Effects of chromosome substitution on the utilization efficiency of nitrogen, phosphorus, and potassium in wheat

doi:10.1007/s11703-011-1069-3

Front Agric Chin

2011, Vol. 5

Issue (3) : 253-261 https://doi.org/10.1007/s11703-011-1069-3

RESEARCH ARTICLE

Effects of chromosome substitution on the utilization efficiency of nitrogen, phosphorus, and potassium in wheat

Chengjin GUO¹, Jincai LI², Wensuo CHANG¹, Lijun ZHANG¹, Xirong CUI¹, Shuwen LI³, Kai XIAO¹(

)

1. College of Agronomy, Agricultural University of Hebei, Baoding 071001, China; 2. Administration Office of Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 3. College of Resource and Environment, Agricultural University of Hebei, Baoding 071001, China

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Abstract

A complete set of chromosome substitution lines with genetic background of Chinese Spring (CS) were used to determine the effects of each chromosome on utilization efficiencies of nitrogen, phosphorus, and potassium in wheat (Triticum aestivum L.). In each line, only one pair of chromosomes in CS genome was substituted by the corresponding one of donor Synthetic 6x. Under normal growth conditions supplied with enough inorganic nutrients, the dry mass per plant and the utilization efficiencies of nitrogen (N), phosphorus (P), and potassium (K) in plants varied largely among CS, Synthetic 6x, and the chromosome substitution lines (1A–7A, 1B–7B, and 1D–7D). Of these, 1A substituted by the chromosome 1A of Synthetic 6x (other lines are the same as 1A hereafter) had the highest plant dry mass and the accumulative amount of N and K, and 1B behaved to have the highest plant accumulative P amount. 1D and 4D had the lowest accumulative P amount and plant dry mass, respectively. 4B showed the lowest plant accumulative N and K. Thus, chromosome 1A of Synthetic 6x contains major genes endowing plant capacities of higher dry mass, accumulative N and K, whereas chromosome 1B of Synthetic 6x carries major genes improving plant accumulative P capacities. The lines, together with CS and the donor, could be classified into three groups including high-efficiency, mid-efficiency, and low-efficiency based on plant dry mass. Regression analysis suggested that there are significantly positive correlations between plant dry mass and the accumulated amount of N, P, and K. Further, there are positively significant correlations among the plant accumulative N amount and some plant traits and physiological parameters, as well as positively significant correlations between the accumulative amount of P and K and the photosynthetic rate (P_n).

Keywords wheat (Triticum aestivum L.) chromosome substitution line nitrogen efficiency phosphorus efficiency potassium efficiency plant growth trait photosynthetic parameter

Corresponding Author(s): XIAO Kai,Email:xiaokai@hebau.edu.cn

Issue Date: 05 September 2011

Cite this article:

Chengjin GUO,Jincai LI,Wensuo CHANG, et al. Effects of chromosome substitution on the utilization efficiency of nitrogen, phosphorus, and potassium in wheat[J]. Front Agric Chin, 2011, 5(3): 253-261.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1069-3
https://academic.hep.com.cn/fag/EN/Y2011/V5/I3/253

Fig.1 The dry mass and accumulated amount of N, P, and K in plants of CSLs, CS, and Synthetic 6x. Chinese Spring (CS) and Synthetic 6x are used as the recipient and donor of chromosomes, respectively. 1A to 7A, 1B to 7B, and 1D to 7D are chromosome substitution lines (CSLs) with just only a single pair of chromosome in CS substituted by the corresponding one of Synthetic 6x. Fig. 1A is the plant dry mass of CS, Synthetic 6x, and CSLs. The data shown are average±standard errors (). Fig. 1B is the plant accumulative N of CSLs, CS, and Synthetic 6x. Fig. 1C is the plant accumulative P of CSLs, CS, and Synthetic 6x. Fig. 1D is the plant accumulative K of CSLs, CS, and Synthetic 6x.

Fig.2 Regression analysis of plant accumulative amount of N, P, and K based on plant dry mass and nutrient utilization traits derived from CSLs, CS, and Synthetic 6x. A, D, and G show the results of regression analyses between plant accumulative N amount and plant dry mass (A), N content (D), and N efficiency (G), respectively. B, E, and H show the results of regression analyses between plant accumulative P amount and plant dry mass (B), P content (E), and P efficiency (H), respectively. C, F, and I show the results of regression analyses between plant accumulative K amount and plant dry mass (C), K content (F), and K efficiency (I), respectively. * and ** indicate that the regression coefficient reaches significant levels of 5% and 1%, respectively.

