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Effect of illumination on the hydrogen-production capability of anaerobic activated sludge |
Guochen ZHENG1,3(), Jianzheng LI2, Feng ZHAO1, Liguo ZHANG2, Li WEI2, Qiaoying BAN2, Yongsheng ZHAO3 |
1. Songliao River Basin Water Resources Protection Bureau, Changchun 130021, China; 2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; 3. Key Laboratory of Groundwater Resources and Environment (Ministry of Education), Jilin University, Changchun 130021, China |
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Abstract To investigate the influence of illumination on the fermentative hydrogen production system, the hydrogen production efficiencies of two kinds of anaerobic activated sludge (floc and granule) from an anaerobic baffled reactor were detected under visible light, dark and light-dark, respectively. The 10 mL floc sludge or granular sludge was respectively inoculated to 100 mL diluted molasses (chemical oxygen demand of 8000 mg·L-1) in a 250 mL serum bottle, and cultured for 24 h at 37°C under different illumination conditions. The results showed that the floc was more sensitive to illumination than the granule. A hydrogen yield of 19.8 mL was obtained in the dark with a specific hydrogen production rate of 3.52 mol·kg-1MLVSS·d-1 (floc), which was the highest among the three illumination conditions. Under dark condition, the hydrogen yield of floc sludge reached the highest with the specific hydrogen production rate of 3.52 mol·kg-1MLVSS·d-1, and under light-dark, light, the specific hydrogen production rate was 3.11 and 2.21 mol·kg-1MLVSS·d-1, respectively. The results demonstrated that the illumination may affect the dehydrogenase activity of sludge as well as the activity of hydrogen-producing acetogens and then impact hydrogen production capacity.
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Keywords
biohydrogen production
dark fermentation
anaerobic activated sludge
light
dehydrogenase
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Corresponding Author(s):
ZHENG Guochen,Email:wasaizgc@163.com
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Issue Date: 01 February 2012
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1 |
Chen C Y, Chang J S. Enhancing phototropic hydrogen production by solid-carrier assisted fermentation and internal optical-fiber illumination. Process Biochemistry , 2006, 41(9): 2041–2049 doi: 10.1016/j.procbio.2006.05.005
|
2 |
Das D, Veziroglu T N. Advances in biological hydrogen production processes. International Journal of Hydrogen Energy , 2008, 33(21): 6046–6057 doi: 10.1016/j.ijhydene.2008.07.098
|
3 |
Liu B F, Ren N Q, Xing D F, Ding J, Zheng G X, Guo W Q, Xu J F, Xie G J. Hydrogen production by immobilized R. faecalis RLD-53 using soluble metabolites from ethanol fermentation bacteria E. harbinense B49. Bioresource Technology , 2009, 100(10): 2719–2723 doi: 10.1016/j.biortech.2008.12.020 pmid:19200719
|
4 |
Meherkotay S, Das D. Biohydrogen as a renewable energy resource-Prospects and potentials. International Journal of Hydrogen Energy , 2008, 33(1): 258–263 doi: 10.1016/j.ijhydene.2007.07.031
|
5 |
Argun H, Kargi F, Kapdan I K. Light fermentation of dark fermentation effluent for bio-hydrogen production by different Rhodobacter species at different initial volatile fatty acid (VFA) concentrations. International Journal of Hydrogen Energy , 2008, 33(24): 7405–7412 doi: 10.1016/j.ijhydene.2008.09.059
|
6 |
Redwood M D, Macaskie L E. A two-stage, two-organism process for biohydrogen from glucose. International Journal of Hydrogen Energy , 2006, 31(11): 1514–1521 doi: 10.1016/j.ijhydene.2006.06.018
|
7 |
Finster K, Thomsen T R, Ramsing N B. Desulfomusa hansenii gen. nov., sp. nov., a novel marine propionate-degrading, sulfate-reducing bacterium isolated from Zostera marina roots. International Journal of Systematic and Evolutionary Microbiology , 2001, 51(Pt 6): 2055–2061 pmid:11760947
|
8 |
Nath K, Das D. Effect of light intensity and initial pH during hydrogen production by an integrated dark and photofermentation process. International Journal of Hydrogen Energy , 2009, 34(17): 7497–7501 doi: 10.1016/j.ijhydene.2008.11.065
|
9 |
Abildgaard L, Ramsing N B, Finster K. Characterization of the marine propionate-degrading, sulfate-reducing bacterium Desulfofaba fastidiosa sp. nov. and reclassification of Desulfomusa hansenii as Desulfofaba hansenii comb. nov. International Journal of Systematic and Evolutionary Microbiology , 2004, 54(2): 393–399 doi: 10.1099/ijs.0.02820-0 pmid:15023950
