Establishment of transgenic acceptor and transformation
of  gene by particle gun
in maize inbred line 18-599 (white)

doi:10.1007/s11703-008-0019-1

Front. Agric. China

2008, Vol. 2

Issue (1) : 37-43 https://doi.org/10.1007/s11703-008-0019-1

Establishment of transgenic acceptor and transformation of gene by particle gun in maize inbred line 18-599 (white)

SUN Qingquan¹, DONG Shuting¹, ZHANG Chunqing¹, ZHANG Ying², MA Dengchao³

1.Shandong Agricultural University, State Key Laboratory of Crop Biology; 2.Maize Research Institute, Sichuan Agricultural University; 3.Jining Agricultural Science Institute;

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Abstract The efficient acceptors for maize transgenic engineering are currently insufficient in China. Seed production by male sterility is the best method for advancing the authenticity of maize hybrid. Maize inbred line 18-599 (white) is an antivirus high-quality maize inbred line in China, which has been used for lots of maize hybrid cultivars. The establishment of high efficiency transgenic acceptors is necessary for advancing the transgenic efficiency in maize transformation work. In this study, the efficient transgenic acceptors were optimized and established. 18-599 (white) was studied in state, types of culture mediums, times of callus regeneration and concentration of the screening reagent, Basta. The results showed that N6-4 medium was the best in 8 types of mediums for the immature embryo of 18-599 (white), 1.6 mm length was the feasible length of immature embryos for tissue culture in establishing the transgenic acceptor system, and it was within 5 times for suitable callus subculture. With the optimized transgenic acceptors, barnase gene was translated successfully into 18-599 (white) by a particle gun using bar as a marker gene. Basta was used as the screening reagent, its lethal concentration was 8 mg·L^-1 and its working concentration for screening was 6, 8 and 6 mg·L^-1 in 3 turns for callus regeneration, respectively. In this work, a transgenic plant with male sterility was obtained through molecule detection and observation in the field. The result has an important significance for the creation of new male sterility inbred lines in maize in the future.

Issue Date: 05 March 2008

Cite this article:

SUN Qingquan,DONG Shuting,ZHANG Chunqing, et al. Establishment of transgenic acceptor and transformation of gene by particle gun in maize inbred line 18-599 (white)[J]. Front. Agric. China, 2008, 2(1): 37-43.

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https://academic.hep.com.cn/fag/EN/10.1007/s11703-008-0019-1
https://academic.hep.com.cn/fag/EN/Y2008/V2/I1/37

1	Fu F L Li W C Rong T Z 2005 Effect of Ca²⁺ and uniconazole appended in N6 medium on immature embryos cultivatingin maizeActa Agron Sin31(5)634639(in Chinese)
2	Klein T M Wolf E D Wu R Sanford J C 1987 High velocity microprojectiles for delivery of nucleic acid intoliving cellsNature3277073
3	Li G S Zhang Q W Zhang J R 2001 Establishment of tuft bud system and regenerationof transgenic maize plants with resistance to weedicideSci Chin (Ser C)31(5)385391(in Chinese)
4	Li S G Liu Y L Zhu F Luo Y Y Kang L Y Tian B 1997 Genetically engineered male sterile tobacco plants andtheir sensitivity to temperatureActa BotSin39(3)231235(in Chinese)
5	Li S R Zhang J R Chen H M 1990 Study on abduction of embryonic callussubculture and regeneration of maize plantsJ Shandong Univ (Nat Sci Edn)25116124(in Chinese)
6	Ling D H Tao L Z Ma Z R Zhang S P Datta S K 1998 Engineered male steriletransgenic plants of rice (Oryza sativa L.) with ps1-barnase gene transformationby particle bombardment.Acta Genet Sin25(5)433442(in Chinese)
7	Lu G H Sun H T Zhang J L Hong M M 2000 Induction of male sterility by the integration of chimeric RTS-barnase gene into rice (Oryza sativa L.) genome. Acta Phytophysiol Sin26(2)171176
8	Mariani C 1990 Induction of male sterility in plants by a chimaeriericribonuiease geneNature347(25)737741
9	Mariani C Gossele V Beuckeleer N D 1992 A chimaeric ribonuclease inhibitor generestores fertility to male sterile plantsNature357384387
10	Murray M G Thompson W F 1980 Rapid isolationof high molecular weight plant DNANuclAcids Res843214326
11	Pan G T Xia Y L Liu Y Z Rong T Z 2003 Genetic variability analysis of embryo genic callus inductivity fromimmature embryo culture in maizeActa AgronSin29(3)386390(in Chinese)
12	Sun Q Q Zhang Y Rong T Z Dong S T Zuo Z P 2007 Transfer and detectionof barstar gene to maize inbred line 18-599 (white) by particle bombardmentAgricultural Sciences in China6(6)101105
13	Wan Y C Widholrn J M Lemaux P G 1995 Type I callus as a bombardrnent targentfor generating fertile transgenic maize (Zeamays L.). Planta196714
14	Wang G Y Du T B Zhang H 1995a Transfer of Bt-toxin protein gene intomaize and regeneration of transgenic maize plantsSci China (Ser B)25(1)7176(in Chinese)
15	Wang G Y Zhang H Xie Y J 1995b Transfer of Bt-toxin protein gene intomaize and resistance of transgenic plants to corn borerJ Agric Biol3(3)4953(in Chinese)
16	Zhan X Y Wu H M Cheung A Y 1996 Nuclear male sterility induced by pollen-specificexpression of a ribonucleaseSex Plant Reprod93543
17	Zhang L P Li W C Pan G T Zhu Z Rong T Z 2000 Efficient biolistic transformationof elite maize inbred linesJ Sichuan Univ375661(in Chinese)
18	Zhou X R Peng R W Fang R X Chen Z H Mang K Q 1997 Obtaining male sterilerape by specific expression of RNase geneActa Genet Sin24(6)531536(in Chinese)
19	Zou J T Zhan X Y Wu H M Cheung A Y 1994 Characterization of a rice pollen specific gene and its expressionAm J Bot81552561

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