|
|
Short-term effect of temperature variation on the competition between PAOs and GAOs during acclimation period of an EBPR system |
Nanqi REN(), Han KANG, Xiuheng WANG, Nan LI |
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China |
|
|
Abstract Sequencing batch reactor (SBR) for enhanced biological phosphorus removal (EBPR) processes was used to investigate the impact of the temperature shock on the competition between phosphorus-accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) in start-up stage. During the 34 days operation, SBR was set with temperature variation(0–5 d, 22±1°C; 6–13 d, 29±1°C; 14–34 d, 14±1°C). PAOs and GAOs were analyzed by fluorescent in situ hybridization (FISH), and intracellular polyphosphate granules were stained by Neisser-stain. The results showed that the influence of temperature shock on PAOs’ abundance was more serious than that on GAOs in the enriching process. Under sudden and substantially temperature variation, from 22±1°C to 29±1°C and then to 14±1°C, the domination of PAOs was deteriorated. After temperature shock, PAOs’ competitive advantages at low temperature that concluded in other study did not appear in our study. As mesophilic, GAOs (indicated by Alphaproteobacteria and Gammaproteobacteria) were more temperature adaptive and better grew and took the domination at 14±1°C in the end. In the competition process, organisms of tetrad forming organisms (TFOs)-like shape which were considered as typical GAOs, were observed. With the evidence of poly-P granules containing by Neisser-straining and result of FISH, these organisms of TFOs-like shape were better to be assumed as adaption state or a special self-protecting shape of PAOs.
|
Keywords
fluorescent in situ hybridization (FISH)
tetrad forming organisms (TFOs)
temperature variation
enhanced biological phosphorus removal (EBPR)
|
Corresponding Author(s):
REN Nanqi,Email:rnq@hit.edu.cn
|
Issue Date: 05 June 2011
|
|
1 |
Panswad T, Doungchai A, Anotai J. Temperature effect on microbial community of enhanced biological phosphorus removal system. Water Research , 2003, 37(2): 409–415 doi: 10.1016/S0043-1354(02)00286-5 pmid:12502069
|
2 |
Lopez-Vazquez C M, Oehmen A, Hooijmans C M, Brdjanovic D, Gijzen H J. Yuan Z, van Loosdrecht M C M. Modeling the PAO-GAO competition: effects of carbon source, pH and temperature. Water Research , 2009, 43(2): 450–462 doi: 10.1016/j.watres.2008.10.032 pmid:19022471
|
3 |
Whang L M, Park J K. Competition between polyphosphate- and glycogen-accumulating organisms in enhanced-biological-phosphorus-removal systems: effect of temperature and sludge age.Water Environment Research , 2006, 78(1): 4–11 doi: 10.2175/106143005X84459 pmid:16553160
|
4 |
Lopez-Vazquez C M, Song Y I, Hooijmans C M, Brdjanovic D, Moussa M S, Gijzen H J, van Loosdrecht M C M. Short-term temperature effects on the anaerobic metabolism of glycogen accumulating organisms. Biotechnology and Bioengineering , 2007, 97(3): 483–495 doi: 10.1002/bit.21302 pmid:17171717
|
5 |
Lopez-Vazquez C M, Song Y I, Hooijmans C M, Brdjanovic D, Moussa M S, Gijzen H J, van Loosdrecht M C M. Temperature effects on the aerobic metabolism of glycogen-accumulating organisms. Biotechnology and Bioengineering , 2008, 101(2): 295–306 doi: 10.1002/bit.21892 pmid:18623226
|
6 |
Erdal U G, Erdal Z K, Randall C W. The competition between PAOs (phosphorus accumulating organisms) and GAOs (glycogen accumulating organisms) in EBPR (enhanced biological phosphorus removal) systems at different temperatures and the effects on system performance. Water Science and Technology , 2003, 47(11): 1–8 pmid:12906264
|
7 |
Lopez-Vazquez C M, Hooijmans C M, Brdjanovic D, Gijzen H J, van Loosdrecht M C M. Temperature effects on glycogen accumulating organisms. Water Research , 2009, 43(11): 2852–2864 doi: 10.1016/j.watres.2009.03.038 pmid:19380157
