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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 419-424     DOI: 10.1007/s11703-009-0057-3
Research articles |
Strawberry FaEtr2 gene RNAi expression vector construction and genetic transformation
Chunli SONG1,Junlian MA2,Xia TANG2,Zide ZHANG2,Zhixia HOU3,
1.College of Chinese Veterinary, Agricultural University of Hebei, Dingzhou 073000, China; 2.College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China; 3.The Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China;
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Abstract  The short hairpin RNA (shRNA) expression vector of the FaEtr2 gene was constructed by inserting the sense fragment into the constructed antisense vector of FaEtr2 (pBI121-Anti-Etr2) in sense orientation. The constructed RNA interference (RNAi) expression vector was transformed into Agrobacterium fumefeciens LBA4404 and used to infect strawberry leaves. Using in vitro plantlet leaves as explants, the transformation conditions of All-Star strawberry were studied systemically. The results showed that infecting the leaves with A. fumefeciens resuspension liquid of OD600 = 1.0 by pre-culturing for 3 d, co-culturing for 3 d, infecting for 10 min, and adding acetosyringone (AS) 50―100 µmol·L−1 was suitable for genetic transformation of All-Star strawberry. Seven lines of transgenic plants were preliminarily identified by PCR and β-glucurondiase (GUS) histochemical staining.
Keywords strawberry      FaEtr2      RNA interference (RNAi)      genetic transformation      
Issue Date: 05 December 2009
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