Please wait a minute...
Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2016, Vol. 10 Issue (6) : 4    https://doi.org/10.1007/s11783-016-0871-9
RESEARCH ARTICLE
Impact of roxarsone on the UASB reactor performance and its degradation
Mengchuan Shui1,Feng Ji2,Rui Tang1,Shoujun Yuan1,Xinmin Zhan3,Wei Wang1,4(),Zhenhu Hu1,4()
1. School of Civil Engineering, Hefei University of Technology, Hefei 230009, China
2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100081, China
3. College of Engineering and Informatics, National University of Ireland, Galway, Ireland
4. Institute of Water Treatment and Wastes Reutilization, Hefei University of Technology, Hefei 230009, China
 Download: PDF(1328 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract

Impact of continuous ROX addition on performance of UASB reactor was investigated

With continuous ROX addition, severe inhibition to methanogenic activity occurred

ROX addition caused the changes in the morphology and bacterial diversity of AGS

A possible biotransformation pathway of ROX in the UASB reactor was proposed

60%–70% of the arsenic was discharged to the effluent, and 30%–40% was precipitated

Roxarsone (3-nitro-4-hydroxyphenylarsonic acid, ROX) has been widely used for decades as an organoarsenic feed additive to control intestinal parasites and improve feed efficiency in animal production. However, most of the ROX is excreted into the manure, causing arsenic contamination in wastewater. The arsenic compounds are toxic to microorganisms, but the influence of continuous ROX loading on upflow anaerobic sludge blanket (UASB) reactor is still unknown. In this study, the impact of ROX and its degradation products on the performance of the UASB reactor and the degradation and speciation of ROX in the reactor were investigated. The UASB reactor (hydraulic retention time: 1.75 d) was operated using synthetic wastewater supplemented with ROX for a period of 260 days. With continuous ROX addition at 25.0 mg?L–1, severe inhibition to methanogenic activity occurred after 87 days operation accompanied with an accumulation of volatile fatty acids (VFAs) and a decline in pH. The decrease of added ROX concentration to 13.2 mg?L–1 did not mediate the inhibition. As(III), As(V), MMA(V), DMA(V), HAPA and an unknown arsenic compound were detected in the reactor, and a possible biotransformation pathway of ROX was proposed. Mass balance analysis of arsenic indicated that 60%–70% of the arsenic was discharged into the effluent, and 30%–40% was precipitated in the reactor. The results from this study suggest that we need to pay attention to the stability in the UASB reactors treating organoarsenic-contaminated manure and wastewater, and the effluent and sludge from the reactor to avoid diffusion of arsenic contamination.

Keywords Anaerobic digestion      Anaerobic granular sludge (AGS)      Arsenic species      Impact      Roxarsone (ROX)      UASB reactor     
PACS:     
Fund: 
Corresponding Author(s): Wei Wang,Zhenhu Hu   
Issue Date: 13 September 2016
 Cite this article:   
Mengchuan Shui,Feng Ji,Rui Tang, et al. Impact of roxarsone on the UASB reactor performance and its degradation[J]. Front. Environ. Sci. Eng., 2016, 10(6): 4.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-016-0871-9
https://academic.hep.com.cn/fese/EN/Y2016/V10/I6/4
component content /%
moisture 85.20
VS 6.50
Ca 13.80
P 7.75
Mg 0.33
Zn 0.10
Ni 0.05
Cr 0.04
Cu 0.01
Tab.1  Characteristics of the AGS.
