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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2015, Vol. 9 Issue (2) : 324-333    https://doi.org/10.1007/s11783-014-0627-3
RESEARCH ARTICLE
Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system
Xi CHEN1(),Linjiang YUAN1,*(),Wenjuan LU1,Yuyou LI1,2,Pei LIU1,Kun NIE1
1. School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China
2. Department of Environmental Science, Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
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Abstract

Aerobic granules were formed in a conventional, continuous flow, completely mixed activated sludge system (CMAS). The reactor was inoculated with seed sludge containing few filaments and fed with synthetic municipal wastewater. The settling time of the sludge and the average dissolved oxygen (DO) of the reactor were 2 h and 4.2 mg·L-1, respectively. The reactor was agitated by a stirrer, with a speed of 250 r·min-1, to ensure good mixing.The granular sludge had good settleability, and the sludge volume index (SVI) was between 50 and 90 mL·g-1. The laser particle analyzer showed the diameter of the granules to be between 0.18 and 1.25 mm. A scanning electron microscope (SEM) investigation revealed the predominance of sphere-like and rod-like bacteria, and only few filaments grew in the granules. The microbial community structure of the granules was also analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Sequencing analysis indicated the dominant species were α, β, and γ-Proteobacteria, Bacteroidetes, and Firmicutes. The data from the study suggested that aerobic granules could form, if provided with sufficient number of filaments and high shear force. It was also observed that a high height-to-diameter ratio of the reactor and short settling time were not essential for the formation of aerobic granular sludge.

Keywords aerobic granular sludge      completely mixed activated sludge system (CMAS)      continuous flow      shear force      filamentous bacteria      polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE)     
Corresponding Author(s): Linjiang YUAN   
Online First Date: 26 December 2013    Issue Date: 13 February 2015
 Cite this article:   
Xi CHEN,Linjiang YUAN,Wenjuan LU, et al. Cultivation of aerobic granular sludge in a conventional, continuous flow, completely mixed activated sludge system[J]. Front. Environ. Sci. Eng., 2015, 9(2): 324-333.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-014-0627-3
https://academic.hep.com.cn/fese/EN/Y2015/V9/I2/324
Fig.1  Schematic diagram of the laboratory-scale CMAS reactor
Fig.2  Activated sludge in the reactors designated as: (a) seed sludge of Reactor I, (b) seed sludge of control reactor, (c) Neisser staining of the seed sludge in the control reactor, (d) and (e) granular sludge in Reactor I, (f) flocculated sludge in the control reactor
food ingredient and chemical compoundsconcentration/(mg·L-1)
Na-acetate79.37
potato starch122
peptone17.41
whole milk powder96.86
yeast52.24
Urea91.74
NH4Cl12.75
K2HPO4·3H2O37.98
KH2PO423.4
MgSO4·7H2O41.02
CaCl25
NaHCO325
FeSO4·7H2O10
KI0.06
H3BO30.3
MnSO4·H2O0.1
CoCl2·6H2O0.49
(NH4)6Mo7O24·4H2O0.11
CuSO4·5H2O0.70
ZnSO4·7H2O0.38
Tab.1  Composition of the synthetic municipal wastewater
Fig.3  (a) Changes of mixed liquor suspended solids (MLSS) and sludge volume index (SVI) values in the given time period in both reactors, (b) Changes of chemical oxygen demand (COD) and NH3-N in the given time period in both reactors
Fig.4  Gram and Neisser staining of the sludge in both reactors. (a) Gram staining of granules in Reactor I, (b) Neisser staining of crushed granules in Reactor I, (c) Gram staining of sludge in the control reactor, (d) Neisser staining of sludge in the control reactor
Fig.5  (a) Distribution of particle size of sludge, measured by a laser particle size analysis system, in both reactors on day 61, (b) Surface view of granules in Reactor I, at high magnification, by SEM
Fig.6  (a) Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profile of the microbial community within the aerobic granules, (b) Evolutionary relationship of clones collected from the DGGE gel and their closest neighbors, shown as a phylogenetic tree
bandsubmission number (from NCBI)closest relatives (accession No.)identity/%putative divisionorigin
1KC206008uncultured α-Proteobacterium(JQ919497.1)100α-Proteobacteriaadapted soil microflora for oil degradation
2KC206009uncultured β-Proteobacterium(AM935413.1)99β-Proteobacteriaaliphatic hydrocarbon-contaminated soil
3KC2060010Chryseobacterium sp. bk_48 (HQ538681.1)99Bacteroidetesactivated sludge
4KC2060011uncultured Pseudomonas sp. (HQ658836.1)99γ-Proteobacteriamunicipal wastewater treatment plant
5KC2060012uncultured Rhodobacteraceae bacterium (AF368183.1)100α-Proteobacteriafull-scale wastewater treatment process
6KC2060013Lactococcus raffinolactis (NR_044359.1)100Firmicutesactivated sludge
7KC2060014uncultured Diaphorobacter sp. (JX301649.1)100β-ProteobacteriaUASB reactor treating domestic wastewater
8KC2060015uncultured Bacteroides sp. (KC110186.1)100Bacteroideteswastewater treatment plant
9KC2060016uncultured bacterium (HQ471747.1)100activated sludge process
10KC2060018uncultured Ethanoligenens sp. (GQ183418.1)100Firmicutessediment from surface-flow wetland
11KC2060017uncultured α- Proteobacterium (JN679095.1)95α-Proteobacteriamembrane bioreactor
12KC2060019Pseudomonas sp. (JQ595540.1)100γ-Proteobacteriawastewater treatment system
13KC2060021uncultured Bacteroidetes bacterium (DQ322192.2)100Bacteroidetesactivated sludge degrading phenol
14KC2060020Pseudomonas sp. (JQ749635.1)99γ-Proteobacteriabiologic system treating vinyl chloride
Tab.2  Species identification of selected DGGE bands
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