Noncovalent immobilization of MnP from  on carbon nanotubes

doi:10.1007/s11783-009-0041-4

Front.Environ.Sci.Eng.

2009, Vol. 3

Issue (3) : 294-299 https://doi.org/10.1007/s11783-009-0041-4

Research articles

Noncovalent immobilization of MnP from on carbon nanotubes

Jiaxi LI , Xianghua WEN ,

SKLESPC, Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China;

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Abstract Manganese peroxidases (MnP) from Phanerochaete chrysosporium were adsorbed onto multi-walled carbon nanotubes (MWNT). Four different loadings of MnP on MWNTs were investigated, and the maximum enzyme loading of 47.5 µg/mg of MWNTs was obtained in 12 h. The adsorbed MnP showed a catalytic activity of up to 0.1 U/mg of the weight of the system of MnP/MWNTs, with 23% of its original activity retained. The AFM image of the adsorbed enzymes indicated that a layer of MnP covered the surface of the MWNTs and retained its original three-dimensional shape. Amino-based nonspecific interactions may play the dominant role in the adsorption of MnP on MWNTs.

Keywords manganese peroxidases (MnP) Phanerochaete chrysosporium carbon nanotubes immobilization catalytic activity

Issue Date: 05 September 2009

Cite this article:

Jiaxi LI,Xianghua WEN. Noncovalent immobilization of MnP from on carbon nanotubes[J]. Front.Environ.Sci.Eng., 2009, 3(3): 294-299.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-009-0041-4
https://academic.hep.com.cn/fese/EN/Y2009/V3/I3/294

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