn/fag/EN/Y2010/V4/I4/494

Tab.1 Composition and nutrient levels of basal diets (air-dry basis, %)

Tab.2 Primers used for PCR and nested PCR for detection of , , and position on cDNA

Tab.3 Effects of different levels of dietary folic acid on growth performance of weaned pigs

Tab.4 Effects of levels of folate on serum biochemical indicators of weanling pigs

Fig.1 Serum folic acid concentrations in piglets in response to different folate supplemental levels

Fig.2 Serum homocysteine concentrations in piglets in response to different folate supplemental levels

Fig.3 Effect of folate supplemental levels on the relative expression of gene in livers of piglets
Note: The transcript expression level of was higher (<0.05) in FS 0.5 and FS 2.5 than C. Data are presented in mean±SD. Different small letters denote statistical differences between groups (<0.05).

Fig.4 Effect of folate supplemental levels on the relative expression of gene in livers of piglets
Note: Higher (<0.01) expression was observed in FS 0.5, FS 2.5, and FS 5.0 than C and FS 10.0. Data are presented in mean±SD. Different small letters denote statistical differences between groups (<0.05).

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