Please wait a minute...
Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2008, Vol. 2 Issue (4) : 391-395     DOI: 10.1007/s11703-008-0070-y
Study on the culture of cut plants in wheat haploid embryo induction by a wheat × maize cross
GU Jian, LIU Kun, LI Shaoxiang, TIAN Yuxian, YANG Hexian, YANG Mujun
Food Crops Institute, Yunnan Academy of Agricultural Sciences;
 Download: PDF(78 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract The wheat × maize system is one of the most effective ways to produce haploids in wheat. Whether and how it could be successfully applied in practical breeding mostly depends upon the efficiency of haploid embryo production. To perfect the protocols of haploid embryo induction, the efficiency of haploid embryo production between in vitro culture of cut plant and intact plant growth for hybrid spikes with two F1 wheat hybrids and two maize varieties was compared. Effects of different cutting plant times and formulas of nutrient solutions for cut plant culture on haploid embryo formation were also studied. Results indicated that the embryo rate of in vitro culture was 3.29 times that of intact plant growth, with the figures of 31.6% vs 9.6%, respectively. The optimal time for cut plant culture was 24 h after pollination. Formulas of nutrient solutions significantly affected the efficiency of haploid embryo induction. With an embryo rate of 0–35.5%, adding calcium phosphate in the culture solution at 3 g·L-1 could raise the caryopsis and embryo rates. According to this study, the best medium for cut plant culture was: 100 mgL-1 2,4-D + 40 gL-1 sucrose + 10 mgL-1 silver nitrate + 8 mLL-1 sulfurous acid + 3 gL-1 calcium phosphate, with which a caryopsis rate of 95% and an embryo rate of about 30% could be obtained.
Issue Date: 05 December 2008
 Cite this article:   
LIU Kun,GU Jian,LI Shaoxiang, et al. Study on the culture of cut plants in wheat haploid embryo induction by a wheat × maize cross[J]. Front. Agric. China, 2008, 2(4): 391-395.
1 Aditya P, Gurdeep S S, Harinder K C (2006). Relative efficiency of anther cultureand chromosome elimination techniques for haploid induction in triticale× wheat and triticale × triticale hybrids. Euphytica, 50(3): 339–345
2 Almouslem A B, Jauhar P P, Peterson T S, Bommineni V R, Rao M B (1998). Haploiddurum wheat production via hybridization with maize. Crop Sci, 38(4): 1080–1087
3 Ballesteros J, Garcia-Llamas C, Ramirez M C, Martin A (2003). Low relative humidity increase haploid production in durum wheat× maize crosses. Plant Breeding, 122: 276–278.
4 Cai H, Ma C X, Si H Q, Qiao Y Q, Lu W Z (2005). Study on increasingfrequency of haploid embryo production through wheat × maize. Journal of Triticeae Crops, 25(6): 20–24 (in Chinese)
5 Campbell A W, Griffin W B, Burritt D J, Conner A J (2001). The importance of light intensity for pollen tube growth and embryosurvival in wheat × maize crosses. Ann Bot, 87: 517–522.
6 Chen X M, Chen X (1998). Statusof producing haploid and double haploid wheat through wheat ×maize. Journal of Triticeae Crops, 18(3): 1–4 (in Chinese)
7 Chen X M, Li X Y, Chen X, Meng F H, Cui S L (1998). Effect of differentcrossing techniques on haploid production in wheat × maize crosses. Acta Agronomica Sinica, 24(6): 743–746 (in Chinese)
8 Cherkaoui S, Lamsaouri O, Chlyah H (2000). Durum wheat × maize crossesfor haploid wheat production: influence of parental genotypes andvarious experimental factors. Plant Breeding, 119: 31–36.
9 Gu J, Liu K, Li S X, Tian Y X, Yang H X, Shan Q L, Yang M J (2006). Preliminarystudy on using wheat × maize to induce wheat haploid plant innatural condition of Kunming. Journal ofTriticeae Crops, 26(4): 23–26 (in Chinese)
10 Gu J, Tian Y X, Li S X, Liu K, Yang H X, Yang M J (2005). Effects of wheat floret position and development statuson haploid embryo rate of wheat × maize crosses. Journal of Triticeae Crops, 25(1): 30–32 (in Chinese)
11 Inagaki M N (1997). Use of pollen storage and detached tiller culture inwheat polyploidy production through wide cross. Cereal Res Comm, 25: 7–13
12 Inagaki M N, Tahir M (1992). Productionof haploid wheat through intergeneric crosses. Hereditas, 116: 117–120.
13 Lai C K, Wang C S, Yan L (2007). Study on haploid embryo productionof wheat by distant hybridization between winter wheat and maize. Journal of Triticeae Crops, 27(2): 193–196 (in Chinese)
14 Laurie D A (1989). Factors affecting fertilization frequency in crossesof Triticum aestivum cv. ‘Highbury'× Zea mays cv. ‘Seneca60′. Plant Breeding, 103(2): 133–140.
15 Laurie D A, Bennett M D (1986). Wheat× maize hybridization. Canadian Journalof Genetics and Cytology, 28: 313–316
16 Laurie D A, Reymondie S (1991). Highfrequencies of fertilization and haploid seedling production in crossesbetween commercial hexploid wheat varieties and maize. Plant Breeding, 106: 182–189.
17 Li D W, Qio J W, Ouyang P, Yao Q X (1996). High frequencies of fertilization and embryo formation in hexaploidwheat × Tripsacum dactyloides crosses. Theor Appl Genet, 92: 1103–1107
18 Lü G F, Fan J P, Zhang B Q, Gao D R, Lu C B, Zhang Y, Wu H Y, Cheng S H (2002). Study on the frequencies of haploidwheat embryo production in wheat × maize crosses. Jiangsu Agricultural Science, 5: 9–11 (in Chinese)
19 Riera-Lizarazu O, Mujeeb-Kazi A, William M D H M (1992). Maize (Zeamays L.) mediated polyploid production in some Triticeae using a detached tiller method. J Gene Breed, 46: 335–346
20 Suenaga K, Morshedi A R, Darvey N L (1997). Haploid production of Australianwheat (Triticum aestivum L.) cultivarsthrough wheat × maize (Zea mays L.) crosses. Aust J Agric Res, 48: 1207–1211.
21 Sun J S, Liu H, Lu T G, Wang X A, Ren Z, Wang J L (1992). The production of haploid wheat plants via wheat × maize hybridization.Acta Botanica Sinica, 34(11): 817–821 (in Chinese)
22 Vinesh Verma, Bains N S, Mangat G S, Nanda G S, Gosal S S, Singh K (1999). Maize genotypes show striking differences for inductionand regeneration of haploid wheat embryos in wheat × maize system. Crop Sci, 39: 1722–1727
23 Wang G J (1998). Study on the haploid efficiency produced by wheat ×maize crosses. Journal of Triticeae Crops, 18(6): 12–14 (in Chinese)
Full text