Mechanisms behind the accelerated extracellular electron transfer in <i>Geobacter sulfurreducens</i> DL-1 by modifying gold electrode with self-assembled monolayers

doi:10.1007/s11783-015-0793-y

Front. Environ. Sci. Eng.

2016, Vol. 10

Issue (3) : 531-538 https://doi.org/10.1007/s11783-015-0793-y

RESEARCH ARTICLE

Mechanisms behind the accelerated extracellular electron transfer in Geobacter sulfurreducens DL-1 by modifying gold electrode with self-assembled monolayers

Feng ZHANG,Shengsong YU,Jie LI,Wenwei LI,Hanqing YU(

)

CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science & Technology of China, Hefei 230026, China

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Abstract

Modification of electrode surface with carboxylic acid terminated alkanethiol self-assembled monolayers (SAMs) has been found to be an effective approach to improve the extracellular electron transfer (EET) of electrochemically active bacteria (EAB) on electrode surface, but the underlying mechanism behind such enhanced EET remains unclear. In this work, the gold electrodes modified by mercapto-acetic acid and mercapto-ethylamine (Au-COOH, Au-NH₂) were used as anodes in microbial electrolysis cells (MECs) inoculated with Geobacter sulfurreducens DL-1, and their electrochemical performance and the bacteria-electrode interactions were investigated. Results showed that the Fe(CN)63-/4- redox reaction occurred on the Au-NH₂ with a higher rate and a lower resistance than that on the Au or the Au-COOH. Both the MECs with the Au-COOH and Au-NH₂ anodes exhibited a higher current density than that with a bare Au anode. The biofilm formed on the Au-COOH was denser than that on bare Au, while the biofilm on the Au-NH₂ had a greater thickness, suggesting a critical role of direct EET in this system. This work suggests that functional groups such as –COOH and-NH₂ could promote electrode performance by accelerating the direct EET of EAB on electrode surface.

Keywords biofilm extracellular electron transfer (EET) Geobacter sulfurreducens DL-1 gold self-assembled monolayers

Corresponding Author(s): Hanqing YU

Online First Date: 02 June 2015 Issue Date: 05 April 2016

Cite this article:

Feng ZHANG,Shengsong YU,Jie LI, et al. Mechanisms behind the accelerated extracellular electron transfer in Geobacter sulfurreducens DL-1 by modifying gold electrode with self-assembled monolayers[J]. Front. Environ. Sci. Eng., 2016, 10(3): 531-538.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-015-0793-y
https://academic.hep.com.cn/fese/EN/Y2016/V10/I3/531

Fig.1 Current densities of the MECs with the different Au-based electrodes. The inset illustrate the initial 2 h of the result

Fig.2 (a) CVs, and (b) Nyquist plots and equivalent circuit of the Au, Au-COOH and Au-NH₂ electrodes in 5 mmol·L^-1 Fe ( CN ) 6 3 - / 4 - in 1/15 mol·L^-1 phosphate buffer saline solution (pH= 7.0)

Fig.3 Background-subtraction CV curves of the different electrodes in the MECs

Fig.4 SEM images of G. sulfurreducens DL-1 biofilms on the surface of Au ((a) and (c)), Au-COOH ((b) and (d)) and Au-NH₂ electrodes ((e) and (f))

Fig.5 CLSM images of G. sulfurreducens DL-1 biofilms on the surface of Au (a), Au-COOH (b) and Au-NH₂ electrodes (c)

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