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Preparation of D-lysine by chemical reaction
and microbial asymmetric transformation |
LIU Yi1, JIAO Qingcai2, YIN Xiaoxing3 |
1.Department of Pharmacy, Xuzhou Medical College; State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University; 2.State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University; 3.Department of Pharmacy, Xuzhou Medical College |
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Abstract The DL-lysine crystals from the racemization of L-lysine was treated as substrate with Hafnia alvei AS1.1009 intact cells as biocatalysts to produce crystalline D-lysine with a yield of 56.6% from the reaction mixture after simple purification. In the presence of 0.10 molar equivalent of salicylaldehyde, L-lysine racemization can be completed within 4 h in 1.0 mol/L of NaOH at 100°C. The activation energy of the processes was 62187.86 J/mol. The characteristics of Hafnia alvei AS1.1009 decarboxylase were studied. Under the conditions of pH 8.0, temperature 37°C, cell concentration 10 g/L, tween-80 0.5 g/L, substrate concentration 30 g/L, and the specific activity of up to 3840 U, L-lysine can be completely degraded by the decarboxylase for 12 h under the optimal conditions.
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Issue Date: 05 March 2008
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1 |
Bajusz S Janaky T Csernus J Bokser L Fekete M Srkalovic G Redding T W Schally A V Highly potent metallopetide analogs of luteinizing hormone-releasinghormoneProc Natl Acad Sci USA 1989 86(16)63136317
|
2 |
Shen W C Du X Feener E P Ryser H J P The intracellularrelease of methotrexate from a synthetic drug carrier system targetedto Fc receptor-bearing cellsJ ControlledRelease 1989 108996
|
3 |
Liao X H Studyon the production of D-lysine monohydrochloride from L-lysine monohydrochlorideAmino Acids and Biotic Resources 1999 21(3)13013l(in Chinese)
|
4 |
Chemical IndustryPress organizing to compile. Encyclopedia of Chinese ChemicalProducts (Third Edition)BeijingChemical Industry Press 2005 12451260(in Chinese)
|
5 |
Sun Z Q 2005lysine market review and 2006 outlookFeedReview2006(3)3436(in Chinese)
|
6 |
Yamada S Hongo C Yoshioka R Chibata I Method for theracemization of optically active amino acidJ Org Chem 1983 48(6)843846
|
7 |
Liu Y Guo L Y Wu X Y Jiao C Q Microwave-assistedrapid racemization of L-amino acidsChemistry 2005 68(12)935938(in Chinese)
|
8 |
Ebbers E J Ariaans G J A Houbiers J P M Bruggink Alle. Zwanenburg B Controlled racemization ofoptically active organic compounds: prospects for asymmetric transformationTetrahedron 1997 53(28)94179476
|
9 |
Takahashi E Furui M Shibatani T Scale-up of D-lysine production from L-lysine by successivechemical racemization and microbial asymmetric degradationBiotechnology Letters 1997 19(3)245249
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