|
|
Isolation and characterization of Bacillus subtilis EB-28, an endophytic bacterium strain displaying biocontrol activity against Botrytis cinerea Pers. |
Shutong WANG1, Tongle HU1, Yanling JIAO1, Jianjian WEI2, Keqiang CAO1() |
1. Bio-control Center of Plant Disease and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China; 2. Beijing TEPEC Sunshine Science and Technology Cooperation, Beijing 100086, China |
|
|
Abstract The fungal pathogen Botrytis cinerea Pers. causes severe rotting on tomato fruits during storage and shelf life. As a biological control agent, endophytic bacterium was regarded as an effective alternative to chemical control. Out of 238 endophytic bacterial isolates, three strains (EB-15, EB-28, and EB-122) isolated from Lycopersicum esculentum Mill., Speranskia tuberculata (Bge.) Baill, and Dictamnus dasycarpus Turcz. respectively were found to be strongly antagonistic to the pathogen in vitro and were selected for further in vivo tests. One endophytic bacterium strain, encoded EB-28, was selected from the three in vivo tested isolates. The inhibitive rate of EB-28 reached 71.1% in vitro and 52.4% in vivo. EB-28 was identified as Bacillus subtilis according to its morphological, physiological, and biochemical characteristics and 16S rDNA sequence analysis.
|
Keywords
endophytic bacterium
Bacillus subtilis
Botrytis cinerea Pers
biological control
|
Corresponding Author(s):
CAO Keqiang,Email:ckq@hebau.edu.cn
|
Issue Date: 05 September 2009
|
|
1 |
Barritt M M (1936). The intensification of the Voges-Proskauer reaction by the addition of α-naphtol. J Pathol Bacteriol , 42: 441-445 doi: 10.1002/path.1700420212
|
2 |
Bergey's Manual of Determinative Bacteriology (1994). Bergey’s Manual of Determinative Bacteriology, 9th ed. Baltimore: The Williams and Wilkins Company
|
3 |
Chen H, Xiao X, Wang J, Wu L J, Zheng Z M, Yu Z L (2008). Antagonistic effects of volatiles generated by Bacillus subtilis on spore germination and hyphal growth of the plant pathogen, Botrytis cinerea. Biotechnological Letters , 30: 919-923 doi: 10.1007/s10529-007-9626-9
|
4 |
Clarke P H (1953). Hydrogen sulphide production by bacteria. J Gen Microbiol , 8: 397-407
|
5 |
Du C F, Yang X X, Tu P F (2005). Studies on chemical constituents in bark of Dictamnus dasycarpus. China Journal of Chinese Materia Medica , 30(21): 1663-1666 (in Chinese)
|
6 |
Elad Y, Stewart A (2004). Microbial control of Botrytis spp. In: Elad Y, Williamson B, Tudzynski P, Delen N, eds. Botrytis: Biology, Pathology and Control . The Netherlands: Kluwer Academic Press, 223-241 doi: 10.1007/978-1-4020-2626-3_13
|
7 |
Guo Y B, Zheng H, Yang Y L (2007). Characterization of Pseudomonas corrugate strain P94 isolated from soil in Beijing as a potential biocontrol agent. Curr Microbiol , (55): 247-253 doi: 10.1007/s00284-007-0120-3
|
8 |
Hacene H, Rafa F, Chebhouni N, Boutaiba S, Bhatnagar T, Baratti JC, Ollivier B (2004). Biodiversity of prokaryotic micro-flora in El Golea Salt lake Algerian Sahara. J Arid Environ , 58: 273-284 doi: 10.1016/j.jaridenv.2003.08.006
|
9 |
Hillis D M, Dixon M T (1991). Ribosomal DNA: molecular evolution and phylogenetic inference. Quart Rev Biol 66: 411-453 doi: 10.1086/417338
|
10 |
Huang G R, Du X Y, Xu D G, Zhang H, Pan R Q, Xu H H (2007). Antifungal activities in methanol extracts from twenty species of plants in three gorges region Hubei province. Journal of South China Agricultural University , 28(3): 37-41 (in Chinese)
|
11 |
