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Frontiers of Agriculture in China

ISSN 1673-7334

ISSN 1673-744X(Online)

CN 11-5729/S

Front. Agric. China    2010, Vol. 4 Issue (1) : 74-78    https://doi.org/10.1007/s11703-009-0091-1
Research articles
The genomic sequence of AFS-1―an alpha -farnesene synthase from the apple cultivar ‘Royal Gala’
Lesley BEUNING,Sol GREEN,Yar-Khing YAUK,
The New Zealand Institute for Plant & Food Research Limited, Mt Albert, Auckland 1025, New Zealand;
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Abstract The genomic sequence encoding alpha- farnesene synthase-1 (AFS-1) was amplified from genomic DNA isolated from ‘Royal Gala’ apple (Malus×domestica Borkh.). The genomic sequence consists of six introns and seven exons, which is consistent with Class III terpene synthases. Four variants of the genomic sequence were amplified. The four variants are based on the presence or absence of a repeat of two sequences, one found in intron 4 (CAGTTATTTAATT) and the other in intron 5 (TA). Although there were small nucleotide differences among the three apple cultivars ‘Royal Gala’, ‘Idared’, and ‘Ralls’, these resulted in only two amino acid changes in the protein sequence, which are unlikely to explain the resistance or susceptibility of an apple cultivar to superficial scald. Given that AFS-1 transcript levels are high in all cultivars, it appears that it is either the reactions downstream of alpha-farnesene production that control the accumulation of oxidation products related to superficial scald or that the variation in the level of its substrate, farnesyl diphosphate, may cause differences in the amount of alpha-farnesene produced.
Keywords α-farnesene synthase      ‘Royal Gala’      genomic sequence      superficial scald      
Issue Date: 05 March 2010
 Cite this article:   
Lesley BEUNING,Sol GREEN,Yar-Khing YAUK. The genomic sequence of AFS-1―an alpha -farnesene synthase from the apple cultivar ‘Royal Gala’[J]. Front. Agric. China, 2010, 4(1): 74-78.
 URL:  
https://academic.hep.com.cn/fag/EN/10.1007/s11703-009-0091-1
https://academic.hep.com.cn/fag/EN/Y2010/V4/I1/74
Bohlmann J, Meyer-Gauen G, Croteau R (1998). Plant terpenoid synthases: Molecularbiology and phylogenetic analysis. Proceedingsof the National Academy of Sciences of the United States of America, 95(8): 4126–4133

doi: 10.1073/pnas.95.8.4126
Bohlmann J, Phillips M, Ramachandiran V, Katoh S, Croteau R (1999). cDNA cloning,characterization, and functional expression of four new monoterpenesynthase members of the Tpsd gene family from grand fir (Abies grandis). Archives of Biochemistry and Biophysics, 368(2): 232–243

doi: 10.1006/abbi.1999.1332
Davis E M, Croteau R (2000). Cyclizationenzymes in the biosynthesis of monoterpenes, sesquiterpenes, and diterpenes. Topics in Current Chemistry, 209: 53–95

doi: 10.1007/3-540-48146-X_2
Fernandez-Trujillo J P, Nock J F, Kupferman E M, Brown S K, Watkins C B (2003). Peroxidaseactivity and superficial scald development in apple fruit. J Agric Food Chem, 51(24): 7182–7186

doi: 10.1021/jf034079d
Green S, Friel E N, Matich A, Beuning L L, Cooney J M, Rowan D D, MacRae E (2007). Unusual features of a recombinant apple alpha-farnesenesynthase. Phytochemistry, 68(2): 176–188

doi: 10.1016/j.phytochem.2006.10.017
Green S, Squire C J, Nieuwenhuizen N J, Baker E N, Laing W (2009). Definingthe potassium binding region in an apple terpene synthase. J Biol Chem, 284(13): 8661–8669

doi: 10.1074/jbc.M807140200
Huang J, Cardoza Y J, Schmelz E A, Raina R, Engelberth J, Tumlinson J H (2003). Differential volatile emissions andsalicylic acid levels from tobacco plants in response to differentstrains of Pseudomonas syringae. Planta, 217(5): 767–775

doi: 10.1007/s00425-003-1039-y
López-Gómez R, Gómez-Lim M A (1992). A method for extracting intact RNA from fruits rich in polysaccharidesusing ripe mango mesocarp. Hortscience, 27: 440–442
Martin D M, Faldt J, Bohlmann J (2004). Functional characterization of nineNorway Spruce TPS genes and evolution of gymnosperm terpene synthasesof the TPS-d subfamily. Plant Physiology, 135(4): 1908–1927

