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Application of high-speed counter-current chromatography
coupled with high performance liquid chromatography for the separation
and purification of Quercetin-3- O -sambubioside from the leaves of Nelumbo
nucifera |
| Shengguo DENG,Zeyuan DENG,Yawei FAN,Jing LI,Rong LIU,Dongmei XIONG, |
| State Key Lab of Food
Science and Technology, Institute for Advanced Study, Nanchang University,
Nanchang 330047, China; |
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Abstract Quercetin-3-O-sambubioside [Quercetin-3-O-β-D-xylopyranosyl (1→2)-β-D-glucopyranoside] was separated and purified by semi-preparative high-speed counter-current chromatography (HSCCC) with a two-phase-solvent system composed of ethyl acetate-n-butanol–water (4∶1∶5, v/v) from the leaves of Nelumbo nucifera (Lotus). A total of 5.0mg of the targeted compound with a purity of 98.6% as determined by high performance liquid chromatography (HPLC) was obtained from 100m g of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation. Quercetin-3-O-sambubioside was a novel flavonoid glycoside from the leaves of Nelumbo nucifera, and its chemical structure was identified by means of ESI-MS, 1D NMR and 2D NMR.
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Issue Date: 05 December 2009
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