Decolorization of azo dyes by a salt-tolerant <italic>Staphylococcus cohnii</italic> strain isolated from textile wastewater

doi:10.1007/s11783-012-0453-4

Front Envir Sci Eng

2012, Vol. 6

Issue (6) : 806-814 https://doi.org/10.1007/s11783-012-0453-4

RESEARCH ARTICLE

Decolorization of azo dyes by a salt-tolerant Staphylococcus cohnii strain isolated from textile wastewater

Bin YAN¹(

), Cuihong DU², Meilan XU¹, Wenchao LIAO¹

1. Department of Environmental Engineering, Xiamen University of Technology, Xiamen 361024, China; 2. School of Biotechnology Engineering, Jimei University, Xiamen 361021, China

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Abstract

The salt-tolerant Staphylococcus cohnii strain, isolated from textile wastewater, has been found effective on decolorizing several kinds of azo dyes with different structures. The optimal conditions for azo dye acid red B (ARB) decolorization by S. cohnii were determined to be pH= 7.0 and 30°C. The decolorization efficiency increased with the increase of the salinity concentration, and around 90% of ARB (100 mg·L^-1) could be decolorized in 24 h when the salinity concentration was up to 50 g·L^-1. Moreover, the strain could still decolorize 19% of ARB in 24 h even when the NaCl concentration was increased to 150 g·L^-1. Meanwhile, the dependence of the specific decolorization rate by S. cohnii on the ARB concentration could be described with Michaelis-Menten kinetics (K_m = 585.7 mg·L^-1, V_max = 109.8 mg·g cell^-1·h^-1). The addition of quinone redox mediator, named 2-hydroxy-1,4-naphthoquinone and anthraquinone-2,6-disulfonate, significantly accelerated the decolorization performance of S. cohnii. Furtherly, the activities of azoreductase (0.55 μmol·mg protein^-1·min^-1) and Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase (8.9 μmol·mg protein^-1·min^-1) have been observed in the crude cell extracts of S. cohnii. The decolorization products of ARB were analyzed by HPLC-MS, and the results indicated the reductive pathway was responsible for azo dye decolorization by S. cohnii.

Keywords Staphylococcus cohnii decolorization salt azoreductase Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase

Corresponding Author(s): YAN Bin,Email:yanb@xmut.edu.cn

Issue Date: 01 December 2012

Cite this article:

Bin YAN,Cuihong DU,Meilan XU, et al. Decolorization of azo dyes by a salt-tolerant Staphylococcus cohnii strain isolated from textile wastewater[J]. Front Envir Sci Eng, 2012, 6(6): 806-814.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0453-4
https://academic.hep.com.cn/fese/EN/Y2012/V6/I6/806

Fig.1 Structures of azo dyes used in this study

Fig.2 Note: The numbers at the nodes indicate the bootstrap values. Bar (0.001), 1 nucleotide substitution per 1000 nucleotides of 16S rDNA sequence. Strain names are shown next to the organism names and GenBank accession numbers for the 16S rDNA sequences used are given in parentheses
he phylogenetic tree of 16S rDNA sequences showing the position of the strain AZR based on neighbor-joining (NJ) with 1000 bootstrap replication.

Fig.3 SEM images (a: 7.6 mm×3.50 k, b for 7.7 mm ×50.0 k) of the isolated strain

Fig.4 Decolorization of different azo dyes by

Fig.5 Effects of (a) pH value (pH 5.0-10.0), (b) temperature (24°C-37°C) and (c) salinity (0-200 g·L) on ARB decolorization by

Fig.6 Relationship between the specific decolorization rate of and ARB concentration

Fig.7 Effects of quinone redox mediators (lawsone and AQDS) on ARB decoloriz-ation by

Tab.1 Effects of ARB solution on the growth of plants before and after decolorization

Fig.8 Pathway for the biodegradation of ARB (Note: MW means molecular weight)

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