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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front Agric Chin    2011, Vol. 5 Issue (3) : 284-290    https://doi.org/10.1007/s11703-011-1075-5
RESEARCH ARTICLE
Comparative mapping of QTLs for H+ secretion of root in maize (Zea mays L.) and cross phosphorus levels on two growth stages
Junyi CHEN(), Li XU
Institute of Medical Biotechnology in Chongqing/Institute of Chinese Medicine in Chongqing, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China
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Abstract

H+ is a root secretion that affects P acquisition and P-use efficiency (PUE) under deficient phosphorus in maize. The secretion of H+, difference value of H+ between deficient and normal phosphorus (DH), and relative H+ (RH) as well as the quantitative trait loci (QTLs) associated with these traits were determined for a F2:3 population derived from the cross of two contrasting maize (BoldItalic L.) genotypes, 082 and Ye107. By using composite interval mapping (CIM), a total of 14, 8, and 9 distinct QTLs were identified for H+, DH, and RH, respectively. Most loci of QTLs for traits H+, DH, and RH had different cross environments. It showed that H+ secretion possessed an environment-sensitive and multi-gene nature. The gene × environment interaction was actually reflected by H+ secretion. One region for QTL of trait H+ was detected at the interval of bnlg2228-bnlg100 (bin 1.08) on chromosome 1. Coincident QTLs in the important genomic region reflected the cross phosphorus levels, different cross growth stages, and two different cross environments. The QTL explained 10% to 14% total phenotypic variance of H+. Therefore, the above segment (bnlg2228-bnlg100) (bin 1.08) identified on chromosome 1 may be used in the future for MAS to improve the phosphorus efficiency in maize.

Keywords maize      QTL analysis      H+      difference value of H+      relative H+     
Corresponding Author(s): CHEN Junyi,Email:chenjunyi3@126.com   
Issue Date: 05 September 2011
 Cite this article:   
Junyi CHEN,Li XU. Comparative mapping of QTLs for H+ secretion of root in maize (Zea mays L.) and cross phosphorus levels on two growth stages[J]. Front Agric Chin, 2011, 5(3): 284-290.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-011-1075-5
https://academic.hep.com.cn/fag/EN/Y2011/V5/I3/284
TraitsParentsF2∶3 families
082Ye107MeanRangeσ^G2σ^E2hb2(%)
H-KXDP0.06700.03300.05250.0240-0.08100.00020.000161.3
H-KXNP0.02230.01990.02260.0114-0.04550.00010.000167.5
H-SUDP10.06900.03800.05760.0250-0.09200.00020.000163.1
H-SUDP20.08140.06780.06750.0325-0.11480.00030.000265.7
DH-KX0.04470.01310.02990.0126-0.05410.00010.000155.7
DH-SU10.0379-0.00600.0233-0.0113-0.04910.00010.000158.9
DH-SU20.03810.01270.03080.0108-0.06180.00010.000164.7
RH-KX3.00201.65602.37803.0570-1.49900.15100.140051.9
RH-SU12.22000.86301.76002.2300-0.86400.08500.083050.2
RH-SU21.88001.23001.91471.2480-2.52000.08380.073153.4
Tab.1  Estimates of genetic variance and environment variance among 241 F families from the cross of 082 × Ye107
TraitsParents
082Ye107
PEa0.8030.548
PAEa3.2302.237
