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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 413-418     DOI: 10.1007/s11703-009-0077-z
Research articles |
Cloning and expression analysis of a ribosomal protein S7 in wheat under the stress of Puccinia triticina
Aihua YAN,Yunwei ZHANG,Lifeng ZHANG,Dongmei WANG,
College of Life Science, Agricultural University of Hebei, Baoding 071001, China;
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Abstract  A gene was isolated from the suppression subtractive hybridization (SSH) cDNA library of wheat by reverse northern blotting. Full length cDNA was obtained by RACE technique, and the result showed that all sequences consisted of 471 bp and encoded 157 amino acid. Subsequently, the gene expression was enhanced according to quantity real-time PCR (qRT-PCR) in response to leaf rust stress as the time over. All the results above suggested that the gene might play important role in wheat hypersensitive response (HR) to leaf rust.
Keywords wheat      ribosomal protein      cloning      expression      
Issue Date: 05 December 2009
URL:     OR
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