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Generation and identification of four new monosomic addition lines of flowering Chinese cabbage-Chinese kale |
Erqiao SHEN, Xiaofeng LI, Chenghe ZHANG( ), Xine WANG, Shuxin XUAN |
| College of Horticulture, Agricultural University of Hebei, Baoding 071001, China |
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Abstract A selection test of four new flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee)-Chinese kale (Brassica alboglabra Bailey) monosomic alien addition lines was conducted by karyotype analysis and morphological marker identification. The results mainly showed the following: 61‘2n + 1’ plants screened out from 655 progeny plants of the trigenomic hybrid (AAC) backcrossed with its parent flowering Chinese cabbage (AA), four new monosomic alien addition lines(AA+ C1, AA+ C5, AA+ C8 and AA+ C9) obtained from the 61 ‘2n + 1’ plants, the transmission rates of the monosomic alien addition lines (AA+ C1, AA+ C5 and AA+ C8) of 19.7%, 23.44%, and 36.51% by female gametes and 7.84%, 8.89%, and 9.43% by male gametes, respectively, and the alien chromosome C-1 and C-5 had partial homology with at least one chromosome of A-genome.
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| Keywords
flowering Chinese cabbage (Brassica campestris L. var. utilis Tsen et Lee)
Chinese kale (Brassica alboglabra Bailey)
monosnomic alien addition lines
transmission rate
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Corresponding Author(s):
ZHANG Chenghe,Email:zhangchenghe06@126.com
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Issue Date: 05 June 2011
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| 1 |
Barthes L, Ricroh A (2001). Interspecific chromosomal rearrangements in monosomic addition lines of Allium. Genome , 44(5): 929–935
doi: 10.1139/gen-44-5-929
|
| 2 |
Biyashev R M, Ragab R A, Maughan P J (1997). Molecular mapping, chromosomal assignment and genetic diversity analysis of phytochrome loci in barley. Heredity , 88: 21–26
|
| 3 |
Chen B Y, Cheng B F, Jorgensen R B, Heneen W K (1997). Production and cytogenetics of Brassica campestris-alboglabra chromosome addition lines. Theor Appl Genet , 94(5): 633–640
doi: 10.1007/s001220050460
|
| 4 |
Chen B Y, Simonsen V, Lanner-Herrera C, Heneen W K (1992). A Brassica campestris-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomics. Theor Appl Genet , 84: 592–599
doi: 10.1007/BF00224157
|
| 5 |
Cheng B F, Chen B Y, Heneen W K (1994). Addition of Brassica alboglabra Bailey chromosomes to B. campestris L. with special emphasis on seed color. Heredity , 73(2): 185–189
doi: 10.1038/hdy.1994.118
|
| 6 |
Chetelat R T, Rick C M, Cisneros P, Alpert K B, DeVerna J W (1998). Identification, transmission, and cytological behavior of Solanum lycopersicoides Dun. monosomic alien addition lines in tomato (Lycopersicon esculentum Mill.). Genome , 41(1): 40–50
doi: 10.1139/gen-41-1-40
|
| 7 |
Chèvre A M, Eber F, Barret P, Dupuy P, Brace J (1997). Identification of the different Brassica nigra chromosome from both sets of B. oleracea-B. nigra and B. napus-B. nigra addition lines with a special emphasis on chromosome transmission and self-incompathibility. Theor Appl Genet , 94(5): 603–611
doi: 10.1007/s001220050457
|
| 8 |
Hasterok R, Wolny E, Kulak S, Zdziechiewicz A, Maluszynska J, Heneen W K (2005). Molecular cytogenetic analysis of Brassica rapa×Brassica oleracea var. alboglabra monosomic addition lines. Theor Appl Genet , 111(2): 196–205
doi: 10.1007/s00122-005-1942-7
|
| 9 |
Jahier J, Chevre A M, Tanguy A M, Eber F (1989). Extraction of disomic addition lines of Brassica napus-B. nigra. Genome , 32: 408–413
|
| 10 |
Li M X, Zhang Z P (1996). Crop Chromosome And Manipulation Technique. Beijing: China Agricultural Press, 17–19 (in Chinese)
|
| 11 |
Liu W, Shen S X, Wang Y H, Zhang C H, Xuan S X (2008). A study on obtaining and identification of Chinese cabbage-cabbage addition lines. Acta Horticulturae Sinica , 35(2): 207–212 (in Chinese)
|
| 12 |
Man H, Zhang C H, Wang X E, Zhang G H (2007). Obtaining and identification of the allotetraploid from 4x Caitai 9601×4x Jielan 9705. Acta Horticulturae Sinica , 34(5): 1163–1168 (in Chinese)
|
| 13 |
McGrath J M, Quiros C F, Harada J J, Landry B S (1990). Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet , 223(2): 198–204
doi: 10.1007/BF00265054
|
| 14 |
Quiros C F, Ochoa O, Kianian S F, Douches D (1987). Analysis of the Brassica. alboglabra genome by the generation of B. campestris-B. alercea addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet , 74(6): 758–766
doi: 10.1007/BF00247554
|
| 15 |
Wang X E, Zhang C H, Xuan S X, Man H, Liu H H, Shen S (2008). Monosomic addition lines of flowering Chinese cabbage (B. campestris Lssp. Chinensis var. parachinensis L. H. Bailey) -Chinese kale (B.oleracea L. var. alboglabra L. H. Bailey). Agric Sci China , 7(6): 656–663
doi: 10.1016/S1671-2927(08)60099-1
|
| 16 |
Wang Y P, Sonntag K, Rudloff E, Wihling P, Snowdon R J (2006). GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines. Plant Cell Rep , 25(1): 35–40
doi: 10.1007/s00299-005-0031-3
|
| 17 |
Yuan H, Zhang C H, Man H, Wang X E (2008). Embryo development observation of 4x flowering Chinese cabbage-2x Chinese kale and obtaining of the allotriploid by ovary culture. Journal of Plant Genetic Resources , 9(3): 297–301 (in Chinese)
|
| 18 |
Zhu J S, Struss D, Robbelen G (1993). Studies on resistance to phoma lingam in Brassica napus-Brassica nigra addition lines. Plant Breed , 111(3): 192–197
doi: 10.1111/j.1439-0523.1993.tb00629.x
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