Cloning, characterization and expression analysis
of two superoxide dismutase (SOD) genes in wheat ( L.)

doi:10.1007/s11703-008-0023-5

Front. Agric. China

2008, Vol. 2

Issue (2) : 141-149 https://doi.org/10.1007/s11703-008-0023-5

Cloning, characterization and expression analysis of two superoxide dismutase (SOD) genes in wheat ( L.)

ZHANG Haina, GUO Chengjin, LI Cundong, XIAO Kai

College of Agronomy, Agricultural University of Hebei;

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Abstract Superoxide dismutases (SODs) play an important role in catalyzing the conversion of O₂^- to H₂O₂, which can reduce the amount of harmful reactive oxygen specie (ROS) generated by the adverse environments, and alleviate the damage to plants. As one class of SODs, CuZnSODs have vital functions in preventing the ROS-generated cell damage and the death in aerobically growing organisms. In this study, two novel CuZnSOD genes in wheat, referred to TaSOD1.1 and TaSOD1.2 were identified, cloned and characterized. TaSOD1.1 and TaSOD1.2 were 780 bp and 1121 bp, respectively, predicting all to encode 201 amino acids. A 45-aa length of transit peptide at the N-terminal and a 79-aa conserved CuZn-SOD domain were respectively located in TaSOD1.1 and TaSOD1.2. Phylogenetic analysis indicated that the query SODs, most of CuZnSODs, could be classified into four subgroups. Compared with the control (CK), the abundance of TaSOD1.1 transcripts did not change under drought, salt, low and high temperature conditions, but the TaSOD1.2 transcripts were strongly induced by the above abiotic stresses, which was in accordance with the elevated SOD activities in leaves in the above stress treatments to some extent, suggesting its involvement in the plant’s acclimation and tolerance to the above abiotic stresses by possibly reducing the amount of the harmful ROS from enhancement of the SOD activity.

Issue Date: 05 June 2008

Cite this article:

ZHANG Haina,GUO Chengjin,LI Cundong, et al. Cloning, characterization and expression analysis of two superoxide dismutase (SOD) genes in wheat ( L.)[J]. Front. Agric. China, 2008, 2(2): 141-149.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0023-5
https://academic.hep.com.cn/fag/EN/Y2008/V2/I2/141

