Direct ethanol production from rice straw by coculture with two high-performing fungi

doi:10.1007/s11705-012-1281-6

Front Chem Sci Eng

2012, Vol. 6

Issue (2) : 139-145 https://doi.org/10.1007/s11705-012-1281-6

RESEARCH ARTICLE

Direct ethanol production from rice straw by coculture with two high-performing fungi

Maki TAKANO, Kazuhiro HOSHINO(

)

Graduate School of Science and Engineering, University of Toyama, Toyama 930-8555, Japan

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Abstract

To develop efficient and economical direct ethanol production from fine rice straw crashed mechanically, two high-performing fungi, which can secret hyperactive cellulases and/or ferment effectively various sugars, were selected from some strains belong to Mucor circinelloides preserved in our laboratory. The simultaneous saccharification and fermentation (SSF) by coculture with these fungi was investigated. The screening of high-performing fungi resulted in the selection of NBRC 4572 as an ethanol-producing fungus and NBRC 5398 as a cellulase-secreting fungus. The strain 4572 produced ethanol aerobically from glucose and xylose in high yields of 0.420 g/g at 36 h and 0.478 g/g at 60 h, respectively, but secreted fairly low cellulases. On the other hand, the strain 5398 also produced ethanol from glucose in yield of 0.340 g/g though it had a little growth in xylose culture. However, it secreted hyperactive cellulases that are essential for hydrolysis of rice straw in culture and the maximum activities of endo-β-glucanase and β-glucosidase were 2.11 U/L and 1.47 U/L, respectively. In SSF of rice straw by coculture with two fungi selected, the ethanol production reached 1.28 g/L after 96 h when the inoculation ratio of the strain 5398 to the strain 4572 was 9.

Keywords Mucor circinelloides ethanol production cellulase secretion SSF coculture rice straw

Corresponding Author(s): HOSHINO Kazuhiro,Email:khoshino@eng.u-toyama.ac.jp

Issue Date: 05 June 2012

Cite this article:

Maki TAKANO,Kazuhiro HOSHINO. Direct ethanol production from rice straw by coculture with two high-performing fungi[J]. Front Chem Sci Eng, 2012, 6(2): 139-145.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-012-1281-6
https://academic.hep.com.cn/fcse/EN/Y2012/V6/I2/139

Fig.1 Ethanol production from glucose and xylose by several species of

Fig.2 Cellulases secreted in broth by several species of

Fig.3 Batch culture of NBRC 4572 and 5398 by using glucose (A, C) and xylose (B, D) as carbon sources

Fig.4 Cellulase and hemicellulase secreted in broth by NBRC 4572 and 5398 as batch cultures was performed with glucose and xylose

Fig.5 Direct ethanol production from mechanical crashed rice straw by SSF with a coculture combined with cellulase-secreting fungus (strain 5398) and ethanol-producing fungus (strain 4572)

Fig.6 Enzymatic activities existed in broth as direct ethanol production from fine mechanically crashed rice straw by SSF with coculture