|
10 |
Belokopytov B F, Laurinavichius K S, Laurinavichene T V, Ghirardi M L, Seibert M, Tsygankov A A. Towards the integration of dark-and photo-fermentative waste treatment. 2. Optimization of starch-dependent fermentative hydrogen production. International Journal of Hydrogen Energy , 2009, 34(8): 3324–3332 doi: 10.1016/j.ijhydene.2009.02.042
|
11 |
Uyar B, Eroglu I, Yucel M, Gunduz U, Turker L. Effect of light intensity, wavelength and illumination protocol on hydrogen production in photobioreactors. International Journal of Hydrogen Energy , 2007, 32(18): 4670–4677 doi: 10.1016/j.ijhydene.2007.07.002
|
12 |
Guan Y F, Deng M C, Yu X J, Zhang W. Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis. Biochemical Engineering Journal , 2004, 19(1): 69–73 doi: 10.1016/j.bej.2003.10.006
|
13 |
Laurinavichene T, Tekucheva D, Laurinavichius K, Ghirardi M, Seibert M, Tsygankov A. Towards the integration of dark and photo fermentative waste treatment. 1. Hydrogen photoproduction by purple bacterium Rhodobacter capsulatus using potential products of starch fermentation. International Journal of Hydrogen Energy , 2008, 33(23): 7020–7026 doi: 10.1016/j.ijhydene.2008.09.033
|
14 |
Ren N Q, Liu B F, Ding J, Xie G J. Hydrogen production with R. faecalis RLD-53 isolated from freshwater pond sludge. Bioresource Technology , 2009, 100(1): 484–487 doi: 10.1016/j.biortech.2008.05.009 pmid:18573657
|
15 |
Khanal S K, Chen W H, Li L, Sung S W. Biological hydrogen production: effects of pH and intermediate products. International Journal of Hydrogen Energy , 2004, 29: 1123–1131
|
16 |
Sasikala C, Ramana C V, Rao P R. Regulation of simultaneous hydrogen photoproduction during growth by pH and glutamate in Rhodobacter-Sphaeroides Ou-001. International Journal of Hydrogen Energy , 1995, 20(2): 123–126 doi: 10.1016/0360-3199(94)E0009-N
|
17 |
Li H Z, Li B K, Zhu G F, Ren N Q, Bo L X, He J G. Hydrogen production from diluted molasses by anaerobic hydrogen producing bacteria in an anaerobic baffled reactor (ABR). International Journal of Hydrogen Energy , 2007, 32(15): 3274–3283 doi: 10.1016/j.ijhydene.2007.04.023
|
18 |
APHA. Standard Methods for the Examination of Water and Wastewater. 19th ed . Washington DC: American Public Health Association, 1995
|
19 |
Li J Z, Zheng G C, He J G, Chang S, Qin Z. Hydrogen-producing capability of anaerobic activated sludge in three types of fermentations in a continuous stirred-tank reactor. Biotechnology Advances , 2009, 27(5): 573–577 doi: 10.1016/j.biotechadv.2009.04.007 pmid:19393312
|
20 |
Müller N, Schleheck D, Schink B. Involvement of NADH: acceptor oxidoreductase and butyryl coenzyme A dehydrogenase in reversed electron transport during syntrophic butyrate oxidation by Syntrophomonas wolfei. Journal of Bacteriology , 2009, 191(19): 6167–6177 doi: 10.1128/JB.01605-08 pmid:19648244
|
21 |
Yang H W, Jiang Z P, Shi S Q, Jang W Z. INT-dehydrogenase activity test for assessing anaerobic biodegradability of organic compounds. Ecotoxicology and Environmental Safety , 2002, 53(3): 416–421 doi: 10.1016/S0147-6513(02)00002-7 pmid:12485586
|
22 |
Kim M, Baek J, Yun Y, Junsim S, Park S, Kim S. Hydrogen production from Chlamydomonas reinhardtii biomass using a two-step conversion process: anaerobic conversion and photosynthetic fermentation. International Journal of Hydrogen Energy , 2006, 31(6): 812–816 doi: 10.1016/j.ijhydene.2005.06.009
|
23 |
Argun H, Kargi F, Kapdan I K. Hydrogen production by combined dark and light fermentation of ground wheat solution. International Journal of Hydrogen Energy , 2009, 34(10): 4305–4311 doi: 10.1016/j.ijhydene.2009.03.033
|
24 |
Li J Z, Ren N Q, Li B K, Qin Z, He J G. Anaerobic biohydrogen production from monosaccharides by a mixed microbial community culture. Bioresource Technology , 2008, 99(14): 6528–6537 doi: 10.1016/j.biortech.2007.11.072 pmid:18226523
|
25 |
Caravelli A, Giannuzzi L, Zaritzky N. Effect of chlorine on filamentous microorganisms present in activated sludge as evaluated by respirometry and INT-dehydrogenase activity. Water Research , 2004, 38(9): 2395–2404 doi: 10.1016/j.watres.2004.01.044 pmid:15142801
|
27 |
Obbard J P. Measurement of dehydrogenase activity using 2-p-iodophenyl-3-p-nitrophenyl-5-phenyltetrazolium chloride (INT) in the presence of copper. Biology and Fertility of Soils , 2001, 33(4): 328–330 doi: 10.1007/s003740000332
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