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Purification of ice structuring protein complexes
from winter wheat using Triton X-114 phase partitioning |
Huaneng XU,Haiying CHEN,Weining HUANG, |
State Key Laboratory
of Food Science and Technology, School of Food Science and Technology,
Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; |
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Abstract Ice structuring proteins (ISPs) isolated from the cold-acclimated plants have a great potential in improving the quality of frozen foods. The purification of ISP complexes from winter wheat was achieved using an aqueous two-phase system of Triton X-114. The highly reactive phenols were removed, and the ISP complexes remained in the aqueous phase after phase separation. The ISP complexes treated by this procedure retained higher thermal hysteresis activity than those treated by ammonium sulfate method. The phase separation technique provides a simple and mild way for removing phenols from ISP complexes.
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Issue Date: 05 December 2009
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