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Identification of a gene responsible for the 60-day delay in flowering time of Arabidopsis |
Jihong XING1, Ye ZHANG1, Jing ZHANG1, Qiaoyun WENG2, Jiao JIA1, Jingao DONG1() |
1. Molecular Plant Pathology Lab, Agricultural University of Hebei, Baoding 071001, China; 2. Department of Agricultural and Forest Technology, Hebei North University, Zhangjiakou 075131, China |
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Abstract Identification of genes related to flowering-time in Arabidopsis is very important and meaningful contribution to the flowering process control. One late flowering mutant plant, which exhibits 60-day delay in flowering, was screened from Arabidopsis library of T-DNA insertion. Southern blotting was used to confirm the single copy of exogenetic T-DNA in the genome of the mutant. The flanking sequence of T-DNA insert was obtained by TAIL-PCR and then analyzed by BLAST to confirm that the insertion site locates at the sixth exon of AT2G19520.1 (FVE gene). FVE is considered as a classical flowering time gene in Arabidopsis. It is a component of the autonomous pathway that encodes AtMSI4, which is a putative retinoblastoma-associated protein. The late-flowering mutant is named as fve-4, which is similar to fve-3 of Columbia and allelic with fve-1 and fve-2 of Landsberg erecta. The fve-4 mutant’s delay of flowering was longer than that of fve-3 mutant, whose T-DNA insertion is located at the first exon of FVE gene, suggesting that the sixth exon of FVE gene may play a more important role in the control of floral transition.
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Keywords
delay of flowering
FVE gene
Arabidopsis
gene identification
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Corresponding Author(s):
DONG Jingao,Email:shmdjg@hebau.edu.cn
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Issue Date: 05 September 2011
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1 |
Ausin I, Alonso-Blanco C, Jarillo J A, Ruiz-García L, Martínez-Zapater J M (2004). Regulation of flowering time by FVE, a retinoblastoma-associated protein. Nat Genet , 36(2): 162–166 doi: 10.1038/ng1295 pmid:14745447
|
2 |
Boss P K, Bastow R M, Mylne J S, Dean C (2004). Multiple pathways in the decision to flower: enabling, promoting, and resetting. Plant Cell , 16(Supp1 l): S18–S31 doi: 10.1105/tpc.015958 pmid:15037730
|
3 |
He Y, Michaels S D, Amasino R M (2003). Regulation of flowering time by histone acetylation in Arabidopsis. Science , 302(5651): 1751–1754 doi: 10.1126/science.1091109 pmid:14593187
|
4 |
Jang S, Marchal V, Panigrahi K C S, Wenkel S, Soppe W, Deng X W, Valverde F, Coupland G (2008). Arabidopsis COP1 shapes the temporal pattern of CO accumulation conferring a photoperiodic flowering response. EMBO J , 27(8): 1277–1288 doi: 10.1038/emboj.2008.68 pmid:18388858
|
5 |
Kardailsky I, Shukla V K, Ahn J H, Dagenais N, Christensen S K, Nguyen J T, Chory J, Harrison M J, Weigel D (1999). Activation tagging of the floral inducer FT. Science , 286(5446): 1962–1965 doi: 10.1126/science.286.5446.1962 pmid:10583961
|
6 |
Kim H J, Hyun Y, Park J Y, Park M J, Park M K, Kim M D, Kim H J, Lee M H, Moon J, Lee I, Kim J (2004). A genetic link between cold responses and flowering time through FVE in Arabidopsis thaliana. Nat Genet , 36(2): 167–171 doi: 10.1038/ng1298 pmid:14745450
|
7 |
Kim S, Choi K, Park C, Hwang H J, Lee I (2006). SUPPRESSOR OF FRIGIDA4, encoding a C2H2-Type zinc finger protein, represses flowering by transcriptional activation of Arabidopsis FLOWERING LOCUS C. Plant Cell , 18(11): 2985–2998 doi: 10.1105/tpc.106.045179 pmid:17138694
|
8 |
Kole C, Quijada P, Michaels S D, Amasino R M, Osborn T C (2001). Evidence for homology of flowering-time genes VFR2 from Brassica rapa and FLC from Arabidopsis thaliana. Theor Appl Genet , 102(2–3): 425–430 doi: 10.1007/s001220051663