1	Summersa M D, Jenkinsa B M, Hydeb P R, Williamsc J F, Muttersd R G, Scardaccie S C, Haire M W. Biomass production and allocation in rice with implications for straw harvesting and utilization. Biomass and Bioenergy , 2003, 24(3): 163–173 doi: 10.1016/S0961-9534(02)00132-0
2	Ahamed A, Vermette P. Culture-based strategies to enhance cellulase enzyme production from Trichoderma reesei RUT-C30 in bioreactor culture conditions. Biochemical Engineering Journal , 2008, 40(3): 399–407 doi: 10.1016/j.bej.2007.11.030
3	Bhat M K, Bhat S. Cellulose degrading enzymes and their potential industrial applications. Biotechnology Advances , 1997, 15(3–4): 583–620 doi: 10.1016/S0734-9750(97)00006-2
4	Abedinifar S, Karimi K, Khanahmadi M, Taherzadeh M J. Ethanol production by Mucor indicus and Rhizopus oryzae from rice straw by separate hydrolysis and fermentation. Biomass and Bioenergy , 2009, 33(5): 828–833 doi: 10.1016/j.biombioe.2009.01.003
5	Karimi K, Emtiazi G, Taherzadeh M J. Ethanol production from dilute-acid pretreated rice straw by simultaneous saccharification and fermentation with Mucor indicus, Rhizopus oryzae, and Saccharomyces cerevisiae. Enzyme and Microbial Technology , 2006, 40(1): 138–144 doi: 10.1016/j.enzmictec.2005.10.046
6	Arima K, Iwasaki S, Tamura G. Milk clotting enzyme from microorganisms. Part I. Screening test and the identification of the potent fungus. Agricultural and Biological Chemistry , 1967, 31(5): 540–545 doi: 10.1271/bbb1961.31.540
7	Kavadiaa A, Komaitis M, Chevalot I, Blanchard F, Marc I, Aggelis G. Lipid and γ-linolenic acid accumulation in strains of Zygomycetes growing on glucose. Journal of the American Oil Chemists’ Society , 2001, 78(4): 341–346 doi: 10.1007/s11746-001-0266-3
8	Sues A, Millati R, Edebo L, Taherzadeh M J. Ethanol production from hexoses, pentoses, and dilute-acid hydrolyzate by Mucor indicus. FEMS Yeast Research , 2005, 5(6–7): 669–676 doi: 10.1016/j.femsyr.2004.10.013
9	Karimi K, Emtiazi G, Taherzadeh M J. Production of ethanol and mycelial biomass from rice straw hemicellulose hydrolyzate by Mucor indicus. Process Biochemistry (Barking, London, England) , 2006, 41(3): 653–658 doi: 10.1016/j.procbio.2005.08.014
10	Millati R, Edebo L, Taherzadeh M J. Performance of Rhizopus, Rhizomucor, and Mucor in ethanol production from glucose, xylose, and wood hydrolyzates. Enzyme and Microbial Technology , 2005, 36(2–3): 294–300 doi: 10.1016/j.enzmictec.2004.09.007
11	Lübbehüsen T L, Nielsen J, McIntyre M. Aerobic and anaerobic ethanol production by Mucor circinelloides during submerged growth. Applied Microbiology and Biotechnology , 2004, 63: 543–548 doi: 10.1007/s00253-003-1394-4
12	Efremenko E N, Stepanov N A, Nikolskaya A B, Senko O V, Spiricheva O V, Varfolomeev S D. Biocatalysts based on immobilized cells of microorganisms in the production of bioethanol and biobutanol. Catalysis in Industry , 2011, 3(1): 41–46 doi: 10.1134/S207005041101003X
13	Ueng P P, Gong C S. Ethanol production from pentoses and sugar-cane bagasse hemicellulose hydrolysate by Mucor and Fusariumspecies. Enzyme and Microbial Technology , 1982, 4(3): 169–171 doi: 10.1016/0141-0229(82)90111-9
14	Rangel-Porras R A, Meza-Carmen V, Martinez-Cadena G, Torres-Guzman J C, Gonzalez-Hernandez G A, Arnau J, Gutierrez-Corona J F. Molecular analysis of an NAD-dependent alcohol dehydrogenase from the zygomycete Mucor circinelloides. Molecular Genetics and Genomics , 2005, 274(4): 354–363 doi: 10.1007/s00438-005-0025-4
15	Takó M, Tóth A, Nagy L G, Krisch J, Vágv?lgyi C, Papp T. A new β-glucosidase gene from the zygomycete fungus Rhizomucor miehei. Antonie van Leeuwenhoek , 2010, 97(1): 1–10 doi: 10.1007/s10482-009-9382-z
