|
|
Suitable condition of enzymatic reactivity for
determining the digestibility of feedstuffs phosphorus by dialysis tube method |
ZUO Jianjun, DONG Zemin, YAN Xiling, YE Hui, CAO Qingyun, FENG Dingyuan |
College of Animal Science, South China Agricultural University |
|
|
Abstract In order to develop a new method for determining the phosphorus (P) digestibility in vitro in feedstuffs by dialysis tube, a L32(49) orthogonal experiment with eight factors (4 levels for each factor) and a single factor experiment on the enzymatic reactivity were carried out. The sequence of significance of the eight factors on sample-P dialyzability was as follows: trypsin digestion for 6 h, dialyzing solution at 100 mL, pH of pepsin solution at 2.5, pepsin concentration at 2000 UmL-1 pepsin digestion for 100 min, at temperature of 35°C, trypsin concentration at 1625 UmL-1, and pH of trypsin solution at 6.5, respectively. And in vitro dialyzabilities of P in soybean meal, barley, sorghum, peanut meal, and rapeseed meal were (36.91 ± 0.58)%, (27.28 ± 0.94)%, (26.95 ± 0.58)%, (30.51 ± 0.83)%, and (20.82 ± 1.09)%, respectively.
|
Issue Date: 05 December 2008
|
|
1 |
Fan M Z, Archbold T, Sauer W C, Lackeyram D, Rideout T, Gao Y X, de Lange C F M, Hacker R R (2001). Novelmethodology allows simultaneous measurement of true phosphorus digestibilityand the gastrointestinal endogenous phosphorus outputs in studieswith pigs. J Nutr, 131: 2388–2396
|
2 |
Fang R J (2003). True digestibility of phosphorus and predicted modelof truly digestible phosphorus in plant feedstuffs for growing pigs. Dissertation for the Doctoral Degree. Ya'an: SichuanAgricultural University, 60–65 (in Chinese)
|
3 |
Gauthier S F, Vachon C, Jones J D, Savoie L (1982). Assessment of protein digestibility by invitro enzymatic hydrolysis with simultaneous dialysis. J Nutr, 112: 1718–1725
|
4 |
Huang R L, Li T J, Tan Z L, Xing T X (1999). Study on the suitable condition of enzymatic reactivity for determiningthe disappearance of sample crude protein in vitro by dialysis tube method. Acta Zoonutrimenta Sinica, 11(4): 51–58 (in Chinese)
|
5 |
Huo Q G (2002). Animal Nutrition and Source of Phosphorus. Beijing: China Agricultural Science and Technology Press, 114–115 (in Chinese)
|
6 |
Ji C, Rong Y, Schenermann S E, Xu W G (1994). Measurement of availability of amino acids in poultry feedstuff bypepsin in vitro. Journal of Agricultural Biotechnology, 2(2): 103–109 (in Chinese)
|
7 |
Liu J Z, Ledoux D R, Veum T L (1997). In vitro procedure for predicting the enzymatic dephosphorylation of phytatein corn-soybean meal diets for growing swine. J Agric Food Chem, 45(7): 2612–2617. doi:10.1021/jf970402z
|
8 |
Mauron J, Mottu F, Bujard E, Ejli R H (1955). The availability of lysine, methionine and tryptophan in condensedmilk and milk powder. In vitro digestionstudies. Arch Biochem Biophys, 59: 433–451. doi:10.1016/0003-9861(55)90510-5
|
9 |
Savioe L, Gauthier S F (1986). Dialysiscell for the in vitro measurementof protein digestibility. J Food Sci, 51: 494–498. doi:10.1111/j.1365-2621.1986.tb11164.x
|
10 |
Steinhart H, Kirchgessner H (1973). In vitro verdaungsapparatur zur enzymatischenhydrolyse von proteinen. Arch Tierernahr(Abstract), 23: 449–461
|
11 |
Xi P B, Li D F, Zheng C T (2003). Enzymatic conditions of an in vitro method using dialysis tube to studydigestion of protein in rapeseed meal. Journal of China Agricultural University, 8(5): 88–92 (in Chinese)
|
12 |
Zhang L Y (2003). Feeding Stuffs-analysis and Determination Technology. Beijing: China Agricultural University Press, 134–136 (in Chinese)
|
13 |
Zhang L Y (2003). Feeding Stuffs-analysis and Determination Technology. Beijing: China Agricultural University Press, 136–138 (in Chinese)
|
14 |
Zyla K, Gogol D, Koreleski J, Swiatkiewicz S, Ledoux D R (1999). Simultaneousapplication of phytase and xylanase to broiler feeds based on wheat: in vitro measurements of phosphorus and pentoserelease from wheats and wheat-based feeds. J Sci Food Agric, 79(13): 1832–1840. doi:10.1002/(SICI)1097-0010(199910)79:13<1832::AID-JSFA441>3.0.CO;2-Q
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|