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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

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2018 Impact Factor: 3.883

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Front.Environ.Sci.Eng.    2014, Vol. 8 Issue (5) : 776-783    https://doi.org/10.1007/s11783-013-0586-0
RESEARCH ARTICLE
N2O emission from a sequencing batch reactor for biological N and P removal from wastewater
Lei SHEN1,Yuntao GUAN1,Guangxue WU1,*(),Xinmin ZHAN2
1. Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China
2. Civil Engineering, College of Engineering and Informatics, National University of Ireland, Galway, Ireland
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Abstract

Nitrous oxide (N2O) is a greenhouse gas that can be released during biological nitrogen removal from wastewater. N2O emission from a sequencing batch reactor (SBR) for biological nitrogen and phosphorus removal from wastewater was investigated, and the aims were to examine which process, nitrification or denitrification, would contribute more to N2O emission and to study the effects of heterotrophic activities on N2O emission during nitrification. The results showed that N2O emission was mainly attributed to nitrification rather than to denitrification. N2O emission during denitrification mainly occurred with stored organic carbon as the electron donor. During nitrification, N2O emission was increased with increasing initial ammonium or nitrite concentrations. The ratio of N2O emission to the removed ammonium nitrogen (N2O-N/NH4-N) was 2.5% in the SBR system with high heterotrophic activities, while this ratio was in the range from 0.14% to 1.06% in batch nitrification experiments with limited heterotrophic activities.
Keywords biological nutrient removal      denitrification      greenhouse gas      nitrification      nitrous oxide     
Corresponding Author(s): Guangxue WU   
Issue Date: 20 June 2014
 Cite this article:   
Lei SHEN,Yuntao GUAN,Guangxue WU, et al. N2O emission from a sequencing batch reactor for biological N and P removal from wastewater[J]. Front.Environ.Sci.Eng., 2014, 8(5): 776-783.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-013-0586-0
https://academic.hep.com.cn/fese/EN/Y2014/V8/I5/776
Fig.1  Dynamics of N and P in a typical SBR operation cycle
Fig.2  Dynamics of C, N and P at different C/N ratios. The initial acetate concentrations were 250 mg·L-1 (a), 500 mg·L-1 (b) and 1000 mg·L-1 (c), respectively
C/N ratioduration/minNO3-N reduction rate/(mg·g-1·h-1)NO2-N production rate/ (mg·g-1·h-1)N2O-N emission rate/ (mg·g-1·h-1)N2O-N/NO3-N/%
0.750-20-53.8835.250.010.02%
0.7530-90-2.881.350.3712.79%
1.50-45-63.7944.010.010.01%
1.560-90-12.661.240.030.24%
30-90-67.2347.540.010.01%
Tab.1  Kinetics of N2O emission during denitrification at different C/N ratios.
Fig.3  Dynamics of nitrogen at different NH4-N concentrations. The initial NH4-N concentrations were 7.7 mg·L-1 (a), 17.6 mg·L-1 (b), 36.1 mg·L-1 (c) and 52.4 mg·L-1 (d), respectively
NH4-N/( mg·L-1)NH4-N reduction rate/ (mg·g-1·h-1)NO2-N production rate/(mg·g-1·h-1)NO3-N production rate/(mg·g-1·h-1)N2O-N emission rate/(mg·g-1·h-1)N2O-N/NH4-N/%
7.7-9.893.305.650.010.14%
17.6-9.343.616.050.030.36%
36.1-9.183.855.340.040.49%
52.4-8.754.784.500.090.99%
Tab.2  Kinetics of N2O emission at different initial NH4-N concentrations during nitrification.
Fig.4  Dynamics of nitrogen at different initial NO2-N concentrations. The initial NO2-N concentrations were 0.8 (a), 5.2 (b), 10.4 (c) and 20.5 mg·L-1 (d), respectively
NO2-N/ (mg·L-1)NH4-N reduction rate/(mg·g-1·h-1)NO2-N production rate/(mg·g-1·h-1)NO3-N production rate/(mg·g-1·h-1)N2O-N emission rate/(mg·g-1·h-1)N2O-N/NH4-N/%
0.8-11.325.067.020.040.39%
5.2-11.125.317.040.060.51%
10.4-10.654.646.810.070.69%
20.5-10.365.356.840.111.06%
Tab.3  Kinetics of N2O emission at different initial NO2-N concentrations during nitrification.
Fig.5  Dynamics of NOx-N and N2O under conditions with (With C) or without (No C) heterotrophic activities
1 IPCC. Climate Change 2001: the Scientific Basis, Cambridge: Cambridge University Press, UK, 2001
2 KhalilM A K, RasmussenR A. The global sources of nitrous oxide. Journal of Geophysical Research, 1992, 97(D13): 14651-14660
doi: 10.1029/92JD01222
3 LawY, YeL, PanY, YuanZ. Nitrous oxide emissions from wastewater treatment processes. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 2012, 367(1593): 1265-1277
doi: 10.1098/rstb.2011.0317 pmid: 22451112
4 ButlerM D, WangY Y, CartmellE, StephensonT. Nitrous oxide emissions for early warning of biological nitrification failure in activated sludge. Water Research, 2009, 43(5): 1265-1272
