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Micro-analysis of nitrogen transport and conversion inside activated sludge flocs using microelectrodes |
Lei WANG, Yongtao LV(), Xudong WANG, Yongzhe YANG, Xiaorong BAI |
School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China |
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Abstract To investigate the nitrogen transport and conversion inside activated sludge flocs, micro-profiles of O2, NH4+, NO2–, NO3–, and pH were measured under different operating conditions. The flocs were obtained from a laboratory-scale sequencing batch reactor. Nitrification, as observed from interfacial ammonium and nitrate fluxes, was higher at pH 8.5, than at pH 6.5 and 7.5. At pH 8.5, heterotrophic bacteria used less oxygen than nitrifying bacteria, whereas at lower pH heterotrophic activity dominated. When the ratio of C to N was decreased from 20 to 10, the ammonium uptake increased. When dissolved oxygen (DO) concentration in the bulk liquid was decreased from 4 to 2 mg·L-1, nitrification decreased, and only 25% of the DO influx into the flocs was used for nitrification. This study indicated that nitrifying bacteria became more competitive at a higher DO concentration, a higher pH value (approximately 8.5) and a lower C/N.
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Keywords
nitrogen transport
activated sludge flocs
heterotrophic bacteria
nitrifying bacteria
microelectrodes
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Corresponding Author(s):
LV Yongtao,Email:hybos2000@126.com
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Issue Date: 05 December 2011
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1 |
Okabe S, Satoh H, Watanabe Y. In situ analysis of nitrifying biofilms as determined by in situ hybridization and the use of microelectrodes. Applied and Environmental Microbiology , 1999, 65(7): 3182–3191 pmid:10388720
|
2 |
Satoh H, Ono H, Rulin B, Kamo J, Okabe S, Fukushi K I. Macroscale and microscale analyses of nitrification and denitrification in biofilms attached on membrane aerated biofilm reactors. Water Research , 2004, 38(6): 1633–1641 doi: 10.1016/j.watres.2003.12.020 pmid:15016541
|
3 |
Satoh H, Sasaki Y, Nakamura Y, Okabe S, Suzuki T. Use of microelectrodes to investigate the effects of 2-chlorophenol on microbial activities in biofilms. Biotechnology and Bioengineering , 2005, 91(2): 133–138 doi: 10.1002/bit.20506 pmid:15892057
|
4 |
Chae K J, Rameshwar T, Jang A, Kim S H, Kim I S. Analysis of the nitrifying bacterial community in BioCube sponge media using fluorescent in situ hybridization (FISH) and microelectrodes. Journal of Environmental Management , 2008, 88(4): 1426–1435 doi: 10.1016/j.jenvman.2007.07.016 pmid:17765389
|
5 |
Revsbech N P, Jorgensen B B. Microelectrodes: their use in microbial ecology. Advances in Microbial Ecology , 1986, 9: 293–352
|
6 |
Jensen K, Revsbech N P, Nielsen L P. Microscale distribution of nitrification activity in sediment determined with a shielded microsensor for nitrate. Applied and Environmental Microbiology , 1993, 59(10): 3287–3296 pmid:16349065
|
7 |
de Beer D, Glud A, Epping E, Kühl M. A fast-responding CO2 microelectrode for profiling sediments, microbial mats, and biofilms. Limnology and Oceanography , 1997a, 42(7): 1590–1600 doi: 10.4319/lo.1997.42.7.1590
|
8 |
Ploug H, Jorgensen B B. A net-jet flow system for mass transfer and microsensor studies of sinking aggregates. Marine Ecology Progress Series , 1999, 176: 279–290 doi: 10.3354/meps176279
|
9 |
Satoh H, Nakamura Y, Ono H, Okabe S. Effect of oxygen concentration on nitrification and denitrification in single activated sludge flocs. Biotechnology and Bioengineering , 2003, 83(5): 604–607 doi: 10.1002/bit.10717 pmid:12827702
|
10 |
Li B, Bishop P L. Micro-profiles of activated sludge floc determined using microelectrodes. Water Research , 2004, 38(5): 1248–1258 doi: 10.1016/j.watres.2003.11.019 pmid:14975658
|
11 |
de Beer D, van den Heuvel J C, Ottengraf S P P. Microelectrode measurements of the activity distribution in nitrifying bacterial aggregates. Applied and Environmental Microbiology , 1993, 59(2): 573–579 pmid:16348875
|
12 |
Schramm A, de Beer D, van den Heuvel J C, Ottengraf S, Amann R. Microscale distribution of populations and activities of Nitrosospira and Nitrospira spp. along a macroscale gradient in a nitrifying bioreactor: quantification by in situ hybridization and the use of microsensors. Applied and Environmental Microbiology , 1999a, 65(8): 3690–3696 pmid:10427067
|
13 |
Schramm A, De Beer D, Wagner M, Amann R. Identificationn and activities in situ of Nitrosospira and Nitrospira spp. as dominant population in a nitrifying fluidized bed reactor. Applied and Environmental Microbiology , 1999b, 64(9): 3480–3485
|
14 |
Satoh H, Okabe S, Norimatsu N, Watanabe Y. Significance of substrate C/N ratio on structure and activity of nitrifying biofilms determined by in situ hybridization and the use of microelectrodes. Water Science and Technology , 2000, 41(4-5): 317–321
|
15 |
APHA, AWWA, WEF . Standard Methods for the Examination of Water and Wastewater. 20th ed. Washington DC: American Public Health Association, 1999
|
16 |
Revsbech N P. An oxygen microelectrode with a guard cathode. Limnology and Oceanography , 1989, 34(2): 474–478 doi: 10.4319/lo.1989.34.2.0474
|
17 |
De Beer D, Schramm A, Santegoeds C M, Kühl M. A nitrite microsensor for profiling environmental biofilms. Applied and Environmental Microbiology , 1997b, 63(3): 973–977 pmid:16535560
|
18 |
Grady C P L Jr, Daigger G T, Lim H C. Biological Wastewater Treatment. 2nd ed. Boca Raton: CRC Press 1998
|
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