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Biosorption of Cu(II) to extracellular polymeric substances (EPS) from Synechoeystis sp.: a fluorescence quenching study |
Xiangliang PAN1(), Jing LIU2, Wenjuan SONG1, Daoyong ZHANG2 |
1. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China; 2. State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China |
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Abstract Biosorption of extracellular polymeric substances (EPS) from Synechocystis sp. (cyanobacterium) with Cu(II) was investigated using fluorescence spectroscopy. Three fluorescence peaks were found in the excitation-emission matrix (EEM) fluorescence spectra of EPS. Fluorescence of peak A (Ex/Em= 275/452 nm) and peak C (Ex/Em= 350/452 nm) were originated from humic-like substances and fluorescence of peak B (Ex/Em= 275/338 nm) was attributed to protein-like substances. Fluorescence of peaks A, B, and C could be quenched by Cu(II). The effective quenching constants (lg Ka) were 2.8–5.84 for peak A, 6.4–9.24 for peak B, and 3.48–6.68 for peak C, respectively. The values of lg Ka showed a decreasing trend with increasing temperature, indicating that the quenching processes were static in nature. The binding constants (lg Kb) followed the order of peak A>peak B>peak C, implying that the humic-like substances in EPS have greater Cu(II) binding capacity than the protein-like substances. The binding site number, n, in EPS-Cu(II) complexes for peaks A, B, and C was less than 1. This suggests the negative cooperativity between multiple binding sites and the presence of more than one Cu binding site.
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Keywords
biosorption
conditional binding constant
extracellular polymeric substances (EPS)
fluorescence quenching
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Corresponding Author(s):
PAN Xiangliang,Email:panxl@ms.xjb.ac.cn
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Issue Date: 01 August 2012
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