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Evaluation of two-dimensional gel electrophoresis-based
technology on plant calmodulin isoforms |
| Chunyan HOU,Aihua YAN,Jie ZHANG,Hui ZHOU,Shuchao LV,Dongmei WANG, |
| College of Life Science,
Agricultural University of Hebei, Baoding 071001, China; |
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Abstract The two-dimensional gel electrophoresis-based technique is commonly used in protein analysis and identification. Though this technique is powerful, mature and sensitive there still remain some questions regarding its ability to characterize different types of proteomes. Different calmodulin isoforms play different roles in response to varying external stimulant signals during plant development, but their advanced structures are too small to be distinguished and purified at protein level. In this paper, a two-dimensional gel electrophoresis-based technology was investigated to identify different calmodulin isoforms, which will lay a foundation for subsequent researches.
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| Keywords
2D gel electrophoresis
plant calmodulin
isoform
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Issue Date: 05 December 2009
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