|
9 |
Komeda Y (2004). Genetic regulation of time to flower in Arabidopsis thaliana. Annu Rev Plant Biol , 55(1): 521–535 doi: 10.1146/annurev.arplant.55.031903.141644 pmid:15377230
|
10 |
Lee H, Suh S S, Park E, Cho E, Ahn J H, Kim S G, Lee J S, Kwon Y M, Lee I (2000). The AGAMOUS-LIKE 20 MADS domain protein integrates floral inductive pathways in Arabidopsis. Genes Dev , 14(18): 2366–2376 doi: 10.1101/gad.813600 pmid:10995392
|
11 |
Lee I, Aukerman M J, Gore S L, Lohman K N, Michaels S D, Weaver L M, John M C, Feldmann K A, Amasino R M (1994). Isolation of LUMINIDEPENDENS: a gene involved in the control of flowering time in Arabidopsis. Plant Cell , 6(1): 75–83 pmid:7907507
|
12 |
Liu Y G, Mitsukawa N, Oosumi T, Whittier R F (1995). Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J , 8(3): 457–463 doi: 10.1046/j.1365-313X.1995.08030457.x pmid:7550382
|
13 |
Nyathi Y, De Marcos Lousa C, van Roermund C W, Wanders R J, Johnson B, Baldwin S A, Theodoulou F L, Baker A (2010). The Arabidopsis peroxisomal ABC transporter, comatose, complements the Saccharomyces cerevisiae pxa1 pxa2 Δ mutant for metabolism of long-chain fatty acids and exhibits fatty acyl-CoA-stimulated ATPase activity. J Biol Chem , 285(39): 29892–29902
|
14 |
Onouchi H, Ige?o M I, Périlleux C, Graves K, Coupland G (2000). Mutagenesis of plants overexpressing CONSTANS demonstrates novel interactions among Arabidopsis flowering-time genes. Plant Cell , 12(6): 885–900 pmid:10852935
|
15 |
Quesada V, Macknight R, Dean C, Simpson G G (2003). Autoregulation of FCA pre-mRNA processing controls Arabidopsis flowering time. EMBO J , 22(12): 3142–3152 doi: 10.1093/emboj/cdg305 pmid:12805228
|
16 |
Schwartz C, Balasubramanian S, Warthmann N, Michael T P, Lempe J, Sureshkumar S, Kobayashi Y, Maloof J N, Borevitz J O, Chory J, Weigel D (2009). Cis-regulatory changes at FLOWERING LOCUS T mediate natural variation in flowering responses of Arabidopsis thaliana. Genetics , 183(2): 723–732 doi: 10.1534/genetics.109.104984 pmid:19652183
|
17 |
Simpson G G (2004). The autonomous pathway: epigenetic and post-transcriptional gene regulation in the control of Arabidopsis flowering time. Curr Opin Plant Biol , 7(5): 570–574 doi: 10.1016/j.pbi.2004.07.002 pmid:15337100
|
18 |
Simpson G G, Dean C (2002). Arabidopsis, the Rosetta stone of flowering time? Science , 296(5566): 285–289 doi: 10.1126/science.296.5566.285 pmid:11951029
|
19 |
Simpson G G, Dijkwel P P, Quesada V, Henderson I, Dean C (2003). FY is an RNA 3? end-processing factor that interacts with FCA to control the Arabidopsis floral transition. Cell , 113(6): 777–787 doi: 10.1016/S0092-8674(03)00425-2 pmid:12809608
|
20 |
Tadege M, Sheldon C C, Helliwell C A, Stoutjesdijk P, Dennis E S, Peacock W J (2001). Control of flowering time by FLC orthologues in Brassica napus. Plant J , 28(5): 545–553 doi: 10.1046/j.1365-313X.2001.01182.x pmid:11849594
|
21 |
Waters B M, Chu H-H, DiDonato R J, Roberts L A, Eisley R B, Lahner B, Salt D E, Walker E L (2006). Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiol , 141(4): 1446–1458 .
|
22 |
Weigel D, Alvarez J, Smyth D R, Yanofsky M F, Meyerowitz E M (1992). LEAFY controls floral meristem identity in Arabidopsis. Cell , 69(5): 843–859 doi: 10.1016/0092-8674(92)90295-N pmid:1350515
|
23 |
Yan Z Q, Liang D W, Liu H, Zheng G C (2010). FLC: A key regulator of flowering time in Arabidopsis. Russ J Plant Physiol , 57(2): 166–174 doi: 10.1134/S1021443710020020
|
24 |
Zhang J, Xu J X, Kong Y Z, Ji Z D, Wang X C, An F Y, Li C, Sun J Q, Zhang S Z, Yang X H, Mu J Y, Liu X F, Li J Y, Xue Y B, Zuo J R (2005). Generation of chemical-inducible activation tagging T-DNA insertion lines of Arabidopsis thaliana. Acta Genetica Sinica , 32(10): 1082–1088 (in Chinese) pmid:16252704
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