Fig.1  UASB reactor performance of R0 ((a1)–(a3)) and R1 ((b1)–(b3)) in terms of pH ((a1), (b1)), VFA variation ((a2), (b2)) and biogas production ((a3), (b3))
Fig.2  Images of AGS surface in the reactors R0 (a) and R1(b)
Fig.3  SEM images of the AGS surface in the control of R0 ((a1)–(a4)), and the ROX addition of R1 ((b1)–(b4)). (a1) and (b1) showing the entire morphology (at 50 × magnification), ((a2), (b2)), ((a3), (b3)) and ((a4), (b4)) showing the surface (at 500 × , 2000 × and 10000 × , respectively)
Fig.4  HAPA concentration (based on arsenic) in the reactor and effluent and ROX concentration (based on arsenic) in the influent of R1 from days 200-260 at Stage III
Fig.5  The average concentration of total arsenic, total inorganic arsenic and As(III) -in the influent, reactor and effluent of R1
Fig.6  The possible transformation pathway of ROX in a UASB reactor
1 P Mangalgiri K, Adak A, Blaney L. Organoarsenicals in poultry litter: detection, fate, and toxicity. Environment International, 2015, 75: 68–80
https://doi.org/10.1016/j.envint.2014.10.022 pmid: 25461415
2 Nachman K E, Raber G, Francesconi K A, Navas-Acien A, Love D C. Arsenic species in poultry feather meal. Science of the Total Environment, 2012, 417-418(417–418): 183–188
https://doi.org/10.1016/j.scitotenv.2011.12.022 pmid: 22244353
3 US Food and Drug Administration. FDA response to citizen petition on arsenic-based animal drugs. Animal & Veterinary. 2013. Available online at: (<Date>accessed October 10, 2013</Date>)
4 Yao L, Huang L, He Z, Zhou C, Li G, Deng X. Phosphate enhances uptake of As species in garland chrysanthemum (C. coronarium) applied with chicken manure bearing roxarsone and its metabolites. Environmental Science and Pollution Research International, 2015, 22(6): 4654–4659
https://doi.org/10.1007/s11356-014-3711-0 pmid: 25328095
5 Wang H L, Hu Z H, Tong Z L, Xu Q, Wang W, Yuan S J. Effect of arsanilic acid on anaerobic methanogenic process: Kinetics, inhibition and biotransformation analysis. Biochemical Engineering Journal, 2014, 91(91): 179–185
https://doi.org/10.1016/j.bej.2014.08.011
6 Yang Z, Peng H, Lu X, Liu Q, Huang R, Hu B, Kachanoski G, Zuidhof M J, Le X C. Arsenic metabolites, including N-acetyl-4-hydroxy-m-arsanilic acid, in chicken litter from a roxarsone-feeding study involving 1600 chickens. Environmental Science & Technology, 2016, 50(13): 6737–6743
https://doi.org/10.1021/acs.est.5b05619 pmid: 26876684
7 Bednar A J, Garbarino J R, Ferrer I, Rutherford D W, Wershaw R L, Ranville J F, Wildeman T R. Photodegradation of roxarsone in poultry litter leachates. Science of the Total Environment, 2003, 302(1-3): 237–245
https://doi.org/10.1016/S0048-9697(02)00322-4 pmid: 12526912
8 Guo Q, Liu L, Hu Z, Chen G. Biological phosphorus removal inhibition by roxarsone in batch culture systems. Chemosphere, 2013, 92(1): 138–142
https://doi.org/10.1016/j.chemosphere.2013.02.029 pmid: 23498058
9 Fisher D J, Yonkos L T, Staver K W. Environmental concerns of roxarsone in broiler poultry feed and litter in Maryland, USA. Environmental Science & Technology, 2015, 49(4): 1999–2012
https://doi.org/10.1021/es504520w pmid: 25608233
10 Zhang W, Xu F, Han J, Sun Q, Yang K. Comparative cytotoxicity and accumulation of Roxarsone and its photodegradates in freshwater Protozoan Tetrahymenathermophila. Journal of Hazardous Materials, 2015, 286: 171–178
https://doi.org/10.1016/j.jhazmat.2015.01.001 pmid: 25577319
11 Kim K W, Bang S, Zhu Y, Meharg A A, Bhattacharya P. Arsenic geochemistry, transport mechanism in the soil-plant system, human and animal health issues. Environment International, 2009, 35(3): 453–454
