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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2007, Vol. 1 Issue (1) : 47-51    https://doi.org/10.1007/s11703-007-0008-9
Research article
Active oxygen metabolism in the floral buds and leaves of the new cytoplasm male sterile (CMS) line and its maintainer line of non-heading Chinese cabbage
Jianyu YUAN,Xilin HOU(),Changwei ZHANG,Fan YE
National Key Laboratory of Crop Genetics & Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
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Abstract

The cytoplasm male sterile (CMS) line and its maintainer line of the newly-bred non-heading Chinese cabbage were taken as experimental materials. The production rate of superoxide anion radical (O2- ), contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) as well as ascorbate peroxidase (APX) were determined in buds of different developmental stages and leaves during vegetative and florescence stages. As showed by the results, there were higher levels of MDA and H2O2 contents, higher O2- production rate and higher activities of SOD, POD, and CAT in the buds of the CMS lines than that in the buds of the fertile lines, while the APX activity was different from the other three enzymes above. There were differences in activities of protective enzymes in leaves but no significant differences in MDA contents and O2- production rate, which showed that the differences in metabolism of active oxygen between CMS and its maintainer were mainly apparent in reproductive organs.

Keywords non-heading Chinese cabbage      cytoplasm male sterility (CMS)      lipid peroxdation      active oxygen metabolism      protective enzymes     
Issue Date: 22 February 2016
 Cite this article:   
Jianyu YUAN,Xilin HOU,Changwei ZHANG, et al. Active oxygen metabolism in the floral buds and leaves of the new cytoplasm male sterile (CMS) line and its maintainer line of non-heading Chinese cabbage[J]. Front. Agric. China, 2007, 1(1): 47-51.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0008-9
https://academic.hep.com.cn/fag/EN/Y2007/V1/I1/47
Fig. 1  Comparison of O2- generating rate, H2O2 and MDA contents in floral buds of new CMS line with its maintainer line during different developmental stages in non-heading Chinese cabbage
Fig. 2  Comparison of activities of POD(A), SOD(B), CAT(C) and APX(D) in floral buds of new CMS line and its maintainer line during different developmental stages in non-heading Chinese cabbage
Stage Line O2- prodution rate
/μmol · g-1 · min-1
H2O2 contents
/nmol · g-1
MDA contents
/nmol · g-1
Activity of enzymes /ΔOD · g-1 · min-1
POD SOD CAT APX
Vegetative stage MF 0.51aA 6.40aA 2.52aA 4540.51aA 131.91bB 3578.18bA 5864.14aA
MS 0.40aA 5.30aA 2.46aA 3458.34bB 181.42aA 3718.27aA 5717.79aA
Flowering stage MF 0.29aA 40.57aA 43.9bA 2534.21bA 108.81bA 2464.30bA 1876.51aA
MS 0.250aA 41.56aA 49.6aA 3281.41aA 132.21aA 2950.10aA 1978.41aA
Table 1   O 2 - production rate, H2O2 contents, MDA contents and activities of POD, SOD, CAT and APX in leaves during different developmental stages in non-heading Chinese cabbage
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