Jiao Y L, Wang S T, Cao K Q, (2009). Establishment and application of bioassay method for screening fungicides against Botrytis cinerea Pers. Journal of Agricultural University of Hebei , 31(In press)(in Chinese)
|
12 |
Li C, Zhang C Z, Hu F D, Shi J G (2000). Chemical Constituents from Speranskia tuberculata (Bge.) Baill. China Journal of Chinese Materia Medica , 25(5): 291-292 (in Chinese)
|
13 |
Liu C H, Zou W X, Lu H, Tan R X (2001). Antifungal activity of Artimisia annua endophyte cultures against some phytopathogenic fungi. Journal of Biotechnology , 88: 277-282 doi: 10.1016/S0168-1656(01)00285-1
|
14 |
Liu C H, Chen X, Liu T T, Lian B, Gu Y C, Caer V, Xue Y R, Wang B T (2007). Study of the antifungal activity of Acinetobacter baumannii LCH001 in vitro and identification of its antifungal components. Applied Microbiology and Biotechnology , 76: 459-466 doi: 10.1007/s00253-007-1010-0
|
15 |
Lodewyckx C, Vangronsfeld J, Porteous F, Moore E R B, Taghavi S, Mergeay M, van der Lelie D (2002). Endophytic bacteria and their potential applications. Critical Review of Plant Science , 21: 583-606 doi: 10.1080/0735-260291044377
|
16 |
Moundt J O, Hinckle N F (1976). Bacteria within ovules and seeds. Applied and Environmental Microbiology , 32: 694-698
|
17 |
Sadifi N, Cherif M, Fliss I, Boudabous A, Antoun H (2001). Evaluation of Bacillus isolates from salty soils and Bacillusthuringiensis strains for the biocontrol of Fusarium dry rot of potato tubers. Journal of Plant Pathology , 83: 101-108
|
18 |
Sadifi N, Cherif M, Hajlaoui M R, Boudabous A (2002). Biological control of the potato tubers dry rot caused by Fusarium roseum var. sambucinum under greenhouse, field and storage conditions using Bacillus sp. isolates. Journal of Phytopathology , 150: 640-648 doi: 10.1046/j.1439-0434.2002.00811.x
|
19 |
Sadfi N, Essghaier B, Hajlaoui M R, Fardeau M L, Cayaol J L, Ollivier B, Boudabous A (2008). Ability of moderately halophilic bacteria to control grey mould disease on tomato fruits. Journal of Phytopathology , 156(1): 42-52
|
20 |
Sessitsch A, Reiter B, Berg G (2004). Endophytic bacterial communities of field-grown potato plants and their plant-growth promoting and antagonistic abilities. Canadian Journal of Microbiology , 50: 239-249 doi: 10.1139/w03-118
|
21 |
Sturz A V, Christie B R, Nowak J (2000). Bacterial endophytes: potential role in developing sustainable system of crop production. Critical Review of Plant Science , 19(1): 1-30 doi: 10.1016/S0735-2689(01)80001-0
|
22 |
Walker R, Powell A A, Seddon B (1998). Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species. Journal of Applied Microbiology , 84: 791-801 doi: 10.1046/j.1365-2672.1998.00411.x
|
23 |
Whipps J M (1987). Effect of media on growth and interactions between a range of soil-borne glasshouse pathogens and antagonistic fungi. New Phytologist , 107(1): 127-142 doi: 10.1111/j.1469-8137.1987.tb04887.x
|
24 |
Williamson B, Tudzynski B, Tudzynski P, van Kan J A L (2007). Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology , 8(5): 561-580 doi: 10.1111/j.1364-3703.2007.00417.x
|
25 |
Wu X Y, Walker M J, Hornitzky M, Chin J (2006) Development of a group-specific PCR combined with ARDRA for the identification of Bacillus species of environmental significance. J Microbiol Methods , 64: 107-119 doi: 10.1016/j.mimet.2005.04.021
|
26 |
XiaoT L, Liu W W, Fang D C (2000). Construction of phage antibody library and screening of human anti-ATP monoclonal antibody. Acta Academiae Medicinae Militaris Tertiae , 22(7): 682-684 (in Chinese)
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|