16	Somkuti G A, Babel F J, Somkuti A C. Cellulolysis by Mucor pusillus. Applied Microbiology , 1969, 17(6): 888–892
17	Persson I, Tjerneld F, Hahn-H?gerdal B. Fungal cellulolytic enzyme production. Process Biochemistry (Barking, London, England) , 1991, 26(2): 65–74 doi: 10.1016/0032-9592(91)80019-L
18	Sugimoto M, Suzuki Y. Molecular cloning, sequencing, and expression of a cDNA encoding α-glucosidase from Mucor javanicus. Journal of Biochemistry , 1996, 119: 500–505
19	Takii Y, Ikeda K, Sato C, Yano M, Sato T, Konno H. Production and characterization of β-glucosidase from Rhizopus oryzae MIBA348. Journal of Biological Macromolecules , 2005, 5(1): 11–16
20	Baba Y, Shimonaka A, Koga J, Kubota H, Kono T. Alternative splicing produces two endoglucanases with one or two carbohydrate-binding modules in Mucor circinelloides. Journal of Bacteriology , 2005, 187(9): 3045–3051 doi: 10.1128/JB.187.9.3045-3051.2005
21	Saha B C. Xylanase from a newly isolated Fusarium verticillioides capable of utilizing corn fiber xylan. Applied Microbiology and Biotechnology , 2001, 56(5–6): 762–766 doi: 10.1007/s002530100716
22	Saha B C. Hemicellulose bioconversion. Journal of Industrial Microbiology & Biotechnology , 2003, 30(5): 279–291 doi: 10.1007/s10295-003-0049-x
23	Saha B C. Production, purification and properties of endoglucanase from a newly isolated strain of Mucor circinelloides. Process Biochemistry (Barking, London, England) , 2004, 39(12): 1871–1876 doi: 10.1016/j.procbio.2003.09.013
24	Panagiotou G, Topakas E, Moukouli M, Christakopoulos P, Olsson L. Studying the ability of Fusariumoxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw. Biomass and Bioenergy , 2011, 35(8): 3727–3732 doi: 10.1016/j.biombioe.2011.05.005
25	Saddler J N, Chan M K H, Louis-Seize G. A one step process for the conversion of cellulose to ethanol using anaerobic microorganisms in mono- and co-culture. Biotechnology Letters , 1981, 3(6): 321–326 doi: 10.1007/BF00127402
26	Verma G, Nigam P, Singh D, Chaudhary K. Bioconversion of starch to ethanol in a single-step process by coculture of amylolytic yeasts and Saccharomyces cerevisiae 21. Bioresource Technology , 2000, 72(3): 261–266 doi: 10.1016/S0960-8524(99)00117-0
27	Bernalier A, Fonty G, Bonnemoy F, Gouet P. Degradation and fermentation of cellulose by the rumen anaerobic fungi in axenic cultures or in association with cellulolytic bacteria. Current Microbiology , 1992, 25(3): 143–148 doi: 10.1007/BF01571022
28	Abouzied M M, Reddy C A. Direct fermentation of potato starch to ethanol by cocultures of Aspergillus niger and Saccharomyces cerevisiae. Applied and Environmental Microbiology , 1986, 52(5): 1055–1059
29	Thomas K N G, Ben-Bassat A, Zeikus J G. Ethanol production by thermophilic bacteria: fermentation of cellulosic substrates by cocultures of Clostridium thermocellum and Clostridium thermohydrosulfuricum. Applied and Environmental Microbiology , 1981, 41(6): 1337–1343
30	Alam M Z, Nassereldeen A, Kabbashi A, Nahdatul S, Hussin I S. Production of bioethanol by direct bioconversion of oil-palm industrial effiuent in a stirred-tank bioreactor. Journal of Industrial Microbiology & Biotechnology , 2009, 36(6): 801–808 doi: 10.1007/s10295-009-0554-7
31	Sun R, Lawther J M, Banks W B. Fractional and structural characterization of wheat straw hemicelluloses. Carbohydrate Polymers , 1996, 29(4): 325–331 doi: 10.1016/S0144-8617(96)00018-5
32	J?rgensen H, M?rkeberg A, Krogh K B R, Olsson L. Production of cellulases and hemicellulases by three Penicillium species: effect of substrate and evaluation of cellulase adsorption by capillary electrophoresis. Enzyme and Microbial Technology , 2005, 36(1): 42–48 doi: 10.1016/j.enzmictec.2004.03.023

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