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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 467-473     DOI: 10.1007/s11703-008-0076-5
Preliminary proteomics analysis of the total proteins of flower bud induction of apple trees
CAO Shangyin1, GUO Junying1, CHEN Yuling1, XUE Huabai1, ZHANG Qiuming2, ZHU Zhiyong3
1.Zhengzhou Fruit Research Institute, the Chinese Academy of Agricultural Sciences (CAAS); 2.College of Horticulture, Hunan Agricultural University; 3.College of Horticulture, Jiangxi Agricultural University;
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Abstract Apple is one of the most important fruit trees in the world. Nevertheless, mainly due to its long juvenile period, its breeding work constantly falls far behind other crops. So the aim of this study is to reveal the mechanism of apple flower bud differentiation, shorten the juvenile period and accelerate its breeding process. Proteomics technology (including two-dimensional gel electrophoresis (2-DE), biomass spectrometry and bioinformatics) was applied to work on the specific protein of flower bud and leaf bud after the brachyblasts of ‘Fuji’ stopped growth for 3–9 weeks. The results showed that the morphodifferentiation of flower bud did not begin until the seventh week after the brachyblast stopped growth. Furthermore, compared with the leaf bud, flower bud had significant changes in the expression of 283 protein spots in quality and quantity on 2-DE maps. Among the 283 protein spots, four protein spots (16.4, 30.2, 40.3 and 65.1 kD) were characteristic of the flower bud in the archae-stage (initial inflorescence appeared) at the beginning of flower-bud differentiation, three (39.3, 60.2 and 66.3 kD) in the post-stage (Lateral-flower appears) and one (77.1 kD) in the sepal stage on 2-DE maps. Analysis by peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry also identified and forecasted functionally by blasting different databases. In the four specific proteins, it was found that spots No. 256 (16.4 kD) and 298 (30.2 kD) were unknown proteins, spot Nos. 327 (40.3 kD) was identified as the synthesis enzyme protein and spot No. 367 (40.3 kD) was identified as a RNA-binding protein involved in transcription. When flower bud started to differentiate morphologically, we detected four specific proteins which were 16.4, 30.2, 40.3 and 65.1 kD. Three specific proteins 39.3, 60.2 and 66.3 kD were observed at side flower-appearing stage. When calyx began to emerge, there was one specific protein: 77.1 kD. The proteins 16.4 kD and 30.2 kD were two unknown specific proteins. The 40.3 kD protein was related to methionine synthetase while 65.1 kD protein, a RNA-binding protein, was related to transcription.
Issue Date: 05 December 2008
 Cite this article:   
GUO Junying,CAO Shangyin,CHEN Yuling, et al. Preliminary proteomics analysis of the total proteins of flower bud induction of apple trees[J]. Front. Agric. China, 2008, 2(4): 467-473.
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