Somatic embryogenesis and plant regeneration in glandless upland cotton (<italic>Gossypium hirsutum</italic> L.)

doi:10.1007/s11703-009-0060-8

Front Agric Chin

2009, Vol. 3

Issue (3) : 279-283 https://doi.org/10.1007/s11703-009-0060-8

RESEARCH ARTICLE

Somatic embryogenesis and plant regeneration in glandless upland cotton (Gossypium hirsutum L.)

Jingyan SUN¹, Weiming LI¹(

), Hanshuang ZHANG¹, Junli ZHAO¹, Xiaolin YIN¹, Li’an WANG²

1. Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 2. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China

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Abstract

Glandless upland cotton has an important economic value. Embryogenic calli and regenerated plants were obtained from the hypocotyl explants of glandless upland cotton seedlings, cultivar Jisheng1. The results indicated that somatic embryogenesis was significantly influenced by the types of auxin and cytokinin. 2, 4-D was advantageous to induce cotton callus, but embryogenic callus could not be obtained on the 2, 4-D medium. Embryogenic calli were also not obtained on the MSB sold medium with the combination of IBA and BA. However, embryogenic calli were induced when the hypocotyl explants were cultured on the IBA and KT medium. More than 31% of the hypocotyl segments produced embryogenic calli when the MSB medium was supplemented with 1.0 mg·L^-1 IBA and 0.5 mg·L^-1 KT. Embryogenic calli with somatic embryos could be observed within three months. Somatic embryo germination and maturation occurred on the hormone-free MSB medium with 1.0 g·L^-1 Gln and 0.5 g·L^-1 Asn. A number of regenerated plants could be obtained in six months. In the present study, a simple and efficient system was established to induce a number of embryogenic calli and regenerate plantlets from hypocotyl explants.

Keywords cotton (Gossypium hirsutum L.) glandless somatic embryogenesis plant regeneration

Corresponding Author(s): LI Weiming,Email:li_wyman@yahoo.com

Issue Date: 05 September 2009

Cite this article:

Jingyan SUN,Weiming LI,Hanshuang ZHANG, et al. Somatic embryogenesis and plant regeneration in glandless upland cotton (Gossypium hirsutum L.)[J]. Front Agric Chin, 2009, 3(3): 279-283.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0060-8
https://academic.hep.com.cn/fag/EN/Y2009/V3/I3/279

Tab.1 Effects of hormone combinations and measures on the induction of embryogenic callus

Fig.1 Somatic embryogenesis of the glandless upland cotton
Note: (a)–(f) represent callus induced on the medium with the combination of 2,4-D, IAA and KT, callus induced on the medium with the combination of IBA and BA, callus induced on the medium with the combination of IBA and KT, embryogenic callus, the germination of somatic embryos and regenerated plants of Jisheng1, respectively.

Fig.2 Effect of the concentrations of the IBA and KT on the induction rate of callus and embryogenic callus

Tab.2 The effects of the concentrations of glutamine and asparagine on the germination of embryo

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