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Rapid method for on-site determination of phenolic contaminants in water using a disposable biosensor |
Yuanting LI1, Dawei LI1, Wei SONG1, Meng LI1, Jie ZOU2, Yitao LONG1() |
1. Key Laboratory for Advanced Materials & Department of Chemistry, East China University of Science and Technology, Shanghai 200237, China; 2. Jiangsu Provincial Supervising & Testing Research Institute for Products Quality, Nanjing 210007, China |
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Abstract A disposable biosensor was fabricated using single-walled carbon nanotubes, gold nanoparticles and tyrosinase (SWCNTs-AuNPs-Tyr) modified screen-printed electrodes. The prepared biosensor was applied to the rapid determination of phenolic contaminants within 15 minutes. The SWCNTs-AuNPs-Tyr bionanocomposite sensing layer was characterized with scanning electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry methods. The characterization results revealed that SWCNTs could lead to a high loading of tyrosinase (Tyr) with the large surface area and the porous morphology, while AuNPs could retain the bioactivity of Tyr and enhance the sensitivity. The detection conditions, including working potential, pH of supporting electrolyte and the amount of Tyr were optimumed. As an example, the biosensor for catechol determination displayed a linear range of 8.0 × 10-8 to 2.0 × 10-5 mol·L-1 with a detection limit of 4.5 × 10-8 mol·L-1 (S/N = 3). This method has a rapid response time within 10 s, and shows excellent repeatability and stability. Moreover, the resulting biosensor could be disposable, low-cost, reliable and easy to carry. This kind of new Tyr biosensor provides great potential for rapid, on-site and cost-effective analysis of phenolic contaminants in environmental water samples.
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Keywords
on-site determination
tyrosinase biosensor
phenolic contaminants
single-walled carbon nanotubes
gold nanoparticles
screen-printed electrodes
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Corresponding Author(s):
LONG Yitao,Email:ytlong@ecust.edu.cn
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Issue Date: 01 December 2012
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