Improvement of nitrification efficiency by bioaugmentation in sequencing batch reactors at low temperature

doi:10.1007/s11783-014-0668-7

Front. Environ. Sci. Eng.

2014, Vol. 8

Issue (6) : 937-944 https://doi.org/10.1007/s11783-014-0668-7

RESEARCH ARTICLE

Improvement of nitrification efficiency by bioaugmentation in sequencing batch reactors at low temperature

Di CUI¹,Ang LI^1,^*(

),Tian QIU¹,Rui CAI¹,Changlong PANG¹,Jihua WANG²,Jixian YANG¹,Fang MA^1,^*(

),Nanqi REN¹

1. State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China
2. School of Life Science and Technology, Harbin Normal University, Harbin 150025, China

Download: PDF(631 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

Bioaugmentation is an effective method of treating municipal wastewater with high ammonia concentration in sequencing batch reactors (SBRs) at low temperature (10°C). The cold-adapted ammonia- and nitrite- oxidizing bacteria were enriched and inoculated, respectively, in the bioaugmentation systems. In synthetic wastewater treatment systems, the average NH4+-N removal efficiency in the bioaugmented system (85%) was much higher than that in the unbioaugmented system. The effluent NH4+-N concentration of the bioaugmented system was stably below 8 mg·L^-1 after 20 d operation. In municipal wastewater systems with bioaugmentation, the effluent NH4+-N concentration was below 8 mg·L^-1 after 15 d operation. The average NH4+-N removal efficiency in unbioaugmentation system (about 82%) was lower compared with that in the bioaugmentation system. By inoculating the cold-adapted nitrite-oxidizing bacteria (NOB) into the SBRs after 10 d operation, the nitrite concentration decreased rapidly, reducing the NO2--N accumulation effectively at low temperature. The functional microorganisms were identified by PCR-DGGE, including uncultured Dechloromonas sp., uncultured Nitrospira sp., Clostridium sp. and uncultured Thauera sp. The results suggested that the cold-adapted microbial agent of ammonia-oxidizing bacteria (AOB) and NOB could accelerate the start-up and promote achieving the stable operation of the low-temperature SBRs for nitrification.

Keywords nitrification sequencing batch reactors (SBRs) bioaugmentation low temperature

Corresponding Author(s): Ang LI

Online First Date: 07 March 2014 Issue Date: 17 November 2014

Cite this article:

Di CUI,Ang LI,Tian QIU, et al. Improvement of nitrification efficiency by bioaugmentation in sequencing batch reactors at low temperature[J]. Front. Environ. Sci. Eng., 2014, 8(6): 937-944.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-014-0668-7
https://academic.hep.com.cn/fese/EN/Y2014/V8/I6/937

Fig.1 Schematic of the SBR system in this study

Tab.1 Characteristics of the synthetic and municipal wastewater in this study

Tab.2 Designations and locations of the collected samples

Fig.2 Variations in the COD and removal efficiencies using the SBRs for synthetic (a) and municipal (b) wastewater treatment

Fig.3 Variations in the N H 4 + -N and removal efficiencies for synthetic (a) and municipal (b) wastewater treatment in the SBRs

Fig.4 Variations in the N O 3 - - N / N O 2 - -N concentrations for synthetic (a) and municipal (b) wastewater treatment in the SBRs

Fig.5 DGGE fingerprints showing the microorganism population dynamics in the SBRs

