%A FAN Jinghui, ZUO Yuzhu, LI Tanqing, ZHANG Xiaobo, ZHAO Yuelan
%T Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain
%0 Journal Article
%D 2007
%J Front. Agric. China
%J Frontiers of Agriculture in China
%@ 1673-7334
%R 10.1007/s11703-007-0060-5
%P 357-360
%V 1
%N 3
%U {https://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0060-5
%8 2007-09-05
%X The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.