https://doi.org/10.1016/j.envint.2009.01.001 pmid: 19217665
12 Sharma V K, Sohn M. Aquatic arsenic: toxicity, speciation, transformations, and remediation. Environment International, 2009, 35(4): 743–759
https://doi.org/10.1016/j.envint.2009.01.005 pmid: 19232730
13 Adak A, Mangalgiri K P, Lee J, Blaney L. UV irradiation and UV-H₂O₂ advanced oxidation of the roxarsone and nitarsone organoarsenicals. Water Research, 2015, 70(3): 74–85
https://doi.org/10.1016/j.watres.2014.11.025 pmid: 25514660
14 Zhang F F, Wang W, Yuan S J, Hu Z H. Biodegradation and speciation of roxarsone in an anaerobic granular sludge system and its impacts. Journal of Hazardous Materials, 2014, 279(5): 562–568
https://doi.org/10.1016/j.jhazmat.2014.07.047 pmid: 25108830
15 Chong S, Sen T K, Kayaalp A, Ang H M. The performance enhancements of upflow anaerobic sludge blanket (UASB) reactors for domestic sludge treatment—a state-of-the-art review. Water Research, 2012, 46(11): 3434–3470
https://doi.org/10.1016/j.watres.2012.03.066 pmid: 22560620
16 Liu C, Li J, Wang S, Nies L. A syntrophic propionate-oxidizing microflora and its bioaugmentation on anaerobic wastewater treatment for enhancing methane production and COD removal. Frontiers of Environmental Science & Engineering, 2016, 10(4): 1–9
https://doi.org/10.1007/s11783-016-0856-8
17 Chávez P C, Castillo L R, Dendooven L, Escamilla-Silva E M. Poultry slaughter wastewater treatment with an up-flow anaerobic sludge blanket (UASB) reactor. Bioresource Technology, 2005, 96(15): 1730–1736
https://doi.org/10.1016/j.biortech.2004.08.017 pmid: 15936942
18 Liu L, You Q Y, Gibson V, Huang X, Chen S H, Ye Z L, Liu C X. Treatment of swine wastewater in aerobic granular reactors: comparison of different seed granules as factors. Frontiers of Environmental Science & Engineering, 2015, 9(6): 1139–1148
https://doi.org/10.1007/s11783-015-0823-9
19 Cortinas I, Field J A, Kopplin M, Garbarino J R, Gandolfi A J, Sierra-Alvarez R. Anaerobic biotransformation of roxarsone and related N-substituted phenylarsonic acids. Environmental Science & Technology, 2006, 40(9): 2951–2957
https://doi.org/10.1021/es051981o pmid: 16719096
20 Shi L, Wang W, Yuan S J, Hu Z H. Electrochemical stimulation of microbial roxarsone degradation under anaerobic conditions. Environmental Science & Technology, 2014, 48(14): 7951–7958
https://doi.org/10.1021/es501398j pmid: 24937023
21 Stolz J F, Perera E, Kilonzo B, Kail B, Crable B, Fisher E, Ranganathan M, Wormer L, Basu P. Biotransformation of 3-nitro-4-hydroxybenzene arsonic acid (roxarsone) and release of inorganic arsenic by Clostridium species. Environmental Science & Technology, 2007, 41(3): 818–823
https://doi.org/10.1021/es061802i pmid: 17328188
22 Lu D, Ji F, Wang F, Yuan S, Hu Z H, Chen T. Adsorption and photocatalytic decomposition of roxarsone by TiO₂ and its mechanism. Environmental Science and Pollution Research International, 2014, 21(13): 8025–8035
https://doi.org/10.1007/s11356-014-2729-7 pmid: 24659434
23 Sierra-Alvarez R, Cortinas I, Field J A. Methanogenic inhibition by roxarsone (4-hydroxy-3-nitrophenylarsonic acid) and related aromatic arsenic compounds. Journal of Hazardous Materials, 2010, 175(1-3): 352–358
https://doi.org/10.1016/j.jhazmat.2009.10.010 pmid: 19889499
24 Inskeep W P, Mcdermott T R, Fendorf S, Frankenberger W T. Arsenic (V)/(III) cycling in soils and natural waters: chemical and microbiological processes. In: Environmental chemistry of arsenic. W T Frankenberger, Ed, New York, America: Marcel Dekker. 2002. 183–215.