1	Henze M, Grady C P L Jr, Gujer W, Marais G R, Matuso T. Activated sludge model No. 1 (Scientific and Technical Reports No. 1). London: IAWPRC, 1987
2	Krishna C, Mark C M, Loosdrecht V. Substrate flux into storage and growth in relation to activated sludge modeling. Water Research, 1999, 33(14): 3149–3161
3	Kishida N, Saeki G, Tsuneda S, Sudo R. Rapid start-up of a nitrifying reactor using aerobic granular sludge as seed sludge. Water Science and Technology, 2012, 65(3): 581–588 pmid: 22258692
4	Guo J B, Wang J H, Cui D, Wang L, Ma F, Chang C C, Yang J X. Application of bioaugmentation in the rapid start-up and stable operation of biological processes for municipal wastewater treatment at low temperatures. Bioresource Technology, 2010, 101(17): 6622–6629 pmid: 20392635
5	Daverey A, Su S H, Huang Y T, Lin J G. Nitrogen removal from opto-electronic wastewater using the simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) process in sequencing batch reactor. Bioresource Technology, 2012, 113: 225–231 pmid: 22237171
6	Zhu G B, Peng Y Z, Wang S Y, Wu S Y, Ma B. Effect of influent flow rate distribution on the performance of step-feed biological nitrogen removal process. Chemical Engineering Journal, 2007, 131: 319–328
7	Guo J B, Ma F, Qu Y Y, Li A, Wang L. Systematical strategies for wastewater treatment and the generated wastes and greenhouse gases in China. Frontiers of Environmental Science and Engineering in China, 2012, 2: 271–279
8	Venkata Mohan S, Chandrashekara Rao N, Krishna Prasad K, Madhavi B T V, Sharma P N. Treatment of complex chemical wastewater in a sequencing batch reactor (SBR) with an aerobic suspended growth configuration. Process Biochemistry, 2005, 40(5): 1501–1508
9	Chang H N, Moon R K, Park B G, Lim S J, Choi D W, Lee W G, Song S L, Ahn Y H. Simulation of sequential batch reactor (SBR) operation for simultaneous removal of nitrogen and phosphorus. Bioprocess and Biosystems Engineering, 2000, 23(5): 513–521
10	Rittmann B E, McCarty P L. Environmental Biotechnology: Principles and Application. New York: McGraw-Hill International Editions, 2001, 470–525
11	Cui D, Li A, Zhang S, Pang C L, Yang J X, Guo J B, Ma F, Wang J H, Ren N Q. Microbial community analysis of three municipal wastewater treatment plants in winter and spring using culture-dependent and culture-independent methods. World Journal of Microbiology and Biotechnology, 2012, 28(6): 2341–2353 pmid: 22806108
12	Karkman A, Mattila K, Tamminen M, Virta M. Cold temperature decreases bacterial species richness in nitrogen-removing bioreactors treating inorganic mine waters. Biotechnology and Bioengineering, 2011, 108(12): 2876–2883 pmid: 21769859
13	Ducey T F, Vanotti M B, Shriner A D, Szogi A A, Ellison A Q. Characterization of a microbial community capable of nitrification at cold temperature. Bioresource Technology, 2010, 101(2): 491–500 pmid: 19734046
14	Singh K S, Viraraghavan T. Effect of temperature on bio-kinetic coefficients in UASB treatment of municipal wastewater. Water, Air, and Soil Pollution, 2002, 136(1–4): 243–254
15	da Motta M, Pons M N, Roche N. Monitoring filamentous bulking in activated sludge systems fed by synthetic or municipal wastewater. Bioprocess and Biosystems Engineering, 2003, 25(6): 387–393 pmid: 13680344
