Toxic effects of enrofloxacin on <italic>Scenedesmus obliquus

doi:10.1007/s11783-011-0327-1

Front Envir Sci Eng

2012, Vol. 6

Issue (1) : 107-116 https://doi.org/10.1007/s11783-011-0327-1

RESEARCH ARTICLE

Toxic effects of enrofloxacin on Scenedesmus obliquus

Hongwei QIN, Liufang CHEN, Nan LU, Yahui ZHAO, Xing YUAN(

)

School of Urban and Environmental Science, Northeast Normal University, Changchun 130024, China

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Abstract

In this article, the toxic effects of Enrofloxacin (ENFX) on Scenedesmus obliquus were studied, through investigating the growth, photosynthetic pigments, and protein contents. The possible toxic mechanisms of ENFX were analyzed by determining the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, proline content, and superoxide anion (O2-) generation rate. Results showed that the growth of algae was inhibited by ENFX and the 50% effective concentration (EC₅₀) values for 24, 48, 72, and 96 h of ENFX were 88.39, 63.86, 45.10, and 59.16 mg·L^-1, respectively. After treated with ENFX for 96 h, the contents of photosynthetic pigments decreased with the increase of ENFX concentration, the content of soluble protein and the activity of SOD increased and then decreased, and the generation rate of superoxide anion (O2-) increased continually. The contents of MDA and proline changed little in lower ENFX concentration groups, but increased rapidly when treated with higher concentration groups. These results suggested that ENFX affected the growth of S. obliquus, and the main toxicity mechanism was that algal cells generated the reactive oxygen species under ENFX stress, and then the reactive oxygen species (ROS) induced the oxidation damages of biologic macromolecules and changed the biomembrane permeability further.

Keywords enrofloxacin Scenedesmus obliquus toxic effects

Corresponding Author(s): YUAN Xing,Email:yuanx@nenu.edu.cn

Issue Date: 01 February 2012

Cite this article:

Hongwei QIN,Liufang CHEN,Nan LU, et al. Toxic effects of enrofloxacin on Scenedesmus obliquus[J]. Front Envir Sci Eng, 2012, 6(1): 107-116.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-011-0327-1
https://academic.hep.com.cn/fese/EN/Y2012/V6/I1/107

Fig.1 Relationship between cell density of and optical density

Fig.2 Growth curve of in different concentrations of ENFX

Tab.1 Acute toxicity of ENFX to

Tab.2 Overview of the toxicity data of quinolone antibiotics to algae

Fig.3 Effects of different concentrations of ENFX on photosynthetic pigments contents of (96 h exposure)

Fig.4 Effects of different concentrations of ENFX on soluble protein of (96 h exposure). *, **: compared with the control group, <0.05, <0.01; FW: fresh weight

Fig.5 Effects of ENFX on SOD activity and generation rate of (96 h exposure). FW: fresh weight

Fig.6 Effects of different concentrations of ENFX on MDA contents of (96 h exposure). *, **: compared with the control group, <0.05, <0.01; FW: fresh weight

Fig.7 Effects of different concentrations of ENFX on proline contents of (96 h exposure). *, **: compared with the control group, <0.05, <0.01; FW: fresh weight

Tab.3 Correlations of the physiologic biochemical indexes

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