|
|
Molecular cloning and sequence analysis of an LFY homologous gene from Juglans regia L. |
Fuqiang HE, Hongxia WANG, Zhihua ZHANG() |
Mountainous Areas Research Institute, Agricultural University of Hebei, Baoding 071001, China |
|
|
Abstract The existence of a long juvenile phase is found seriously to affect the early-stage economic benefits of later mature walnuts (Juglans regia L.). Studies on LFY, a key gene controlling floral transition and flower differentiation, may be helpful in solving the problem. This study reports the identification and characterization of a JrLFY gene from Juglans regia L., a homolog of FLORICAULA/LFY. The gene was isolated from flower buds of precocious walnut cultivar Zhonglin No. 5 by RT-PCR and RACE. The cDNA sequence of JrLFY (GenBank accession no. GU194836) was 1496 bp and contained an ORF of 1158 bp. Its corresponding genomic sequence (GenBank accession no. HQ019159) showed that the JrLFY contained three exons and two introns. The predicted amino acid sequence of the gene consisted of 385 amino acids and had a conserved region in the C-terminal when being aligned with sequences of other LFY homologs. Phylogenetic analysis showed that the LFY protein of walnut was close to those of hickory and chestnut. These studies will lay a foundation for understanding the mechanism of early fruiting and preparation for transfer of the JrLFY as a transgene to later mature walnuts.
|
Keywords
walnut
JrLFY
early mature
precocious
juvenility
|
Corresponding Author(s):
ZHANG Zhihua,Email:shylg@hebau.edu.cn
|
Issue Date: 05 September 2011
|
|
1 |
An X M, Wang D M, Wang Z L, Li B, Bo W H, Cao G L, Zhang Z Y (2011). Isolation of a LEAFY homolog from Populus tomentosa: expression of PtLFY in P. tomentosa floral buds and PtLFY-IR-mediated gene silencing in tobacco (Nicotiana tabacum). Plant Cell Rep , 30(1): 89-100
|
2 |
Baum D A, Yoon H S, Oldham R L (2005). Molecular evolution of the transcription factor LEAFY in Brassicaceae. Mol Phylogenet Evol , 37(1): 1-14 doi: 10.1016/j.ympev.2005.07.002 pmid:16112883
|
3 |
B?urle I, Dean C (2006). The timing of developmental transitions in plants. Cell , 125(4): 655-664 doi: 10.1016/j.cell.2006.05.005 pmid:16713560
|
4 |
Benlloch R, Berbel A, Serrano-Mislata A, Madue?o F (2007). Floral initiation and inflorescence architecture: a comparative view. Ann Bot (Lond) , 100(3): 659-676 doi: 10.1093/aob/mcm146 pmid:17679690
|
5 |
Blázquez M A, Ferrándiz C, Madueáo F, Parcy F (2006). How floral meristems are built. Plant Mol Biol , 60(6): 855-870 doi: 10.1007/s11103-006-0013-z pmid:16724257
|
6 |
Bowman J L, Alvarez J, Weigel D, Meyerowitz E M, Smyth D R (1993). Control of flower development in Arabidopsis thaliana by APETALA1 and interacting genes. Development , 119: 721-743
|
7 |
Breton C, Cornu D, Chriqui D, Sauvanet A, Capelli P, Germain E, Jay-Allemand C (2004). Somatic embryogenesis, micropropagation and plant regeneration of “Early Mature” walnut trees (Juglans regia) that flower in vitro. Tree Physiol , 24(4): 425-435 pmid:14757582
|
8 |
Brunner A M, Rottmann W H, Sheppard L A, Krutovskii K, DiFazio S P, Leonardi S, Strauss S H (2000). Structure and expression of duplicate AGAMOUS orthologues in poplar. Plant Mol Biol , 44(5): 619-634 doi: 10.1023/A:1026550205851 pmid:11198423
|
9 |
Carmona M J, Cubas P, Martínez-Zapater J M (2002). VFL, the grapevine FLORICAULA/LEAFY ortholog, is expressed in meristematic regions independently of their fate. Plant Physiol , 130(1): 68-77 doi: 10.1104/pp.002428 pmid:12226487
|
10 |