1	Alscher R G Donahue J L Cramer C L 1997 Reactive oxygen species and antioxidants:relationships in green cellsPhysiol Plant100224233. doi:10.1111/j.1399‐3054.1997.tb04778.x
2	Bowler C Van Camp W Van Montagu M Inzé D 1994 Superoxide dismutase in plantsCrit Rev Plant Sci13199218. doi:10.1080/713608062
3	Bowler C Van Montagu M Inzé D 1992 Superoxide dismutase and stress toleranceAnnu Rev Plant Physiol Mol Biol4383116. doi:10.1146/annurev.pp.43.060192.000503
4	Bueno P Varela J Gimenez G G Del Rio L A 1995 Peroxisomal copper, zinc superoxide dismutase: characterization ofthe isoenzyme from watermelon cotyledonsPlant Physiol10811511160. doi:10.1104/pp.108.3.1151
5	Cakamsk I Marschner H 1992 Magnesiumdeficiency and high light intensity enhance activities of superoxidedismutase, ascorbate peroxidata and glutathione ruductase in beanleavesPlant Physiol9812221227
6	Delseny M Cooke R Raynal M Grellet F 1997 The Arabidopsis thaliana cDNAsequencing projectsFEBS Lett405129132. doi:10.1016/S0014‐5793(97)00184‐1
7	Du J Zhu Z Li W C 2006 Over-expression of exotic superoxide dismutasegene MnSOD and increase in stressresistance in maizeJ Plant Physiol MolBiol32(1)5763 (in Chinese)
8	Du X M Yin W X Zhao Y X Zhang H 2001 The production and scavenging of reactive oxygen species in plantsChin J Biotech17121125 (in Chinese)
9	Frank V B Luit S Jean-Marie S Johan B Janya M Marc V M Dir K I 1999 Effects ofoverproduction of tobacco MnSOD in maize chloroplasts on foliar toleranceto cold and oxidative stressJ Exp Bot507178. doi:10.1093/jexbot/50.330.71
10	Hu G H Yu S X Fan S L Song M Z 2007 Cloning and expression of the chloroplast copper/zinc-superoxidedismutase gene in upland cotton (Gossypiumhirsutum L.).Journal of PlantPhysiology and Molecular Biology33197204
11	Jiang M Y Jing J H Wang S T 1991 Water stress and membrane-lipid peroxidationin plantsJ Northwest Agr Univ198894 (in Chinese)
12	Kliebenstein D J Monde R A Last R L 1998 Superoxide dismutase in Arabidopsis - an eclectic enzyme family withdisparate regulation and protein localizationPlant Physiol118637650. doi:10.1104/pp.118.2.637
13	Moradi F Ismail A M 2007 Responsesof photosynthesis, chlorophyll fluorescence and ROS-scavenging systemsto salt stress during seedling and reproductive stages in riceAnn Bot9911611173. doi:10.1093/aob/mcm052
14	Newman T de Bruijn F J Green P Keegstra K Kende H McIntosh L Ohlrogge J Raikhel N Somerville S Thomashow M 1994 Genes galore:a summary of methods for accessing results from large-scale partialsequence of anonymous Arabidopsis cDNA clonesPlant Physiol10612411255. doi:10.1104/pp.106.4.1241
15	Pastori G Foyer C H Mullineaux P 2000 Low temperature-induced changes in thedistribution of H₂O₂ and antioxidants between the bundle sheath and mesophyll cells ofmaize leavesJ Exp Bot51107113. doi:10.1093/jexbot/51.342.107
16	Prasad T K 1997 Role of catalase in inducing chilling tolerance in pre-emergentmaize seedlingsPlant Physiol11413691376
18	Rounsley S D Glodek A Sutton G Adams M D Somerville C R Venter J C Kerlavage A R 1996 The construction of Arabidopsis expressed sequence tagassembliesPlant Physiol11211771183. doi:10.1104/pp.112.3.1177
19	Runeckles V C Vaartnou M 1997 EPR evidencefor superoxide anion formation in leaves during exposure to low levelsof ozonePlant Cell Environ20306314. doi:10.1046/j.1365‐3040.1997.d01‐78.x
20	Scandalios J G 1997 Molecular genetics of superoxide dismutases in plantsScandalios J GOxidative Stress and the Molecular Biology of Antioxidant DefensesNYCold SpringHarbor Laboratory Press34527568
21	Shen X Y Xu S C Dai J Y 1995 Effects of drought on the activities ofSOD, CAT and acid phosphatase in maize leavesPlant Physiol Commun31183186
22	Simontacchi M Caro A Fraga C G Puntarulo S 1993 Oxidative stress affects α-tocopherol content insoybean embryonic axes upon inhibition and following germinationPlant Physiol103949953
23	Streller S Wingsle G 1994 Pinus sylvestris L. needles contain extracellularCuZn superoxide dismutase.Planta192195201. doi:10.1007/BF01089035
24	van Camp W Willekens H Bowler C van Montagu M Inze D Reupold-Popp P Sandermann H Langebartels C 1994 Elevated levelsof superoxide dismutase protect transgenic plants against ozone damageBio/Technol12165168. doi:10.1038/nbt0294‐165
25	Wolfe-Simon F Starovoytov V Reinfelder J R Schofield O Falkowski P G 2006 Localizationand role of manganese superoxide dismutase in a marine diatomPlant Physiol14217011709. doi:10.1104/pp.106.088963
26	Wu G Robertson A Wilen R Gusta L 1997 Plant electronic gene register PGR 97-024. Isolation and characterizationof two cDNAs (Accession Nos. U72212 (SOD3.1) and U73172 (SOD3.2)) encodingmitochondrial manganese superoxide dismutases in wheat.Plant Physiol113664. doi: 10.1104/pp.113.2.347
27	Wu G Wilen R Robertson A J Gusta L V 1999 Isolation, chromosomal localization, and differential expressionof mitochondrial manganese superoxide dismutase and chloroplasticcopper/zinc superoxide dismutase genes in wheatPlant Physiol120513520. doi:10.1104/pp.120.2.513
28	Wu G Wilen R Robertson A Gusta L 1996 The electronic plant gene register PGR 96-126. Molecular cloningof two wheat (Triticum aestivum) genes (Accession Nos. U69536 (SOD1.1) and U69632 (SOD1.2)) encodingchloroplast Cu/Zn superoxide dismutases.Plant Physiol1121736
29	Yruela I Pueyo J J Alonso P J Picorel R 1996 Photoinhibition of photosystem II from higher plants: effect of copperinhibitionJ Biol Chem2712740827415. doi:10.1074/jbc.271.44.27408
30	Zhu D Scandalios J G 1994 Differentialaccumulation of manganese-superoxide dismutase transcripts in maizein response to absicisic acid and high osmoticumPlant Physiol106173178

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