Biological pretreatment of corn stover by solid state fermentation of <italic>Phanerochaete chrysosporium

doi:10.1007/s11705-012-1220-6

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 146-151 https://doi.org/10.1007/s11705-012-1220-6

RESEARCH ARTICLE

Biological pretreatment of corn stover by solid state fermentation of Phanerochaete chrysosporium

Jian ZHANG, Xin REN, Wenqun CHEN, Jie BAO(

)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

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Abstract

Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the supermolecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes (LiP and MnP) were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are useful to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.

Keywords biological pretreatment Phanerochaete chrysosporium solid state fermentation biorecalcitrance hydrolysis yield

Corresponding Author(s): BAO Jie,Email:jbao@ecust.edu.cn

Issue Date: 05 June 2012

Cite this article:

Jian ZHANG,Xin REN,Wenqun CHEN, et al. Biological pretreatment of corn stover by solid state fermentation of Phanerochaete chrysosporium[J]. Front Chem Sci Eng, 2012, 6(2): 146-151.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1220-6
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/146

Fig.1 Time courses in the submerged liquid culture of . (a) glucose and dry cell mass (DCM); (b) enzyme activity. Conditions: 30°C, 150 rpm, 100 mL of the nitrogen limited medium described in Material and Methods

Fig.2 Time courses of the hydrolysis yield and water content of corn stover during the solid state fermentation with . (a) without water supplement; (b) 10% of the total water supplemented daily in the whole process. 30°C, initial water content: 60% (w/w)

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