16	Chen S, Ling J, Blancheton J. Nitrification kinetics of biofilm as affected by water quality factors. Aquacultural Engineering, 2006, 34: 179–197
17	Ma F, Guo J B, Zhao L J, Chang C C, Cui D. Application of bioaugmentation to improve the activated sludge system into the contact oxidation system treating petrochemical wastewater. Bioresource Technology, 2009, 100(2): 597–602 pmid: 18768314
18	Zhao L J, Guo J B, Yang J X, Wang L, Ma F. Bioaugmentation as a tool to accelerate the start-up of anoxic-oxic process in a full-scale municipal wastewater treatment plant at low temperature. International Journal of Environment and Pollution, 2009, 37(2–3): 205–215
19	Martin R W Jr, Robert Baillod C, Mihelcic J R. Low-temperature inhibition of the activated sludge process by an industrial discharge containing the azo dye acid black 1. Water Research, 2005, 39(1): 17–28 pmid: 15607160
20	Head M A, Oleszkiewicz J A. Bioaugmentation for nitrification at cold temperatures. Water Research, 2004, 38(3): 523–530 pmid: 14723920
21	Leu S Y, Stenstrom M K. Bioaugmentation to improve nitrification in activated sludge treatment. Water Environment Research, 2010, 82(6): 524–535 pmid: 17910364
22	LaPara T M, Klatt C G, Chen R Y. Adaptations in bacterial catabolic enzyme activity and community structure in membrane-coupled bioreactors fed simple synthetic wastewater. Journal of Biotechnology, 2006, 121(3): 368–380 pmid: 16125815
23	LaPara T M, Nakatsu C H, Pantea L M, Alleman J E. Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE. Water Research, 2002, 36(3): 638–646 pmid: 11827326
24	Watanabe K, Kodama Y, Harayama S. Design and evaluation of PCR primers to amplify bacterial 16S ribosomal DNA fragments used for community fingerprinting. Journal of Microbiological Methods, 2001, 44(3): 253–262 pmid: 11240048
25	APHA. Standard Methods for the Examination of Water and Wastewater. Washington, D C: American Public Health Assoc, 2005
26	State Environmental Protection Administration of China. Discharge standard of pollutants for municipal wastewater treatment plant (GB 18918–2002). Beijing: China Environmental Science Press, 2002 (in Chinese)
27	Qiu Z F, Zhou Q, Yang D H, Li F T. Start-up and commissioning of A2/O municipal wastewater treatment plant. Water and Wastewater Engineering, 2005, 31(9): 30–33 (in Chinese)
28	Ciudad G, Werner A, Bornhardt C, Munoz C, Antileo C. Differential kinetics of ammonia- and nitrite-oxidizing bacteria: a simple kinetic study based on oxygen affinity and proton release during nitrification. Process Biochemistry, 2006, 41(8): 1764–1772
29	Allen J G, Beutel M W, Call D R, Fischer A M. Effects of oxygenation on ammonia oxidation potential and microbial diversity in sediment from surface-flow wetland mesocosms. Bioresource Technology, 2010, 101(4): 1389–1392 pmid: 19815408
30	Freitag T E, Chang L, Clegg C D, Prosser J I. Influence of inorganic nitrogen management regime on the diversity of nitrite-oxidizing bacteria in agricultural grassland soils. Applied and Environmental Microbiology, 2005, 71(12): 8323–8334 pmid: 16332819
31	Chen C, Ren N Q, Wang A J, Liu L H, Lee D J. Functional consortium for denitrifying sulfide removal process. Applied Microbiology and Biotechnology, 2010, 86(1): 353–358 pmid: 19956940