Lines	Dry mass (mg per plant)	N content (%)	P content (%)	K content (%)	Accumulative N (mg per plant)	Accumulative P (mg per plant)	Accumulative K (mg per plant)	N efficiency (mg per mg N)	P efficiency (mg per mg P)	K efficiency (mg per mg K)
CS	89a	5.64b	0.89bc	0.78a	5.02a	0.79b	0.70a	17.73a	112.66b	127.14c
Synthetic 6x	68b	6.47a	1.91a	0.69b	4.40b	1.29a	0.46b	15.45b	52.71c	147.83ab
1A	84	5.71	1.01	0.75	4.80	0.84	0.63	17.50	100.00	133.33
2A	72	6.05	0.90	0.68	4.35	0.64	0.49	16.55	112.50	146.94
3A	72	5.48	0.97	0.69	3.94	0.70	0.50	18.27	102.86	144.00
4A	59	5.48	0.92	0.66	3.23	0.54	0.39	18.27	109.26	151.28
5A	64	5.75	0.89	0.73	3.68	0.57	0.46	17.39	112.28	139.13
6A	62	5.59	1.08	0.73	3.46	0.67	0.45	17.92	92.54	137.78
7A	71	5.46	1.01	0.75	3.87	0.71	0.53	18.35	100.00	133.96
1B	71	5.45	1.49	0.75	3.87	1.06	0.53	18.35	66.98	133.96
2B	79	4.97	1.08	0.68	3.93	0.85	0.54	20.10	92.94	146.30
3B	75	5.54	0.95	0.69	4.15	0.71	0.52	18.07	105.63	144.23
4B	57	4.97	0.85	0.64	2.83	0.48	0.36	20.14	118.75	158.33
5B	62	5.8	0.79	0.67	3.59	0.49	0.41	17.27	126.53	151.22
6B	73	5.43	0.85	0.71	3.96	0.62	0.52	18.43	117.74	140.38
7B	64	5.79	0.84	0.72	3.7	0.53	0.46	17.30	120.75	139.13
1D	64	5.79	0.59	0.72	3.71	0.37	0.46	17.25	172.97	139.13
2D	65	4.87	0.84	0.67	3.1685	0.545	0.43	20.51	119.27	151.16
3D	62	5.27	0.88	0.70	3.27	0.54	0.43	18.96	114.81	144.19
4D	54	5.39	0.84	0.59	2.91	0.45	0.31	18.56	120.00	174.19
5D	75	5.65	0.78	0.67	4.23	0.59	0.50	17.73	127.12	150.00
6D	82	5.64	0.80	0.65	4.63	0.65	0.53	17.71	126.15	154.72
7D	75	5.56	0.83	0.65	4.17	0.62	0.49	17.99	120.97	153.06
A-D Ave	68.67b	5.51b	0.91bc	0.69b	3.78c	0.63b	0.47b	18.22a	113.34b	146.02ab
A-D SE	8.21	0.30	0.17	0.04	0.52	0.15	0.07	1.01	19.84	9.68
A-DCV (%)	11.96	5.38	18.87	6.00	13.77	24.64	14.96	5.57	17.50	6.63
A Ave	69.14b	5.65b	0.97b	0.71b	3.90c	0.67b	0.49b	17.75a	104.20b	140.92b
A SE	8.38	0.21	0.07	0.04	0.53	0.10	0.07	0.65	7.45	6.73
A CV (%)	12.11	3.76	7.19	5.04	13.66	14.88	15.22	3.67	7.15	4.78
B Ave	68.71b	5.42b	0.98b	0.69b	3.72c	0.68b	0.48b	18.52a	107.05b	144.79ab
B SE	7.89	0.34	0.25	0.04	0.43	0.21	0.07	1.19	20.92	8.12
B CV (%)	11.48	6.31	25.05	5.18	11.61	31.69	14.56	6.40	19.54	5.61
D Ave	68.14b	5.45b	0.79c	0.66b	3.73c	0.54c	0.45b	18.39a	128.76a	152.35a
D SE	9.58	0.31	0.10	0.04	0.64	0.10	0.07	1.10	19.94	11.04
D CV (%)	14.06	5.68	12.03	6.26	17.16	18.28	15.97	5.97	15.49	7.25

Tab.1 Plant day masses and nutrient utilization traits of the CSLs, CS, and Synthetic 6x