doi: 10.1104/pp.104.042028
Newcomb R D, Crowhurst R N, Gleave A P, Rikkerink E H A, Allan A C, Beuning L L, Bowen J H, Gera E, Jamieson K R, Janssen B J, Laing W A, McArtney S, Nain B, Ross G S, Snowden K C, Souleyre E J F, Walton E F, Yauk Y-K (2006). Analyses of expressed sequence tags from apple. Plant Physiology, 141(1): 147–166

doi: 10.1104/pp.105.076208
Pare P W, Tumlinson J H (1998). Cottonvolatiles synthesized and released distal to the site of insect damage. Phytochemistry, 47(4): 521–526

doi: 10.1016/S0031-9422(97)00442-1
Pare P W, Tumlinson J H (1999). Plantvolatiles as a defense against insect herbivores. Plant Physiology, 121(2): 325–332

doi: 10.1104/pp.121.2.325
Pechous S W, Waltkins B D, Whitaker B D (2005). Expression of alpha-farnesene synthasegene AFS1 in relation to levels of alpha-farnesene and conjugatedtrienols in peel tissue of scald-susceptible ‘Law Rome’and scald-resistant ‘Idared’ apple fruit. Postharvest Biology and Technology, 35: 125–132

doi: 10.1016/j.postharvbio.2004.08.005
Pesis E, Ibanez A M, Phu M L, Mitcham E J, Ebeler S E, Dandekar A M (2009). Superficial scald and bitter pit developmentin cold-stored transgenic apples suppressed for ethylene biosynthesis. J Agric Food Chem, 57(7): 2786–2792

doi: 10.1021/jf802564z
Rowan D D, Hunt M B, Fielder S, Norris J, Sherburn M S (2001). Conjugatedtriene oxidation products of alpha-farnesene induce symptoms of superficialscald on stored apples. J Agric Food Chem, 49(6): 2780–2787

doi: 10.1021/jf0015221
Rueger B, Thalhammer J, Obermaier I, Gruenewald-Janho S (1996). Experimental procedure for the detection of rare humanmRNA with the DIG system. Biochemica, 3: 35–38
Sharkey T D, Yeh S, Wiberley A E, Falbel T G, Gong D, Fernandez D E (2005). Evolution of the isoprene biosyntheticpathway in kudzu. Plant Physiology, 137(2): 700–712

doi: 10.1104/pp.104.054445
Trapp S C, Croteau R B (2001). Genomicorganization of plant terpene synthases and molecular evolutionaryimplications. Genetics, 158(2): 811–832
Tsantili E, Gapper N E, Arquiza J M, Whitaker B D, Watkins C B (2007). Ethyleneand alpha-farnesene metabolism in green and red skin of three applecultivars in response to 1-methylcyclopropene (1-MCP) treatment. J Agric Food Chem, 55(13): 5267–5276

doi: 10.1021/jf063775l
Vuorinen T, Nerg A M, Ibrahim M A, Reddy G V, Holopainen J K (2004). Emissionof Plutella xylostella-inducedcompounds from cabbages grown at elevated CO2 and orientation behavior of the natural enemies. Plant Physiology, 135(4): 1984–1992

doi: 10.1104/pp.104.047084
Wang Z Y, Dilley D R (1999). Controlof superficial scald of apples by low-oxygen atmospheres. Hortscience, 34(7): 1145–1151
Whitaker B D (1998). Phenolic fatty-acid esters from the peel of ‘Gala’apples and their possible role in resistance to superficial scald. Postharvest Biology and Technology, 13(1): 1–10

doi: 10.1016/S0925-5214(97)00070-7
Whitaker B D (2004). Oxidative stress and superficial scald of apple fruit. Hortscience, 39(5): 933–937
Yuan K, Liu Q, Li B, Zhang L (2008). Genomic structure and sequence polymorphism of E,E-alpha-farnesenesynthase gene in apples (Malus domestica Borkh.). Frontiers of Agriculture inChina, 2(2): 190–193

doi: 10.1007/s11703-008-0041-3
[1] Kejun YUAN, Lixiang Huang, Chengxiang AI, Hairong WEI, Qingzhong LIU. Genomic organization and sequence polymorphism of a farnesyl diphosphate synthase gene in apples (Malus domestica Borkh.)[J]. Front Agric Chin, 2011, 5(2): 209-214.
[2] YUAN Kejun, LIU Qingzhong, LI Bo, ZHANG Lisi. Genomic structure and sequence polymorphism of E,E-alpha-farnesene synthase gene in apples ( Borkh.)[J]. Front. Agric. China, 2008, 2(2): 190-193.
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