Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

doi:10.1007/s11783-009-0018-3

Front Envir Sci Eng Chin

2009, Vol. 3

Issue (2) : 194-199 https://doi.org/10.1007/s11783-009-0018-3

RESEARCH ARTICLE

Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Hongyuan WANG^1,2(

), Xiaolu JIANG¹, Ya HE³, Huashi GUAN¹

1. School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; 2. School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; 3. School of Environment, Beijing Normal University, Beijing 100875, China

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Abstract

Four typical coastal sites (rocky shore, sandy shore, mud flat shore, and artificial harbor) at the Yellow Sea were chosen to investigate the spatial and seasonal variations in bacterial communities. This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S rDNA fragments. Two kinds of tetrameric restriction enzymes, HhaI and MspI, were used in the experiment to depict the bacterial community diversity in different marine environments. It was found that the community compositions digested by the two enzymes separately were different. However, the results of bacterial community diversity derived from them were similar. The MDA analysis results of T-RFLP profiles coming from HhaI and MspI both exhibited a significant seasonal community shift for bacteria and a relatively low spatial variation among the four locations. With HhaI as the sample, the pair wise T-tests also revealed that variations were minor between each pair of marine environments, with R ranging from 0.198 to 0.349. However, the bacterial community structure in the mud flat site depicted a larger difference than each of the other three sites (R ranging from 0.282 to 0.349).

Keywords terminal restriction fragment length polymorphism (T-RFLP) bacterial community structure marine microbial diversity HhaI MspI

Corresponding Author(s): WANG Hongyuan,Email:whybnu@163.com

Issue Date: 05 June 2009

Cite this article:

Hongyuan WANG,Xiaolu JIANG,Ya HE, et al. Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis[J]. Front Envir Sci Eng Chin, 2009, 3(2): 194-199.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-009-0018-3
https://academic.hep.com.cn/fese/EN/Y2009/V3/I2/194

Fig.1 (a) site A (b) site D
T-RFLP fingerprints of triplicates from different sites digested with I. (a); (b)

Fig.2 (a) I (b) I
(○) rocky shore, (□) sandy shore, (D) mud flat, (◇) artificial harbor. (a); (b)
MDS plots of T-RFLP patterns from different samples based on Bray-Curtis similarities in 16Sr DNA fragments digested with different enzymes

Tab.1 Observed ANOSIM test values of differences among sites

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