Lines	Plant height (cm)	Leaf age	Root number	Leaf area (cm² per plant)	SP (mg/g FW)	Chla (mg/g FW)	Chlb (mg/g FW)	Chla+Chlb (mg/g FW)	Caro (mg/g FW)	Fv/Fm	P_n (μmol/(m²·s)
CS	33.1a	3.6a	4.8a	22.6a	49.48b	1.36a	0.40a	1.76a	0.29a	0.861a	21.03a
Synthetic 6x	26.1b	3.2b	3.7c	15.1c	58.90a	1.39a	0.37a	1.76a	0.27a	0.855a	16.12b
1A	31.7	3.7	4	19.1	46.79	1.3	0.35	1.65	0.26	0.851	17.67
2A	33.5	3.5	4.2	19.3	46.62	1.39	0.37	1.76	0.29	0.852	22
3A	34.8	3.8	4.3	22.1	48.69	1.4	0.36	1.76	0.27	0.856	17.17
4A	31.2	3.6	3.8	18.9	49.72	1.43	0.4	1.83	0.31	0.847	18.84
5A	31.7	3.7	3.2	19.6	39.99	1.32	0.35	1.67	0.26	0.869	17.17
6A	30.5	3.6	3.2	17.6	34.94	1.29	0.41	1.7	0.29	0.843	16.03
7A	34.1	3.4	4.5	18.6	36.4	1.18	0.33	1.51	0.24	0.850	16.16
1B	32.2	3.5	4.2	20.2	46.44	1.29	0.36	1.65	0.25	0.861	17.15
2B	32.9	3.7	4.8	22.8	55.19	1.36	0.37	1.73	0.27	0.852	17.5
3B	35.8	3.7	4.8	23.0	50.11	1.42	0.38	1.8	0.29	0.874	21.03
4B	31.2	3.6	3.5	18.3	42.64	1.14	0.36	1.5	0.27	0.839	12.33
5B	29.1	3.7	1.8	16.6	52.21	1.45	0.41	1.86	0.3	0.851	18.16
6B	35.5	3.5	4.7	19.4	50.6	1.38	0.38	1.76	0.27	0.861	17.83
7B	34.2	3.4	4.2	19.4	45.98	1.35	0.36	1.71	0.27	0.851	18.33
1D	32.9	3.5	4.5	18.6	42.36	1.28	0.35	1.63	0.26	0.863	15.5
2D	32.7	3.6	3.7	19.5	50.98	1.31	0.34	1.65	0.26	0.864	13.03
3D	29.6	3.6	4	15.4	51.39	1.2	0.31	1.51	0.23	0.854	16.16
4D	30.6	3.5	3.2	16.4	44.58	1.09	0.25	1.34	0.25	0.852	18.1
5D	31.5	3.9	4.7	21.9	46.8	1.42	0.38	1.8	0.29	0.865	17.16
6D	38.9	3.5	4.5	23.9	48.05	1.47	0.4	1.87	0.28	0.862	18.33
7D	32.1	3.9	3.2	22.7	61.4	1.38	0.35	1.73	0.27	0.858	15.16
A-D Ave	32.70a	3.61a	3.95b	19.68b	47.23c	1.33a	0.36ab	1.69a	0.27a	0.856 a	17.18b
A-D Se	2.30	0.14	0.74	2.31	6.02	0.10	0.04	0.13	0.02	0.008	2.20
A-DCV (%)	7.03	5.45	18.72	11.76	12.74	7.82	9.98	7.90	7.35	0.966	12.82
A Ave	32.50a	3.61a	3.89bc	19.31b	43.31d	1.33a	0.37a	1.70a	0.27a	0.856a	17.86b
A Se	1.62	0.13	0.52	1.38	6.08	0.09	0.03	0.10	0.02	0.007	2.05
A CV (%)	5.00	5.15	13.33	7.17	14.04	6.41	7.82	6.06	8.64	0.849	11.50
B Ave	32.99a	3.59a	4.00b	19.96b	49.02b	1.34a	0.37a	1.72a	0.27a	0.853a	17.48b
B Se	2.40	0.12	1.08	2.31	4.25	0.10	0.02	0.12	0.02	0.011	2.60
B CV (%)	7.28	4.70	26.89	11.58	8.68	7.64	4.84	6.77	5.90	1.291	14.87
D Ave	32.61a	3.64a	3.97b	19.77b	49.37b	1.31a	0.34b	1.65b	0.26a	0.860a	16.21b
D Se	3.01	0.18	0.63	3.22	6.22	0.13	0.05	0.18	0.02	0.005	1.86
D CV (%)	9.22	6.86	15.77	16.27	12.59	10.07	14.41	10.90	7.52	0.596	11.46

Tab.2 Plant morphological traits and photosynthetic parameters in CSLs, CS, and Synthetic 6x

Tab.3 Regression analysis of accumulative N, P, and K, plant morphological traits and photosynthetic parameters in CSLs

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