WPUE-NPa5.246.01
WPUE-NPa5.115.87
H-DPa0.06700.0330
H-NPa0.02230.0199
Tab.2  Phosphorus traits of 082 × Ye107
TreatmentsSource of variationSSDegree of freedomF value
SitesGenotypes0.08882402.14**
Environments0.003114.56**
G × E--3.54**
P levelsGenotypes0.03912401.59**
Environments0.107612.17*
G × E--4.55**
StagesGenotypes0.11162400.673*
Environments0.011914.23**
G × E--0.412**
Tab.3  value of ANOVA for effects on H
TreatmentsSource of variationSSDegree of freedomF value
SitesGenotypes0.03392403.05**
Environments0.00521112.84**
G × E--24.67*
StagesGenotypes0.03502406.72**
Environments0.00671310.60**
G × E--43.26*
Tab.4  value of ANOVA for effects on DH
TreatmentsSources of variationSSDegree of freedomF value
SitesGenotypes36.532401.80**
Environments45.911543.99**
G × E36.65*
StagesGenotypes33.782404.96**
Environments2.871101.34**
G × E37.85*
Tab.5  value of ANOVA for effects on RH
ItemPUEH+DH
H+0.7374**
DH0.7905**0.4028
RH0.7033**0.41290.4046
Tab.6  Analyze of correlation among PUE, H, DH, and RH
NameCQPInterval markersClosest markersBinsLODTR2 (%)ADGADir
H1-KXDP1146.7bnlg2228-bnlg100bnlg1001.082.8313-0.0027-0.002PDYe107
H2a-KXDP232.1phi098-umc1165phi0982.023.45140.00090.0056ODYe107
H2b-KXDP246.0umc1185-umc1555umc11852.032.8422-0.00650.0052PDYe107
H8-KXDP879.0P3M7/b-dupssr14dupssr148.092.9516-0.0021-0.0024DYe107
H1-SUDP11146.7bnlg2228-bnlg100bnlg1001.082.8612-0.0026-0.0032ODYe107
H1-SUDP21146.7bnlg2228-bnlg100bnlg1001.082.9810-0.00190.0074ODYe107
H1-KXNP1146.7bnlg2228-bnlg100bnlg1001.083.1611-0.02170.0276ODYe107
H5-SUDP2588.7umc1092-phi331888umc10925.042.86120.0522-0.0530OD082
H5a-SUDP2516.0bnlg1006-P2M5/dP2M5/d5.002.6915-0.00570.0051DYe107
H3-KXNP349.7bnlg1325b-umc1772bnlg1325b3.022.93120.0027-0.0023D082
H5b-KXNP588.7umc1092-phi331888umc10925.042.50100.0020-0.0027OD082
H10-KXNP10154.2P2M8/a-bnlg1839bnlg183910.073.397-0.00100.0106ODYe107
DH4-KX4121.6P4M3/g-bnlg1337bnlg13374.102.60150.0013-0.0047OD082
DH5-KX516.0bnlg1006-P2M5/dP2M5/d5.003.3416-0.00340.0022PDYe107
DH8-KX879.0P3M7/b-dupssr14dupssr148.093.5911-0.0015-0.0022ODYe107
DH1-SUDP11116.0bnlg1556-bnlg1564bnlg15561.072.7210-0.00150.0044ODYe107
DH9a-SUDP1968.7nc134-P1M3/fP1M3/f9.032.5417-0.00460.0055DYe107
DH9b-SUDP1986.6P1M3/d-P1M3/gP1M3/g9.043.3870.00170.0047OD082
DH9c-SUDP19104.4umc1094-bnlg1191bnlg11919.062.6811-0.00240.0062ODYe107
DH6-SUDP2651.4umc1918-umc2316umc19186.033.23190.0039-0.0023PD082
RH6-KX636.4umc1572-phi389203umc15726.023.519-0.16870.1135PDYe107
RH2-SUDP1295.4nc131-P5M1/anc1312.052.906-0.10000.1068DYe107
RH3-SUDP13207.4umc1273-P1M4/iumc12733.084.7510.0096-0.1437OD082
RH8-SUDP180.0bnlg1252-umc1075bnlg12528.003.083-0.06910.1339ODYe107
RH1-SUDP2193.5bnlg1023-bnlg1041bnlg10231.063.04230.1215-0.0487PD082
RH3a-SUDP2349.7bnlg1325b-umc1772bnlg1325b3.022.6419-0.10930.0566PDYe107
RH3b-SUDP23207.4umc1273-P1M4/iumc12733.082.67140.0093-0.1037OD082
RH5a-SUDP25111.1dupssr10-umc1110bdupssr105.043.2519-0.13060.0463PDYe107
RH5b-SUDP25145.9umc1155-umc1264umc11555.053.00140.07950.0315PD082
Tab.7  QTLs detected for H, DH, and RH with the F families from the cross of 082 × Ye107
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