|
8 |
Li N, Wang X, Ren N, Zhang K, Kang H, You S. Effects of solid retention time (SRT) on sludge characteristics in enhanced biological phosphorus removal (EBPR) reactor. Chemical and Biochemical Engineering Quarterly , 2008, 22: 453–458
|
9 |
APHA. Standard Methods for the Examination of Water and Wastewater. 19th ed. Washington D C: American Public Health Accociation,1995
|
10 |
Amann R I. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Akkermans A D L, Elsas J D, de Bruij F J, eds. Molecular Microbial Ecology Manual vol. 3. 3. 6 . London: Kluwer, 1995: 1–15
|
11 |
Amann R I, Binder B J, Olson R J, Chisholm S W, Devereux R, Stahl D A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Applied and Environmental Microbiology , 1990, 56(6): 1919–1925 pmid:2200342
|
12 |
Daims H, Brühl A, Amann R, Schleifer K H, Wagner M. The domain-specific probe EUB338 is insufficient for the detection of all Bacteria: development and evaluation of a more comprehensive probe set. Systematic and Applied Microbiology , 1999, 22(3): 434–444 pmid:10553296
|
13 |
Crocetti G R, Hugenholtz P, Bond P L, Schuler A, Keller J, Jenkins D, Blackall L L. Identification of polyphosphate-accumulating organisms and design of 16S rRNA-directed probes for their detection and quantitation. Applied and Environmental Microbiology , 2000, 66(3): 1175–1182 doi: 10.1128/AEM.66.3.1175-1182.2000 pmid:10698788
|
14 |
Wong M T, Tan F M, Ng W J, Liu W T. Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic-aerobic activated sludge processes. Microbiology (Reading, England) , 2004, 150(11): 3741–3748 doi: 10.1099/mic.0.27291-0 pmid:15528660
|
15 |
Eikelboom D H, van Buijsen H J J. Microscopic sludge investigation manual, Delft: TNO Research Institute for Environmental Hygiene , 1981
|
16 |
Oehmen A, Lemos P C, Carvalho G, Yuan Z G, Keller J, Blackall L L, Reis M A. Advances in enhanced biological phosphorus removal: from micro to macro scale. Water Research , 2007, 41(11): 2271–2300 doi: 10.1016/j.watres.2007.02.030 pmid:17434562
|
17 |
Levantesi C, Serafim L S, Crocetti G R, Lemos P C, Rossetti S, Blackall L L, Reis M A M, Tandoi V. Analysis of the microbial community structure and function of a laboratory scale enhanced biological phosphorus removal reactor. Environmental Microbiology , 2002, 4(10): 559–569 doi: 10.1046/j.1462-2920.2002.00339.x pmid:12366750
|
18 |
Oehmen A, Zeng R J, Saunders A M, Blackall L L, Keller J, Yuan Z G. Anaerobic and aerobic metabolism of glycogen-accumulating organisms selected with propionate as the sole carbon source. Microbiology (Reading, England) , 2006, 152(9): 2767–2778 doi: 10.1099/mic.0.28065-0 pmid:16946271
|
19 |
Whang L M, Park J K. Competition between polyphosphate- and glycogen-accumulating organisms in biological phosphorus removal systems—effect of temperature. Water Science and Technology , 2002, 46(1-2): 191–194 pmid:12216623
|
20 |
Liu W T, Mino T, Nakamura K, Matsuo T. Glycogen-accumulating population and its anaerobic substrate uptake in anaerobic-aerobic activated sludge without biological phosphorus removal. Water Research , 1996, 30(1): 75–82 doi: 10.1016/0043-1354(95)00121-Z
|
21 |
Cech J S, Hartman P. Competition between polyphosphate and polysaccharide accumulating bacteria in enhanced biological phosphate removal system. Water Research , 1993, 27(7): 1219–1225 doi: 10.1016/0043-1354(93)90014-9
|
22 |
Bond P L, Hugenholtz P, Keller J, Blackall L L. Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors. Applied and Environmental Microbiology , 1995, 61(5): 1910–1916 pmid:7544094
|
23 |
Kang H, Wang X H, Li N, Ren N Q. Characterization of phosphate-accumulating organisms in starting-up EBPR by FISH analysis. Environmental Science , 2009, 30(1): 80–84 pmid:19353861
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|