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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2009, Vol. 3 Issue (4) : 431-434     DOI: 10.1007/s11703-009-0072-4
Research articles |
Evaluation of two-dimensional gel electrophoresis-based technology on plant calmodulin isoforms
Chunyan HOU,Aihua YAN,Jie ZHANG,Hui ZHOU,Shuchao LV,Dongmei WANG,
College of Life Science, Agricultural University of Hebei, Baoding 071001, China;
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Abstract  The two-dimensional gel electrophoresis-based technique is commonly used in protein analysis and identification. Though this technique is powerful, mature and sensitive there still remain some questions regarding its ability to characterize different types of proteomes. Different calmodulin isoforms play different roles in response to varying external stimulant signals during plant development, but their advanced structures are too small to be distinguished and purified at protein level. In this paper, a two-dimensional gel electrophoresis-based technology was investigated to identify different calmodulin isoforms, which will lay a foundation for subsequent researches.
Keywords 2D gel electrophoresis      plant calmodulin      isoform      
Issue Date: 05 December 2009
URL:  
http://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0072-4     OR     http://academic.hep.com.cn/fag/EN/Y2009/V3/I4/431
Duval F D, Renard M, Jaquinod M, Biou V, Montrichard F, Macherel D(2002). Differentialexpression and functional analysis of three calmodulin isoforms ingerminating pea (Pisum sativum L.)seeds. Plant Journal, 32: 481–493

doi: 10.1046/j.1365-313X.2002.01409.x
Eriko I, Ryuji S, Fumiya K(2005). Multiple forms ofcalmodulin genes in carrot treated with fungal mycelial walls. Biol Pharm Bull, 28(6): 1109–1112

doi: 10.1248/bpb.28.1109
Eriko I, Tetsuya A(2004). Cloning and expression of calmodulin genes regulatingphytoalexin production in carrot cells. Biol Pharm Bull, 27(8): 1308–1311

doi: 10.1248/bpb.27.1308
Heo W D, Lee S H, Kim M C, Kim J C, Chung W S, Chun H J, Lee K J, Park C Y, Park H C, Choi J Y, Cho M J(1999). Involvement of specific calmodulinisoforms in salicylic acid-independent activation of plant diseaseresistance responses. Proc Natl Acad SciUSA, 96: 766–771

doi: 10.1073/pnas.96.2.766
Hetherington A M, Brownlee C(2004). The generation of Ca2+ signalin plants. Annu Rev Plant Biol, 55: 401–427

doi: 10.1146/annurev.arplant.55.031903.141624
Lee S H, Kim J C, Lee M S, Heo W D, Seo H Y, Yoon H W, Hong J C, Lee S Y, Bahk J D, Hwang I, Cho M J(1995). Identification of a novel divergentcalmodulin isoform from soybean which has differential ability toactivate calmodulin-dependent enzymes. Journal of Biological Chemistry, 270: 21806–21812

doi: 10.1074/jbc.270.37.21806
Reona T, Eri K, Shigemi S, Ichiro M, Kazuyuki K, Yuko O(2007). Pathogen-induced calmodulin isoformsin basal resistance against bacterial and fungal pathogens in tobacco. Plant Cell Physiol, 48(3): 414–423

doi: 10.1093/pcp/pcm011
Sun D Y, Guo Y L, Ma L G, Cui S J(2001). Cell Signal Transduction. Beijing: Science Press
Takezawa D, Liu Z H, An G, Poovaiah B W(1995). Calmodulin gene family in potato: developmental andtouch-induced expression of mRNA encoding a novel isoform. Plant Molecular Biology, 27: 693–703

doi: 10.1007/BF00020223
Yang T, Segal G, Abbo S, Feldman M, Fromm H(1996). Characterizationof the calmodulin gene family in wheat: structure, chromosomal location,and evolutionary aspects. Mol Gen Genet, 252: 684–694

doi: 10.1007/BF02173974
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