Effect of pH on biologic degradation of <italic>Microcystis aeruginosa</italic> by alga-lysing bacteria in sequencing batch biofilm reactors

doi:10.1007/s11783-011-0314-6

Front Envir Sci Eng

2012, Vol. 6

Issue (2) : 224-230 https://doi.org/10.1007/s11783-011-0314-6

RESEARCH ARTICLE

Effect of pH on biologic degradation of Microcystis aeruginosa by alga-lysing bacteria in sequencing batch biofilm reactors

Hongjing LI, Mengli HAO, Jingxian LIU, Chen CHEN, Zhengqiu FAN, Xiangrong WANG(

)

Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China

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Abstract

In this paper, the effect of pH on biological degradation of Microcystis aeruginosa by alga-lysing bacteria in laboratory-scale sequencing batch biofilm reactors (SBBRs) was investigated. After 10 d filming with waste activated sludge, the biological film could be formed, and the bioreactors in which laid polyolefin resin filler were used to treat algal culture. By comparing the removal efficiency of chlorophyll a at different aerobic time, the optimum time was determined as 5 h. Under pH 6.5, 7.5, and 8.5 conditions, the removal rates of Microcystis aeruginosa were respectively 75.9%, 83.6%, and 78.3% (in term of chlorophyll a), and that of Chemical Oxygen Demand (COD_Mn) were 30.6%, 35.8%, and 33.5%. While the removal efficiencies of ammonia nitrogen (NH4+-N) were all 100%. It was observed that the sequence of the removal efficiencies of algae, NH4+-N and organic matter were pH 7.5>pH 8.5>pH 6.5. The results showed that the dominant alga-lysing bacteria in the SBBRs was strain HM-01, which was identified as Bacillus sp. by Polymerase Chain Reaction (PCR) amplification of the 16S rRNA gene, Basic Local Alignment Search Tool (BLAST) analysis, and comparison with sequences in the GenBank nucleotide database. The algicidal activated substance which HM-01 strain excreted could withstand high temperature and pressure, also had better hydrophily and stronger polarity.

Keywords pH biological degradation alga-lysing bacteria sequencing batch biofilm reactor (SBBR) 16S rRNA Bacillus sp

Corresponding Author(s): WANG Xiangrong,Email:lihongjing2371@163.com

Issue Date: 01 April 2012

Cite this article:

Hongjing LI,Mengli HAO,Chen CHEN, et al. Effect of pH on biologic degradation of Microcystis aeruginosa by alga-lysing bacteria in sequencing batch biofilm reactors[J]. Front Envir Sci Eng, 2012, 6(2): 224-230.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-011-0314-6
https://academic.hep.com.cn/fese/EN/Y2012/V6/I2/224

Fig.1 Variations of chlorophyll concentration under different pH conditions

Tab.1 Conductivity of different aerobic time in three SBBRs and three blanks ( = 3)

Tab.2 Comparison of chlorophyll , and COD removal efficiency in three SBBRs ( = 3)

Fig.2 Pattern of strain HM-01 under the light microscope

Tab.3 Physiologic and biochemical characteristics of the HM-01 bacterium

Fig.3 Growth curve of the HM-01 bacterium

Fig.4 Amplification of HM-01 16S rDNA. The marker and the molecular weights are 5.0, 3.0, 2.0, 1.0, 0.75, 0.50, 0.25, and 0.10 kb

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