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Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor |
Xuan JIA, Hai YAN(), Zijing WANG, Huanju HE, Qianqian XU, Haiou WANG, Chunhua YIN, Liqin LIU |
Department of Biological Science and Technology, University of Science and Technology Beijing, Beijing 100083, China |
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Abstract A promising microalgal strain isolated from fresh water, which can grow both autotrophically on inorganic carbon under lighting and heterotrophically on organic carbon without lighting, was identified as Chlorella sp. USTB-01 with the phylogenetic analysis based on 18S ribosomal ribonucleic acid (rRNA) gene sequences. In the heterotrophic batch culture, more than 20.0 g·L-1 of cell dry weight concentration (DWC) of Chlorella sp. USTB-01 was obtained at day 5, and which was used directly to seed the autotrophic culture. A novel fermentor-helical combined photobioreactor was established and used to cultivate Chlorella sp. USTB-01 for the fixation of carbon dioxide (CO2). It showed that the autotrophic growth of Chlorella sp. USTB-01 in the combined photobioreactor was more effective than that in the fermentor alone and the maximum DWC of 2.5 g·L-1 was obtained at day 6. The highest CO2 fixation of 95% appeared on day 1 in the exponential growth phases of Chlorella sp. USTB-01 and 49.8% protein was found in the harvested microalgal cells.
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Keywords
Chlorella sp. USTB-01
carbon dioxide fixation
combined photobioreactor
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Corresponding Author(s):
YAN Hai,Email:haiyan@ustb.edu.cn
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Issue Date: 05 September 2011
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