%A FAN Jinghui, ZUO Yuzhu, LI Tanqing, ZHANG Xiaobo, ZHAO Yuelan %T Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain %0 Journal Article %D 2007 %J Front. Agric. China %J Frontiers of Agriculture in China %@ 1673-7334 %R 10.1007/s11703-007-0060-5 %P 357-360 %V 1 %N 3 %U {https://academic.hep.com.cn/fag/EN/10.1007/s11703-007-0060-5 %8 2007-09-05 %X The nucleocapsid protein gene of transmissible gastroenteritis virus, 1 149 bp in length, was amplified by RT-PCR from isolated strain HB06 and cloned into pMD18-T. Sequence comparison with other transmissible gastroenteritis virus (TGEV) strains selected from the Gene Bank revealed that the homology of N gene complete sequence shares more than 97% in nucleotide. N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. After being induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the recombinant nucleocapsid protein was expressed. The result of SDS-PAGE and Western-blot showed that the recombinant nucleocapsid protein was 47 kDa and had strong positive reactions with TGEV-specific antibody.