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A simple umbelliferone based fluorescent probe for the detection of nitroreductase |
Adam C. Sedgwick1, Alex Hayden2, Barry Hill2, Steven D. Bull1, Robert B. P. Elmes2(), Tony D. James1() |
1. Department of Chemistry, University of Bath, Bath, BA2 7AY, UK 2. Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland |
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Abstract A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.
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Corresponding Author(s):
Robert B. P. Elmes,Tony D. James
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Just Accepted Date: 30 November 2017
Online First Date: 15 January 2018
Issue Date: 09 May 2018
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