Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones and their analogs during biofilm formation

doi:10.1007/s11783-013-0492-5

Front Envir Sci Eng

2014, Vol. 8

Issue (2) : 205-214 https://doi.org/10.1007/s11783-013-0492-5

RESEARCH ARTICLE

Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones and their analogs during biofilm formation

Zhuoying WU, Qing WANG, Feng GUO, Shenghua ZHANG, Qipei JIANG(

), Xin YU(

)

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China

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Abstract

Often as a result of biofilm formation, drinking water distribution systems (DWDS) are regularly faced with the problem of microbial contamination. Quorum sensing (QS) systems play a marked role in the regulation of microbial biofilm formation; thus, inhibition of QS systems may provide a promising approach to biofilm formation control in DWDS. In the present study, 22 bacterial strains were isolated from drinking water-related environments. The following properties of the strains were investigated: bacterial biofilm formation capacity, QS signal molecule N-acyl-L-homoserine lactones (AHLs) production ability, and responses to AHLs and AHL analogs, 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and 2(5H)-furanone. Four AHLs were added to developed biofilms at dosages ranging from 0.1?nmol·L^-1 to 100 nmol·L^-1. As a result, the biofilm growth of more than 1/4 of the isolates, which included AHL producers and non-producers, were significantly promoted. Further, the biofilm biomasses were closely associated with respective AHLs concentrations. These results provided evidence to support the idea that AHLs play a definitive role in biofilm formation in many of the studied bacteria. Meanwhile, two AHLs analogs demonstrated unexpectedly minimal negative effects on biofilm formation. This suggested that, in order to find an applicable QS inhibition approach for biofilm control in DWDS, the testing and analysis of more analogs is needed.

Keywords drinking water distribution systems (DWDS) biofilm quorum sensing (QS) N-acyl-L-homoserine lactones (AHLs) (dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) 2(5H)-furanone

Corresponding Author(s): JIANG Qipei,Email:qpjiang@iue.ac.cn; YU Xin,Email:xyu@iue.ac.cn

Issue Date: 01 April 2014

Cite this article:

Zhuoying WU,Qing WANG,Feng GUO, et al. Responses of bacterial strains isolated from drinking water environments to N-acyl-L-homoserine lactones and their analogs during biofilm formation[J]. Front Envir Sci Eng, 2014, 8(2): 205-214.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-013-0492-5
https://academic.hep.com.cn/fese/EN/Y2014/V8/I2/205

Tab.1 Molecular identi?cation, AHL production and biofilm formation characterization of strains isolated from drinking water environments

Fig.1 Biofilm biomass of strains isolated from drinking water environments

Tab.2 Classification of strains from drinking water environments

Fig.2 Responses of bacterial biofilms to AHL addition: (a) strain 18-Re6; (b) strain 07-Re3; (c) strain 17-Re4; (d) strain 04-T2; (e) strain 06-Re2; (f) strain 05-T3; (g) strain 03-Ri2

Fig.3 Responses of bacterial biofilms to MX addition

Fig.4 Responses of bacterial biofilms to 2(5H)-furanone addition

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