25 Sierra-Alvarez R, Cortinas I, Yenal U, Field J A. Methanogenic inhibition by arsenic compounds. Applied and Environmental Microbiology, 2004, 70(9): 5688–5691
https://doi.org/10.1128/AEM.70.9.5688-5691.2004 pmid: 15345461
26 MacLeod F A, Guiot S R, Costerton J W. Layered structure of bacterial aggregates produced in an upflow anaerobic sludge bed and filter reactor. Applied and Environmental Microbiology, 1990, 56(6): 1598–1607
pmid: 2383005
27 Yang S C, He Y L, Liu Y H, Chou C, Zhang P X, Wang D Q. Effect of wastewater composition on the calcium carbonate precipitation in upflow anaerobic sludge blanket reactors. Frontiers of Environmental Science & Engineering in China, 2010, 4(2): 142–149
https://doi.org/10.1007/s11783-010-0026-3
28 Yin X X, Chen J, Qin J, Sun G X, Rosen B P, Zhu Y G. Biotransformation and volatilization of arsenic by three photosynthetic cyanobacteria. Plant Physiology, 2011, 156(3): 1631–1638
https://doi.org/10.1104/pp.111.178947 pmid: 21562336
29 Qin J, Rosen B P, Zhang Y, Wang G, Franke S, Rensing C. Arsenic detoxification and evolution of trimethylarsine gas by a microbial arsenite S-adenosylmethionine methyltransferase. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(7): 2075–2080
https://doi.org/10.1073/pnas.0506836103 pmid: 16452170
30 Garbarino J R, Rutherford D W, Wershaw R L. Degradation of roxarsone in poultry litter. In: Arsenic in the environment, Proceedings of the US Geological Survey Workshop. Denver, CO.,2001
31 Pinel-Raffaitin P, Le Hecho I, Amouroux D, Potin-Gautier M. Distribution and fate of inorganic and organic arsenic species in landfill leachates and biogases. Environmental Science & Technology, 2007, 41(13): 4536–4541
https://doi.org/10.1021/es0628506 pmid: 17695893
32 Lin P F, Zhang X J, Yang H W, Li Y, Chen C. Applying chemical sedimentation process in drinking water treatment plant to address the emergent arsenic spills in water sources. Frontiers of Environmental Science & Engineering, 2015, 9(1): 50–57
https://doi.org/10.1007/s11783-014-0733-2
33 Wang H J, Gong W X, Liu R P, Liu H J, Qu J H. Treatment of high arsenic content wastewater by a combined physical–chemical process. Colloids and Surfaces. A, Physicochemical and Engineering Aspects, 2011, 379(1–3): 116–120
https://doi.org/10.1016/j.colsurfa.2010.11.047
34 Marvin-Sikkema F D, de Bont J A M. Degradation of nitroaromatic compounds by microorganisms. Applied Microbiology and Biotechnology, 1994, 42(4): 499–507
https://doi.org/10.1007/BF00173912 pmid: 7765729
35 Spain J C. Biodegradation of nitroaromatic compounds. Annual Review of Microbiology, 1995, 49(2): 523–555
https://doi.org/10.1146/annurev.mi.49.100195.002515 pmid: 8561470
36 Karim K, Gupta S K. Biotransformation of nitrophenols in upflow anaerobic sludge blanket reactors. Bioresource Technology, 2001, 80(3): 179–186
https://doi.org/10.1016/S0960-8524(01)00092-X pmid: 11601541
37 Mestrot A, Xie W Y, Xue X M, Zhu Y G. Arsenic volatilization in model anaerobic biogas digesters. Applied Geochemistry, 2013, 33: 294–297
https://doi.org/10.1016/j.apgeochem.2013.02.023
38 Rosen B P, Liu Z. Transport pathways for arsenic and selenium: a minireview. Environment International, 2009, 35(3): 512–515
https://doi.org/10.1016/j.envint.2008.07.023 pmid: 18789529
[1] FSE-16036-OF-SMC_suppl_1 Download
[1] Qinxue Wen, Shuo Yang, Zhiqiang Chen. Mesophilic and thermophilic anaerobic digestion of swine manure with sulfamethoxazole and norfloxacin: Dynamics of microbial communities and evolution of resistance genes[J]. Front. Environ. Sci. Eng., 2021, 15(5): 94-.