doi: 10.1016/j.watres.2008.12.027 pmid: 19167018
5 ItokawaH, HanakiK, MatsuoT. Nitrous oxide production in high-loading biological nitrogen removal process under low COD/N ratio condition. Water Research, 2001, 35(3): 657-664
doi: 10.1016/S0043-1354(00)00309-2 pmid: 11228962
6 KimS W, MiyaharaM, FushinobuS, WakagiT, ShounH. Nitrous oxide emission from nitrifying activated sludge dependent on denitrification by ammonia-oxidizing bacteria. Bioresource Technology, 2010, 101(11): 3958-3963
doi: 10.1016/j.biortech.2010.01.030 pmid: 20138758
7 WunderlinP, MohnJ, JossA, EmmeneggerL, SiegristH. Mechanisms of N2O production in biological wastewater treatment under nitrifying and denitrifying conditions. Water Research, 2012, 46(4): 1027-1037
doi: 10.1016/j.watres.2011.11.080 pmid: 22227243
8 KampschreurM J, TemminkH, KleerebezemR, JettenM S M, van LoosdrechtM C M. Nitrous oxide emission during wastewater treatment. Water Research, 2009, 43(17): 4093-4103
doi: 10.1016/j.watres.2009.03.001 pmid: 19666183
9 AdouaniN, LendormiT, LimousyL, SireO. Effect of the carbon source on N2O emissions during biological denitrification. Resources, Conservation and Recycling, 2010, 54(5): 299-302
doi: 10.1016/j.resconrec.2009.07.011
10 ColliverB B, StephensonT. Production of nitrogen oxide and dinitrogen oxide by autotrophic nitrifiers. Biotechnology Advances, 2000, 18(3): 219-232
doi: 10.1016/S0734-9750(00)00035-5 pmid: 14538109
11 YuR, KampschreurM J, van LoosdrechtM C M, ChandranK. Mechanisms and specific directionality of autotrophic nitrous oxide and nitric oxide generation during transient anoxia. Environmental Science and Technology, 2010, 44(4): 1313-1319
doi: 10.1021/es902794a pmid: 20104886
12 KimD J, KimY. Effect of ammonium concentration on the emission of N(2)O under oxygen-limited autotrophic wastewater nitrification. Journal of Microbiology and Biotechnology, 2011, 21(9): 988-994
doi: 10.4014/jmb.1101.01033 pmid: 21952377
13 WangY, GengJ, GuoG, WangC, LiuS. N2O production in anaerobic/anoxic denitrifying phosphorus removal process: the effect of carbon sources shock. Chemical Engineering Journal, 2011, 172(2-3): 999-1007
doi: 10.1016/j.cej.2011.07.014
14 LemaireR, MeyerR, TaskeA, CrocettiG R, KellerJ, YuanZ. Identifying causes for N2O accumulation in a lab-scale sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal. Journal of Biotechnology, 2006, 122(1): 62-72
doi: 10.1016/j.jbiotec.2005.08.024 pmid: 16198439
15 SmoldersG J F, van der MeijJ, van LoosdrechtM C M, HeijnenJ J. Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence. Biotechnology and Bioengineering, 1994, 43(6): 461-470
doi: 10.1002/bit.260430605 pmid: 18615742
16 APHA. AWWA, WEF. Standard methods for the examination of water and wastewater (20th ed). Washington DC, USA, 1999.
17 KarrD B, WatersJ K, EmerichD W. Analysis of poly-b-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Applied and Environmental Microbiology, 1983, 46(6): 1339-1344
pmid: 16346443
18 RodgersM, WuG. Production of polyhydroxybutyrate by activated sludge performing enhanced biological phosphorus removal. Bioresource Technology, 2010, 101(3): 1049-1053
doi: 10.1016/j.biortech.2009.08.107 pmid: 19765985
19 HanakiK, HongZ, MatsuoT. Production of nitrous oxide gas during denitrification of wastewater. Water Science and Technology, 1992, 26(5-6): 1027-1036
20 KishidaN, KimJ H, KimochiY, NishimuraO, SasakiH, SudoR. Effect of C/N ratio on nitrous oxide emission from swine wastewater treatment process. Water Science and Technology, 2004, 49(5-6): 359-365
pmid: 15137445
21 LiQ, LiP, ZhuP, WuJ, LiangS. Effects of exogenous organic carbon substrates on nitrous oxide emissions during the denitrification process of sequence batch reactors. Environmental Engineering Science, 2008, 25(8): 1221-1228
doi: 10.1089/ees.2007.0172
22 BeunJ J, DircksK, van LoosdrechtM C M, HeijnenJ J. Poly-b-hydroxybutyrate metabolism in dynamically fed mixed microbial cultures. Water Research, 2002, 36(5): 1167-1180
doi: 10.1016/S0043-1354(01)00317-7 pmid: 11905444
23 AhnJ H, KimS, ParkH, RahmB, PagillaK, ChandranK. N2O emissions from activated sludge processes, 2008-2009: results of a national monitoring survey in the United States. Environmental Science and Technology, 2010, 44(12): 4505-4511
doi: 10.1021/es903845y pmid: 20465250
24 KampschreurM J, van der StarW R L, WieldersH A, MulderJ W, JettenM S M, van LoosdrechtM C M. Dynamics of nitric oxide and nitrous oxide emission during full-scale reject water treatment. Water Research, 2008, 42(3): 812-826
doi: 10.1016/j.watres.2007.08.022 pmid: 17920100
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