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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front Envir Sci Eng    2012, Vol. 6 Issue (5) : 701-710    https://doi.org/10.1007/s11783-011-0367-6
RESEARCH ARTICLE
Biodegradation of 2-methylisoborneol by bacteria enriched from biological activated carbon
Rongfang YUAN1, Beihai ZHOU1(), Chunhong SHI1, Liying YU1, Chunlei ZHANG2, Junnong GU2
1. Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; 2. Water Quality Monitoring Center, Beijing Waterworks Group, Beijing 100085, China
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Abstract

One of the most common taste and odour compounds (TOCs) in drinking water is 2-methylisoborneol (2-MIB) which cannot be readily removed by conventional water treatments. Four bacterial strains for degrading 2-MIB were isolated from the surface of a biological activated carbon filter, and were characterized as Micrococcus spp., Flavobacterium spp., Brevibacterium spp. and Pseudomonas spp. based on 16S rRNA analysis. The removal efficiencies of 2-MIB with initial concentrations of 515 ng·L-1 were 98.4%, 96.3%, 95.0%, and 92.8% for Micrococcus spp., Flavobacterium spp., Brevibacterium spp. and Pseudomonas spp., respectively. These removal efficiencies were slightly higher than those with initial concentration at 4.2 mg·L-1 (86.1%, 84.4%, 86.7% and 86.0%, respectively). The kinetic model showed that biodegradation of 2-MIB at an initial dose of 4.2 mg·L-1 was a pseudo-first-order reaction, with rate constants of 0.287, 0.277, 0.281, and 0.294 d-1, respectively. These degraders decomposed 2-MIB to form 2-methylenebornane and 2-methyl-2-bornane as the products.

Keywords 2-methylisoborneol (2-MIB)      biodegradation      2-methylenebornane      2-methyl-2-bornane      pseudo-first-order reaction     
Corresponding Author(s): ZHOU Beihai,Email:zhoubeihai@sina.com   
Issue Date: 01 October 2012
 Cite this article:   
Rongfang YUAN,Beihai ZHOU,Chunhong SHI, et al. Biodegradation of 2-methylisoborneol by bacteria enriched from biological activated carbon[J]. Front Envir Sci Eng, 2012, 6(5): 701-710.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-011-0367-6
https://academic.hep.com.cn/fese/EN/Y2012/V6/I5/701
pHCODMn /(mg·L-1)UV254 /cm-1NH4+-N /(mg·L-1)NO2--N /(mg·L-1)NO3--N /(mg·L-1)
7.11.90.0330.0750.00501.50
Tab.1  The quality of the treated water from a filter
Fig.1  AGE (3%) map of 2-MIB degraders protein expression. M, DL2,000 DNA Marker; lane 1, MIB-1 protein expression; lane 2, MIB-2 protein expression; lane 3, MIB-3 protein expression; lane 4, MIB-4 protein expression.
Fig.2  The neighbor-joining tree showing the phylogenetic relationships of the predominant strains in the isolated culture with the closest-matching species. Accession numbers correspond to partial 16S rRNA gene sequences. Numerical tree values represent bootstrap support. Scale bar represents expected changes per site
Fig.3  Concentrations of 2-MIB in MSM. The initial concentration of 2-MIB in MSM was about 4.2 mg·L
Fig.4  Biomass of 2-MIB degraders. The initial concentration of 2-MIB in MSM was about 4.2mg/L
Fig.5  Concentrations of 2-MIB in the filter influent. The initial concentration of 2-MIB in filter influent was 515 ng·L
Fig.6  Biomass of 2-MIB degraders. The initial concentration of 2-MIB in filter influent was 515 ng·L
Fig.7  Kinetics of 4.2 mg·L 2-MIB biodegradation
Fig.8  Chromatograms of 2-MIB metabolites degraded by bacteria
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