Studies on isolated microspore embryoid induction of <italic>C. annuum</italic> L.

doi:10.1007/s11703-010-1028-4

Front Agric Chin

2010, Vol. 4

Issue (4) : 438-442 https://doi.org/10.1007/s11703-010-1028-4

RESEARCH ARTICLE

Studies on isolated microspore embryoid induction of C. annuum L.

Ting YIN, Song TIAN, Shuangxia LUO, Xueping CHEN, Yanhua WANG, Shuxing SHEN(

)

College of Horticulture, Agricultural University of Hebei, Baoding 071001, China

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Abstract

A system to improve isolated microspore embryoid induction rate of pepper (Capsicum annuum L.) was studied in this paper. The results showed that low-temperature pretreatment, growth regulators combinations, activated carbon concentrations, and preculture temperatures were critical factors affecting embryoid formation of isolated pepper microspores in vitro. One day after pretreatment of the buds at 4°C, the anthers that differentiated and developed into embryos were cultured in double-layer medium system for one week at 7°C and then went on culturing at 28°C in darkness. All the seven genotypes of the tested pepper responded to this protocol. The embryoid induction rate of the best genotype increased from 81.11% to 147.22%. This protocol can be used as a potential tool for producing doubled haploid plants for pepper breeding.

Keywords Capsicum annuum L. isolated-microspore culture embryoids embryoid induction rate double-layer medium system

Corresponding Author(s): SHEN Shuxing,Email:shensx@hebau.edu.cn

Issue Date: 05 December 2010

Cite this article:

Ting YIN,Song TIAN,Shuangxia LUO, et al. Studies on isolated microspore embryoid induction of C. annuum L.[J]. Front Agric Chin, 2010, 4(4): 438-442.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-010-1028-4
https://academic.hep.com.cn/fag/EN/Y2010/V4/I4/438

Tab.1 Combinations of growth regulators

Fig.1 Time-course of events during isolated microspore culture of pepper
Note: (a): late-unicellular microspores; (b): cell clusters after 3 weeks of microspore culture; (c) and (d): categories of embryos obtained from microspore culture after 7 weeks; (e) and (f): differentiated embryoid of microspore culture; (g) and (h): regenerated plantlets.

Tab.2 Embryos induced from different genotypes under the same cultural conditions

Tab.3 Effect of low-temperature pretreatment of the flower buds for the embryoids induction rate

Tab.4 Effect of preculture temperature during the first week and the combinations and concentrations of growth regulators on embryoids induction rate

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