[1]	Yi Qian, Weichuan Qiao, Yunhao Zhang. *Toxic effect of sodium perfluorononyloxy-benzenesulfonate on Pseudomonas stutzeri* in aerobic denitrification, cell structure and gene expression**[J]. Front. Environ. Sci. Eng., 2021, 15(5): 100-.
[2]	Boyi Cheng, Yi Wang, Yumei Hua, Kate V. Heal. The performance of nitrate-reducing Fe(II) oxidation processes under variable initial Fe/N ratios: The fate of nitrogen and iron species[J]. Front. Environ. Sci. Eng., 2021, 15(4): 73-.
[3]	Sanjena Narayanasamydamodaran, Jian’e Zuo, Haiteng Ren, Nawnit Kumar. Scrap Iron Filings assisted nitrate and phosphate removal in low C/N waters using mixed microbial culture[J]. Front. Environ. Sci. Eng., 2021, 15(4): 66-.
[4]	Yuxin Li, Jiayin Ling, Pengcheng Chen, Jinliang Chen, Ruizhi Dai, Jinsong Liao, Jiejing Yu, Yanbin Xu. Pseudomonas mendocina LYX: A novel aerobic bacterium with advantage of removing nitrate high effectively by assimilation and dissimilation simultaneously[J]. Front. Environ. Sci. Eng., 2021, 15(4): 57-.
[5]	Guoliang Zhang, Liang Zhang, Xiaoyu Han, Shujun Zhang, Yongzhen Peng. Start-up of PN-anammox system under low inoculation quantity and its restoration after low-loading rate shock[J]. Front. Environ. Sci. Eng., 2021, 15(2): 32-.
[6]	Shengjie Qiu, Jinjin Liu, Liang Zhang, Qiong Zhang, Yongzhen Peng. Sludge fermentation liquid addition attained advanced nitrogen removal in low C/N ratio municipal wastewater through short-cut nitrification-denitrification and partial anammox[J]. Front. Environ. Sci. Eng., 2021, 15(2): 26-.
[7]	Rencheng Zhu, Jingnan Hu, Liqiang He, Lei Zu, Xiaofeng Bao, Yitu Lai, Sheng Su. Effects of ambient temperature on regulated gaseous and particulate emissions from gasoline-, E10- and M15-fueled vehicles[J]. Front. Environ. Sci. Eng., 2021, 15(1): 14-.
[8]	Binbin Sheng, Depeng Wang, Xianrong Liu, Guangxing Yang, Wu Zeng, Yiqing Yang, Fangang Meng. Taxonomic and functional variations in the microbial community during the upgrade process of a full-scale landfill leachate treatment plant – from conventional to partial nitrification-denitrification[J]. Front. Environ. Sci. Eng., 2020, 14(6): 93-.
[9]	Wenrui Guo, Yue Wen, Yi Chen, Qi Zhou. Sulfur cycle as an electron mediator between carbon and nitrate in a constructed wetland microcosm[J]. Front. Environ. Sci. Eng., 2020, 14(4): 57-.
[10]	Zhihao Si, Xinshan Song, Xin Cao, Yuhui Wang, Yifei Wang, Yufeng Zhao, Xiaoyan Ge, Awet Arefe Tesfahunegn. Nitrate removal to its fate in wetland mesocosm filled with sponge iron: Impact of influent COD/N ratio[J]. Front. Environ. Sci. Eng., 2020, 14(1): 4-.
[11]	Aoshuang Jing, Tao Liu, Xie Quan, Shuo Chen, Yaobin Zhang. Enhanced nitrification in integrated floating fixed-film activated sludge (IFFAS) system using novel clinoptilolite composite carrier[J]. Front. Environ. Sci. Eng., 2019, 13(5): 69-.
[12]	Zhenfeng Han, Ying Miao, Jing Dong, Zhiqiang Shen, Yuexi Zhou, Shan Liu, Chunping Yang. Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater[J]. Front. Environ. Sci. Eng., 2019, 13(4): 52-.
[13]	Qinqin Liu, Miao Li, Rui Liu, Quan Zhang, Di Wu, Danni Zhu, Xuhui Shen, Chuanping Feng, Fawang Zhang, Xiang Liu. Removal of trimethoprim and sulfamethoxazole in artificial composite soil treatment systems and diversity of microbial communities[J]. Front. Environ. Sci. Eng., 2019, 13(2): 28-.
[14]	Qinqin Liu, Miao Li, Xiang Liu, Quan Zhang, Rui Liu, Zhenglu Wang, Xueting Shi, Jin Quan, Xuhui Shen, Fawang Zhang. Removal of sulfamethoxazole and trimethoprim from reclaimed water and the biodegradation mechanism[J]. Front. Environ. Sci. Eng., 2018, 12(6): 6-.
[15]	Yujiao Sun, Juanjuan Zhao, Lili Chen, Yueqiao Liu, Jiane Zuo. Methanogenic community structure in simultaneous methanogenesis and denitrification granular sludge[J]. Front. Environ. Sci. Eng., 2018, 12(4): 10-.

Viewed

Full text

Abstract

Cited

Shared

Discussed