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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 383-387     DOI: 10.1007/s11703-009-0071-5
Research articles |
SRAP marker reveals genetic diversity in tartary buckwheat in China
Yanqin LI1,Xiaoling FAN1,Tonglin SHI1,Quanbin ZHANG1,Zongwen ZHANG2,
1.Key Laboratory for Chemical Biology and Molecular Engineering of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, China; 2.The Chinese Academy of Agricultural Sciences, Beijing 100081, China;
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Abstract  Sequence-related amplified polymorphism (SRAP) marker was employed to analyze genetic diversity of 10 accessions of tartary buckwheat selected from a wide geographical area in China. Of the total 30 primer combinations investigated, 26 could amplify clearly and consistently. They produced a total of 285 fragments, of which 235 (82.5%) were polymorphic bands. Among the 26 primer combinations, five could discriminate all the genotypes used in this study. Based on the molecular data, the genetic similarity coefficients varied from 0.61 to 0.78 and calculated using the NTSYSpc published by Nei and Li (1979). The cluster analysis revealed that the 10 accessions were better to be grouped into two major clusters at a similarity level of 0.69. Moreover, the accessions collected from the same province turned out to be grouped in the same cluster, which indicated some geographical relationships. It also proved that the SRAP marker system was useful in identification and genetic diversity analysis of tartary buckwheat.
Keywords genetic diversity      NTSYS-pc      SRAP marker      tartary buckwheat      
Issue Date: 05 December 2009
URL:     OR
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