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Cytokines level changes in goats infected with |
| GU Youfang, WANG Bingyun, ZHANG Hongying, MAO Xinzhi, SHEN Yonglin |
| Animal Science College, Anhui Science and Technology University；Department of Animal Medicine, Foshan Science and Technology College；College of Veterinary Medicine, Nanjing Agricultural University |
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Abstract To study the changes of IL-2 and TNF-? in serum and the IL-2 secreted by peripheral blood lymphocytes of goats infected with Fasciola hepatica, thirty six clinically healthy white goats were randomly divided into groups coded as I, II, III, every 12 goats. Goats in group I and II were inoculated with a single oral dose of 200 and 500 encysted Fasciola hepatica metacercaria, respectively, while those in group III were untreated controls. Blood samples were collected from the jugular vein once a week for 11 weeks. Changes in the serum levels of IL-2 and TNF-? and the ability of peripheral blood lymphocytes to secrete IL-2 were detected. Serum IL-2 levels decreased in group I, but increased in group II. The levels of TNF-? and secreted IL-2 were elevated by varying degrees in both groups I and II. Lymphocytes displayed reactivity during the first week after infection with a significantly increased response to both specific and non-specific challenges. TNF-? may be involved in the hepatic injury process caused by Fasciola hepatica. The infective dose of Fasciola hepatica greatly influenced IL-2 levels in the host playing a vital role in the initiation of host defense.
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Issue Date: 05 June 2008
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| 1 |
Harbrecht B G Di Silvio M Demetris A J Simmons R L Billiar T R 1994 Tumor necrosis factor-alpharegulates in vivo nitric oxidesynthesis and induces liver injury during endotoxemiaHepatology2010551060.
doi:10.1002/hep.1840200439
|
| 2 |
Jin H Li X R Li T Yu K Z Lu J L 1998 High level expressionof recombinant bovine IL-2 gene in E. coli and detection of its biologicalactivity.Chinese Journal of Immunology14(4)313315 (in Chinese)
|
| 3 |
Leathers C W Foreyt W J Fetcher A Foreyt K M 1982 Clinical fasciolosis in domestic goats in MontanaJ Am Vet Med Assoc180(12)14511454
|
| 4 |
Martinez A Martinez-Cruz M S Martinez F J Gutierrez P N Hernandez S 1996 Detectionof antibodies to Fasciola hepatica excretory-secretory antigens in experimentally infected goats byenzyme immunosorbent assayVet parasitol62(3–4)247252.
doi:10.1016/0304‐4017(95)00876‐4
|
| 5 |
Mizia-Stec K Gasior Z Zahorska-Markiewicz B Janowska J Szulc A Jastrzebaka-Maj E Kobielusz-Gembala I 2003 Serum tumour necrosis factor-alpha, interleukin-2 and interleukin-10activation in stable angina and acute coronary syndromesCoron Artery Dis14(6)431438.
doi:10.1097/00019501‐200309000‐00003
|
| 6 |
Rickard L G 1995 Development and application of a dot-ELISA test for thedetection of serum antibodies to Fasciolahepatica antigens in llamasVetParasitol58(1–2)915.
doi:10.1016/0304‐4017(94)00709‐L
|
| 7 |
Wang P Y Jin J S Cai X P Duan Z Q Yuan W Z 1987 A field trial of ELISAin the diagnosis of Fasciola hepatica in sheepChinese Journal of VeterinaryScience and Technology7911 (in Chinese)
|
| 8 |
Yu K Z Li J T Zhen Y H Lu J L 1991 Detection of bovine interleukin-2 with MTT clorimetric assayChinese Journal of Animal and Poultry InfectiousDiseases32731 (in Chinese)
|
| 9 |
Zhang H Y Li H Z Niu Y X Wang F Y 1997 Tumor necrosis factor enhances macrophage mediated killing of Plasmodium yoeliiChinese Journal of Parasitology and Parasitic Diseases15(6)388391 (in Chinese)
|
| 10 |
Zhang W Moreau E Peiqne F Huang W Chauvin A 2005 Comparison of modulationof sheep, mouse and buffalo lymphocyte responses by Fasciola hepatica and Fasciola gigantica excretory-secretory productsParasitol Res95(5)333338.
doi:10.1007/s00436‐005‐1306‐x
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