mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

doi:10.1007/s11703-007-0059-y

Front. Agric. China

2007, Vol. 1

Issue (3) : 352-356 https://doi.org/10.1007/s11703-007-0059-y

mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP

LI Xianglong¹, ZHANG Zengli¹, JIA Qing¹, WANG Lize¹, GONG Yuanfang², LIU Zhengzhu²

1.Department of Animal Science & Technology, Agricultural University of Hebei, Baoding 071001, China; 2.Department of Animal Science Hebei Normal University of Science & Technology, Changli 066600, China

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Abstract The polymorphism of mitochondrial DNA (mtDNA) D-loop of 83 individuals from nine Chinese indigenous sheep breeds and two imported sheep breeds was studied with five endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The average polymorphic degree (π value = 0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very low.

Issue Date: 05 September 2007

Cite this article:

LI Xianglong,ZHANG Zengli,LIU Zhengzhu, et al. mtDNA D-loop of Chinese main indigenous sheep breeds using PCR-RFLP[J]. Front. Agric. China, 2007, 1(3): 352-356.

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https://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0059-y
https://academic.hep.com.cn/fag/EN/Y2007/V1/I3/352

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