[2] Yueqi Jiang, Jia Xing, Shuxiao Wang, Xing Chang, Shuchang Liu, Aijun Shi, Baoxian Liu, Shovan Kumar Sahu. Understand the local and regional contributions on air pollution from the view of human health impacts[J]. Front. Environ. Sci. Eng., 2021, 15(5): 88-.
[3] Fengping Hu, Yongming Guo. Health impacts of air pollution in China[J]. Front. Environ. Sci. Eng., 2021, 15(4): 74-.
[4] Mona Akbar, Muhammad Farooq Saleem Khan, Ling Qian, Hui Wang. Degradation of polyacrylamide (PAM) and methane production by mesophilic and thermophilic anaerobic digestion: Effect of temperature and concentration[J]. Front. Environ. Sci. Eng., 2020, 14(6): 98-.
[5] Sijia Ai, Hongyu Liu, Mengjie Wu, Guangming Zeng, Chunping Yang. Roles of acid-producing bacteria in anaerobic digestion of waste activated sludge[J]. Front. Environ. Sci. Eng., 2018, 12(6): 3-.
[6] Panagiotis G. Kougias, Irini Angelidaki. Biogas and its opportunities—A review[J]. Front. Environ. Sci. Eng., 2018, 12(3): 14-.
[7] Ming Cheng, Huapeng Qin, Kangmao He, Hongliang Xu. Can floor-area-ratio incentive promote low impact development in a highly urbanized area? —A case study in Changzhou City, China[J]. Front. Environ. Sci. Eng., 2018, 12(2): 8-.
[8] Bai-Hang Zhao, Jie Chen, Han-Qing Yu, Zhen-Hu Hu, Zheng-Bo Yue, Jun Li. Optimization of microwave pretreatment of lignocellulosic waste for enhancing methane production: Hyacinth as an example[J]. Front. Environ. Sci. Eng., 2017, 11(6): 17-.
[9] John C. Radcliffe, Declan Page, Bruce Naumann, Peter Dillon. Fifty Years of Water Sensitive Urban Design, Salisbury, South Australia[J]. Front. Environ. Sci. Eng., 2017, 11(4): 7-.
[10] Haifeng Jia, Zheng Wang, Xiaoyue Zhen, Mike Clar, Shaw L. Yu. China’s Sponge City construction: A discussion on technical approaches[J]. Front. Environ. Sci. Eng., 2017, 11(4): 18-.
[11] Shuhan Zhang, Yingying Meng, Jiao Pan, Jiangang Chen. Pollutant reduction effectiveness of low-impact development drainage system in a campus[J]. Front. Environ. Sci. Eng., 2017, 11(4): 14-.
[12] Hannah Kratky, Zhan Li, Yijun Chen, Chengjin Wang, Xiangfei Li, Tong Yu. A critical literature review of bioretention research for stormwater management in cold climate and future research recommendations[J]. Front. Environ. Sci. Eng., 2017, 11(4): 16-.
[13] Robert G. Traver, Ali Ebrahimian. Dynamic design of green stormwater infrastructure[J]. Front. Environ. Sci. Eng., 2017, 11(4): 15-.
[14] Jinsong Tao, Zijian Li, Xinlai Peng, Gaoxiang Ying. Quantitative analysis of impact of green stormwater infrastructures on combined sewer overflow control and urban flooding control[J]. Front. Environ. Sci. Eng., 2017, 11(4): 11-.
[15] Conor Dennehy, Peadar G. Lawlor, Yan Jiang, Gillian E. Gardiner, Sihuang Xie, Long D Nghiem, Xinmin Zhan. Greenhouse gas emissions from different pig manure management techniques: a critical analysis[J]. Front. Environ. Sci. Eng., 2017, 11(3): 11-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed