Strawberry FaEtr2 gene RNAi expression vector construction and genetic transformation

doi:10.1007/s11703-009-0057-3

Front. Agric. China

2009, Vol. 3

Issue (4) : 419-424 https://doi.org/10.1007/s11703-009-0057-3

Research articles

Strawberry FaEtr2 gene RNAi expression vector construction and genetic transformation

Chunli SONG¹,Junlian MA²,Xia TANG²,Zide ZHANG²,Zhixia HOU³,

1.College of Chinese Veterinary, Agricultural University of Hebei, Dingzhou 073000, China; 2.College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 3.The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China;

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Abstract The short hairpin RNA (shRNA) expression vector of the FaEtr2 gene was constructed by inserting the sense fragment into the constructed antisense vector of FaEtr2 (pBI121-Anti-Etr2) in sense orientation. The constructed RNA interference (RNAi) expression vector was transformed into Agrobacterium fumefeciens LBA4404 and used to infect strawberry leaves. Using in vitro plantlet leaves as explants, the transformation conditions of All-Star strawberry were studied systemically. The results showed that infecting the leaves with A. fumefeciens resuspension liquid of OD₆₀₀ = 1.0 by pre-culturing for 3 d, co-culturing for 3 d, infecting for 10 min, and adding acetosyringone (AS) 50―100 µmol·L^−1 was suitable for genetic transformation of All-Star strawberry. Seven lines of transgenic plants were preliminarily identified by PCR and β-glucurondiase (GUS) histochemical staining.

Keywords strawberry FaEtr2 RNA interference (RNAi) genetic transformation

Issue Date: 05 December 2009

Cite this article:

Xia TANG,Zhixia HOU,Chunli SONG, et al. Strawberry FaEtr2 gene RNAi expression vector construction and genetic transformation[J]. Front. Agric. China, 2009, 3(4): 419-424.

URL:

https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0057-3
https://academic.hep.com.cn/fag/EN/Y2009/V3/I4/419

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