Guo J L, Yang Q (2008). Molecular Cloning and expression analysis of a LFY homologous gene from potato. Plant Mol Biol Rep , 26(4): 324-334 doi: 10.1007/s11105-008-0045-4
|
11 |
Hempel F D, Weigel D, Mandel M A, Ditta G, Zambryski P C, Feldman L J, Yanofsky M F (1997). Floral determination and expression of floral regulatory genes in Arabidopsis. Development , 124(19): 3845-3853 pmid:9367440
|
12 |
Kim D H, Han M S, Cho H W, Kim D S, Kim H J, Kim B D (2008). Molecular cloning of the CaLFY, putative pepper ortholog of FLO/LFY. Mol Breeding , 22(3): 443-453 doi: 10.1007/s11032-008-9188-0
|
13 |
Kobayashi Y, Weigel D (2007). Move on up, it’s time for change—mobile signals controlling photoperiod-dependent flowering. Genes Dev , 21(19): 2371-2384 doi: 10.1101/gad.1589007 pmid:17908925
|
14 |
Mandel M A, Gustafson-Brown C, Savidge B, Yanofsky M F (1992). Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature , 360(6401): 273-277 doi: 10.1038/360273a0 pmid:1359429
|
15 |
Mandel M A, Yanofsky M F (1995). A gene triggering flower in Arabidopsis. Nature , 377: 522-524 doi: 10.1038/377522a0 pmid:7566148
|
16 |
Montieri S, Gaudio L, Aceto S (2004). Isolation of the LFY/FLO homologue in Orchis italica and evolutionary analysis in some European orchids. Gene , 333: 101-109 doi: 10.1016/j.gene.2004.02.015 pmid:15177685
|
17 |
Parcy F, Nilsson O, Busch M A, Lee I, Weigel D (1998). A genetic framework for floral patterning. Nature , 395(6702): 561-566 doi: 10.1038/26903 pmid:9783581
|
18 |
Pe?a L, Martín-Trillo M, Juárez J, Pina J A, Navarro L, Martínez-Zapater J M (2001). Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nat Biotechnol , 19(3): 263-267 doi: 10.1038/85719 pmid:11231561
|
19 |
Prusinkiewicz P, Erasmus Y, Lane B, Harder L D, Coen E (2007). Evolution and development of inflorescence architectures. Science , 316(5830): 1452-1456 doi: 10.1126/science.1140429 pmid:17525303
|
20 |
Rotem N, Shemesh E, Peretz Y, Akad F, Edelbaum O, Rabinowitch H D, Sela I, Kamenetsky R (2007). Reproductive development and phenotypic differences in garlic are associated with expression and splicing of LEAFY homologue gaLFY. J Exp Bot , 58(5): 1133-1141 doi: 10.1093/jxb/erl272 pmid:17237158
|
21 |
Sheppard L A, Brunner A M, Krutovskii K V, Rottmann W H, Skinner J S, Vollmer S S, Strauss S H (2000). A DEFICIENS homolog from the dioecious tree black cottonwood is expressed in female and male floral meristems of the two-whorled, unisexual flowers. Plant Physiol , 124(2): 627-640 doi: 10.1104/pp.124.2.627 pmid:11027713
|
22 |
Tandre K, Albert V A, Sund?s A, Engstr?m P (1995). Conifer homologues to genes that control floral development in angiosperms. Plant Mol Biol , 27(1): 69-78 7865797 doi: 10.1007/BF00019179
|
23 |
Wagner D, Sablowski R W M, Meyerowitz E M (1999). Transcriptional activation of APETALA1 by LEAFY. Science , 285(5427): 582-584 doi: 10.1126/science.285.5427.582 pmid:10417387
|
24 |
Weigel D, Alvarez J, Smyth D R, Yanofsky M F, Meyerowitz E M (1992). LEAFY controls floral meristem identity in Arabidopsis. Cell , 69(5): 843-859 doi: 10.1016/0092-8674(92)90295-N pmid:1350515
|
25 |
Weigel D, Nilsson O (1995). A developmental switch sufficient for flower initiation in diverse plants. Nature , 377(6549): 495-500 doi: 10.1038/377495a0 pmid:7566146
|
26 |
William D A, Su Y, Smith M R, Lu M, Baldwin D A, Wagner D (2004). Genomic identification of direct target of LEAFY. Proc Natl Acad Sci USA , 101(6): 1775-1780 doi: 10.1073/pnas.0307842100 pmid:14736918
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|