Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(<italic>N</italic>-isopropylacrylamide) in coil and globule states for tissue engineering application

doi:10.1007/s11705-013-1355-0

Front Chem Sci Eng

2013, Vol. 7

Issue (4) : 388-400 https://doi.org/10.1007/s11705-013-1355-0

RESEARCH ARTICLE

Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application

Yang YU¹, Hong ZHANG²(

), Hong SUN³, Dandan XING¹, Fanglian YAO¹(

)

1. School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China; 2. Consolidated Research Institute for Advanced Science and Medical Care, Waseda University (ASMeW), Shinjuku-ku, Tokyo 162-0041, Japan; 3. Basic Medical College, Hebei United University, Tangshan 063000, China

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Abstract

With the excellent biocompatibility and osteoconductivity, nano-hydroxyapatite (nHA) has shown significant prospect in the biomedical applications. Controlling the size, crystallinity and surface properties of nHA crystals is a critical challenge in the design of HA based biomaterials. With the graft copolymer of chitosan and poly(N-isopropylacrylamide) in coil and globule states as a template respectively, a novel composite from chitosan-g-poly(N-isopropylacrylamide) and nano-hydroxyapatite (CS-g-PNIPAM/nHA) was prepared via co-precipitation. Zeta potential analysis, thermogravimetric analysis and X-ray diffraction were used to identify the formation mechanism of the CS-g-PNIPAM/nHA composite and its morphology was observed by transmission electron microscopy. The results suggested that the physical aggregation states of the template polymer could induce or control the size, crystallinity and morphology of HA crystals in the CS-g-PNIPAM/nHA composite. The CS-g-PNIPAM/nHA composite was then introduced to chitosan-gelatin (CS-Gel) polyelectronic complex and the cytocompatibility of the resulting CS-Gel/composite hybrid film was evaluated. This hybrid film was proved to be favorable for the proliferation of MC 3T3-E1 cells. Therefore, the CS-g-PNIPAM/nHA composite is a potential biomaterial in bone tissue engineering.

Keywords chitosan poly(N-isopropylacrylamide) hydroxyapatite coil globule bone tissue engineering

Corresponding Author(s): ZHANG Hong,Email:yaofanglian@tju.edu.cn; YAO Fanglian,Email:hzhang@aoni.waseda.jp

Issue Date: 05 December 2013

Cite this article:

Yang YU,Hong ZHANG,Hong SUN, et al. Nano-hydroxyapatite formation via co-precipitation with chitosan-g-poly(N-isopropylacrylamide) in coil and globule states for tissue engineering application[J]. Front Chem Sci Eng, 2013, 7(4): 388-400.

URL:

https://academic.hep.com.cn/fcse/EN/10.1007/s11705-013-1355-0
https://academic.hep.com.cn/fcse/EN/Y2013/V7/I4/388

Tab.1 CS-g-PNIPAM/nHA composites and their preparation conditions

Fig.1 H NMR spectra of (A) MBSA-CS complex, (B) MBSA-CS-RAFT, (C) MBSA-CS-g-PNIPAM copolymer, and (D) CS-g-PNIPAM in CS-g-PNIPAM/nHA composite in DMSO- at 400 MHz

Fig.2 FT-IR spectra of (A) MBSA-CS complex, (B) MBSA-CS-RAFT, (C) MBSA-CS-g-PNIPAM copolymer, and (D) CS-g-PNIPAM/nHA composite

Fig.3 Proposed mechanism for nHA crystalline formation in situ with CS-g-PNIPAM in coil and globule states as a template

Fig.4 TG curves for (A) composite-1-25 and (B) composite-1-40

Fig.5 Zeta potential for CS-g-PNIPAM/nHA composites and pure nHA

Fig.6 XRD patterns of (A) composite-5-25, (B) composite-10-25, (C) composite-5-40, (D) composite-10-40, and (E) nHA

Fig.7 The effects of reaction temperature and feeding ratio of nHA precursors on the crystal structure of nHA in CS-g-PNIPAM/nHA composites: (a) the average crystallite size, (b) the crystallinity, and (c) the draw ratio

Fig.8 TEM images of (a) composite-1-25, (b) composite-1-40, and (c) nHA

Fig.9 SEM images of cross-sections of (a) CS-Gel/composite-5-25 hybrid film, (b) CS-Gel/composite-5-40 hybrid film, (c) CS-Gel/nHA hybrid film, and (d) CS-Gel film

Fig.10 Proliferation levels of MC 3T3-E1 over a 14-day period. Cells were seeded initially at a density of 2.0 × 10 cells/mL and were observed on CS-Gel/composite films and CS-Gel/nHA films with respect to CS-Gel films (***<0.001)

Fig.11 SEM images of MC 3T3-E1 cultured for 14 days on (a) CS-Gel, (b) CS-Gel/nHA, (c) CS-Gel/composite-5-25, and (d) CS-Gel/composite-5-40 films

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